Grp78 antibody [N2C1], Internal

Name: Grp78 antibody [N2C1], Internal
Brand: GeneTex
SKU: GTX102580
Price: 329.00 USD
Rating: 4 (1 reviews)

Anti-Grp78 antibody [N2C1], Internal used in Western Blot (WB). GTX102580

Anti-Grp78 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102580

Anti-Grp78 antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX102580

Anti-Grp78 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102580

Cat No. GTX102580

Reference 2

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse, Rat
Package	100 μl ($329), 25 μl ($169)

See all Grp78 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

Specificity/Sensitivity

Knockdown/Knockout validation was supported by customer review data.

Predict Reactivity

Zebrafish, Bovine, Chicken, Japanese Medaka, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.28 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Grp78. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 5 , BIP , GRP78 , HEL-S-89n , MIF2

Cellular Localization

Endoplasmic reticulum lumen , Melanosome

Background

When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.[supplied by OMIM]

Database

Gene ID: 3309 HSPA5
UniProt: P11021 HSPA5

Research Area

DATA IMAGES

GTX102580 WB Image

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102580 ICC/IF Image

Grp78 antibody [N2C1], Internal detects Grp78 protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Grp78 protein stained by Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX102580 IP Image

Immunoprecipitation of Grp78 protein from HepG2 whole cell extracts using 5 μg of Grp78 antibody [N2C1], Internal (GTX102580).
Western blot analysis was performed using Grp78 antibody [N2C1], Internal (GTX102580).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX102580 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102580 WB Image

GTX102580 IHC-P Image

Grp78 antibody [N2C1], Internal detects Grp78 protein at cytoplasm in rat prostate by immunohistochemical analysis.
Sample: Paraffin-embedded rat prostate.
Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102580 IHC-P Image

Grp78 antibody [N2C1], Internal detects Grp78 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Grp78 stained by Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102580 IHC-P Image

Grp78 antibody [N2C1], Internal detects Grp78 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Grp78 stained by Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102580 WB Image

GTX102580 IHC-P Image

Grp78 antibody [N2C1], Internal detects Grp78 protein at cytosol on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Grp78 antibody [N2C1], Internal (GTX102580) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102580 IHC-P Image

Grp78 antibody [N2C1], Internal detects Grp78 protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
Grp78 antibody [N2C1], Internal (GTX102580) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102580 IHC-P Image

Grp78 antibody [N2C1], Internal detects Grp78 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Chen X et al. Redox Biol 2017; 12 : 987-1003 The role of sodium hydrosulfide in attenuating the aging process via PI3K/AKT and CaMKKβ/AMPK pathways.

Application : WB

Reactivity : Rat

Cueno ME et al. Microb Pathog 2015; Periodontal disease level-butyric acid amounts locally administered in the rat gingival mucosa induce ER stress in the systemic blood.

Application : WB

Reactivity : Rat

Submit a Reference

REVIEW