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Grp78 antibody [N2C1], Internal

Anti-Grp78 antibody [N2C1], Internal used in Western Blot (WB). GTX102580
Anti-Grp78 antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX102580
Anti-Grp78 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102580
Anti-Grp78 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102580
Anti-Grp78 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102580
Anti-Grp78 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102580
Anti-Grp78 antibody [N2C1], Internal used in Western Blot (WB). GTX102580
Anti-Grp78 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102580
Anti-Grp78 antibody [N2C1], Internal used in Western Blot (WB). GTX102580
Anti-Grp78 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102580
Anti-Grp78 antibody [N2C1], Internal used in Western Blot (WB). GTX102580
Anti-Grp78 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102580
Anti-Grp78 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102580
Anti-Grp78 antibody [N2C1], Internal used in Western Blot (WB). GTX102580

Cat. No. GTX102580

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat, Zebrafish, Mosquito
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2 , HepG2

Product Note

Knockdown/Knockout validation was supported by customer review data.

Predict Reactivity

Bovine, Chicken, Japanese Medaka, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.28 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Grp78. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037126

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 5 , BIP , GRP78 , HEL-S-89n , MIF2

Cellular Localization

Endoplasmic reticulum lumen , Melanosome

Background

When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Grp78 antibody [N2C1], Internal used in Western Blot (WB). GTX102580

GTX102580 WB Image

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Grp78 antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX102580

GTX102580 IP Image

Immunoprecipitation of Grp78 protein from HepG2 whole cell extracts using 5 μg of Grp78 antibody [N2C1], Internal (GTX102580).
Western blot analysis was performed using Grp78 antibody [N2C1], Internal (GTX102580).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Grp78 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102580

GTX102580 IHC-P Image

Grp78 antibody [N2C1], Internal detects Grp78 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
Grp78 stained by Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Grp78 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102580

GTX102580 IHC-P Image

Grp78 antibody [N2C1], Internal detects Grp78 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Grp78 stained by Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Grp78 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102580

GTX102580 IHC-P Image

Grp78 antibody [N2C1], Internal detects Grp78 protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
Grp78 antibody [N2C1], Internal (GTX102580) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Grp78 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102580

GTX102580 IHC-P Image

Grp78 antibody [N2C1], Internal detects Grp78 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Grp78 antibody [N2C1], Internal used in Western Blot (WB). GTX102580

GTX102580 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Grp78 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102580

GTX102580 ICC/IF Image

Grp78 antibody [N2C1], Internal detects Grp78 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Grp78 stained by Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Grp78 antibody [N2C1], Internal used in Western Blot (WB). GTX102580

GTX102580 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Grp78 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102580

GTX102580 IHC-P Image

Grp78 antibody [N2C1], Internal detects Grp78 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Grp78 stained by Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Grp78 antibody [N2C1], Internal used in Western Blot (WB). GTX102580

GTX102580 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Grp78 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102580

GTX102580 IHC-P Image

Grp78 antibody [N2C1], Internal detects Grp78 protein at cytoplasm in rat prostate by immunohistochemical analysis.
Sample: Paraffin-embedded rat prostate.
Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Grp78 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102580

GTX102580 IHC-P Image

Grp78 antibody [N2C1], Internal detects Grp78 protein at cytosol on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Grp78 antibody [N2C1], Internal (GTX102580) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Grp78 antibody [N2C1], Internal used in Western Blot (WB). GTX102580

GTX102580 WB Image

Whole zebrafish extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

REFERENCE

REVIEW

Grp78 antibody [N2C1], Internal Cat. No. GTX102580

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 05-Jul-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : SAS cells
Amount used : 20μg
Blocking : 5% milk in 0.1% TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169