APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1000-1:10000 |
1:100-1:1000 |
1:100-1:1000 |
1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2 , HepG2
Product Note
Knockdown/Knockout validation was supported by customer review data.
Predict Reactivity
Bovine, Chicken, Japanese Medaka, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.28 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Grp78. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2037126
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
heat shock protein family A (Hsp70) member 5 , BIP , GRP78 , HEL-S-89n , MIF2
Cellular Localization
Endoplasmic reticulum lumen , Melanosome
Background
When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.[supplied by OMIM]
Database
Research Area
DATA IMAGES
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GTX102580 WB Image
Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX102580 IP Image
Immunoprecipitation of Grp78 protein from HepG2 whole cell extracts using 5 μg of Grp78 antibody [N2C1], Internal (GTX102580). Western blot analysis was performed using Grp78 antibody [N2C1], Internal (GTX102580). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX102580 IHC-P Image
Grp78 antibody [N2C1], Internal detects Grp78 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat kidney.Grp78 stained by Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX102580 IHC-P Image
Grp78 antibody [N2C1], Internal detects Grp78 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.Grp78 stained by Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX102580 IHC-P Image
Grp78 antibody [N2C1], Internal detects Grp78 protein at cytosol on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. Grp78 antibody [N2C1], Internal (GTX102580) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX102580 IHC-P Image
Grp78 antibody [N2C1], Internal detects Grp78 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX102580 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX102580 ICC/IF Image
Grp78 antibody [N2C1], Internal detects Grp78 protein by immunofluorescent analysis.Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Grp78 stained by Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:500.Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX102580 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX102580 WB Image
Whole zebrafish extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.
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GTX102580 IHC-P Image
Grp78 antibody [N2C1], Internal detects Grp78 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat liver.Grp78 stained by Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX102580 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX102580 IHC-P Image
Grp78 antibody [N2C1], Internal detects Grp78 protein at cytoplasm in rat prostate by immunohistochemical analysis. Sample: Paraffin-embedded rat prostate. Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX102580 IHC-P Image
Grp78 antibody [N2C1], Internal detects Grp78 protein at cytosol on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Grp78 antibody [N2C1], Internal (GTX102580) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
REVIEW
Grp78 antibody [N2C1], Internal
Cat. No. GTX102580
Rating
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( Average 4 based on 1 users reviews)
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Application
Western Blot(WB)
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( Average 4 based on 1 users reviews)
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Date :
Anonymous submitted on 05-Jul-2016
Application Tested :
WB
Sample Species :
Hu
Sample :
SAS cells
Amount used :
20μg
Blocking :
5% milk in 0.1% TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr