GeneTex
United States (US)

Grp78 antibody [N2C1], Internal

KO/KD
GTX102580_41045_20160728_WB_shRNA_watermark_19011506_181.jpg
GTX102580_41045_IFA_19011506_426.jpg
GTX102580_41045_20150430_IP_19011506_177.jpg
GTX102580_41045_20151105_WB_M_19011506_553.jpg
GTX102580_42144_20150618_WB_19011506_447.jpg
GTX102580_41045_20151105_WB_R_19011506_150.jpg
GTX102580_42144_20151123_IHC-P_R_19011506_313.jpg
GTX102580_42844_20181221_IHC-P_R_19022001_738.jpg
GTX102580_42844_20181221_IHC-P_M_19022001_126.jpg
GTX102580_41045_IHC_M_2_19011506_560.jpg
GTX102580_41045_IHC_R_19011506_900.jpg
GTX102580_42144_20151123_IHC-P_M_19011506_324.jpg

Cat No. GTX102580

Reference 2
1
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
See all Grp78 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

Specificity/Sensitivity

Knockdown/Knockout validation was supported by customer review data.

Predict Reactivity

Zebrafish, Bovine, Chicken, Japanese Medaka, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.19 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Grp78. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Heat Shock Protein Family A (Hsp70) Member 5,Bip,Grp78,Hel-S-89N,Mif2,Hspa5

Cellular Localization

Endoplasmic reticulum lumen , Melanosome

Background

When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX102580_41045_20160728_WB_shRNA_watermark_19011506_181.jpg

GTX102580 WB Image

KO/KD

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX102580_41045_IFA_19011506_426.jpg

GTX102580 ICC/IF Image

Grp78 antibody [N2C1], Internal detects Grp78 protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Grp78 protein stained by Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:500.
Blue: Hoechst 33342 staining.
GTX102580_41045_20150430_IP_19011506_177.jpg

GTX102580 IP Image

Immunoprecipitation of Grp78 protein from HepG2 whole cell extracts using 5 μg of Grp78 antibody [N2C1], Internal (GTX102580).
Western blot analysis was performed using Grp78 antibody [N2C1], Internal (GTX102580).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
GTX102580_41045_20151105_WB_M_19011506_553.jpg

GTX102580 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX102580_42144_20150618_WB_19011506_447.jpg

GTX102580 WB Image

Grp78 antibody detects Grp78 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody (GTX102580) diluted at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX102580_41045_20151105_WB_R_19011506_150.jpg

GTX102580 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX102580_42144_20151123_IHC-P_R_19011506_313.jpg

GTX102580 IHC-P Image

Grp78 antibody [N2C1], Internal detects Grp78 protein at cytoplasm in rat prostate by immunohistochemical analysis.
Sample: Paraffin-embedded rat prostate.
Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX102580_42844_20181221_IHC-P_R_19022001_738.jpg

GTX102580 IHC-P Image

Grp78 antibody [N2C1], Internal detects Grp78 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Grp78 stained by Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX102580_42844_20181221_IHC-P_M_19022001_126.jpg

GTX102580 IHC-P Image

Grp78 antibody [N2C1], Internal detects Grp78 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Grp78 stained by Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX102580_41045_IHC_M_2_19011506_560.jpg

GTX102580 IHC-P Image

Grp78 antibody [N2C1], Internal detects Grp78 protein at cytosol on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Grp78 antibody [N2C1], Internal (GTX102580) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX102580_41045_IHC_R_19011506_900.jpg

GTX102580 IHC-P Image

Grp78 antibody [N2C1], Internal detects Grp78 protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
Grp78 antibody [N2C1], Internal (GTX102580) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX102580_42144_20151123_IHC-P_M_19011506_324.jpg

GTX102580 IHC-P Image

Grp78 antibody [N2C1], Internal detects Grp78 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
REFERENCE

Application Reference

Chen X et al. Redox Biol 2017; 12 : 987-1003 The role of sodium hydrosulfide in attenuating the aging process via PI3K/AKT and CaMKKβ/AMPK pathways.
Application : WB
Reactivity : Rat
Cueno ME et al. Microb Pathog 2015; Periodontal disease level-butyric acid amounts locally administered in the rat gingival mucosa induce ER stress in the systemic blood.
Application : WB
Reactivity : Rat
Submit a Reference
REVIEW

Grp78 antibody [N2C1], Internal Cat No. GTX102580

Rating
( Average 4 based on 1 users reviews)
Application
Western Blot(WB)
( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 05-Jul-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : SAS cells
Amount used : 20μg
Blocking : 5% milk in 0.1% TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169