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Grp78 antibody [N2C1], Internal

Anti-Grp78 antibody [N2C1], Internal used in Western Blot (WB). GTX102580
Anti-Grp78 antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX102580
Anti-Grp78 antibody [N2C1], Internal used in Western Blot (WB). GTX102580
Anti-Grp78 antibody [N2C1], Internal used in Western Blot (WB). GTX102580
Anti-Grp78 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102580
Anti-Grp78 antibody [N2C1], Internal used in Western Blot (WB). GTX102580
Anti-Grp78 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102580
Anti-Grp78 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102580
Anti-Grp78 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102580
Anti-Grp78 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102580
Anti-Grp78 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102580
Anti-Grp78 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102580
Anti-Grp78 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102580

Cat No. GTX102580

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat, Mosquito
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

Product Note

Knockdown/Knockout validation was supported by customer review data.

Predict Reactivity

Zebrafish, Bovine, Chicken, Japanese Medaka, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.28 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Grp78. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037126

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 5 , BIP , GRP78 , HEL-S-89n , MIF2

Cellular Localization

Endoplasmic reticulum lumen , Melanosome

Background

When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Grp78 antibody [N2C1], Internal used in Western Blot (WB). GTX102580

GTX102580 WB Image

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Grp78 antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX102580

GTX102580 IP Image

Immunoprecipitation of Grp78 protein from HepG2 whole cell extracts using 5 μg of Grp78 antibody [N2C1], Internal (GTX102580).
Western blot analysis was performed using Grp78 antibody [N2C1], Internal (GTX102580).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Grp78 antibody [N2C1], Internal used in Western Blot (WB). GTX102580

GTX102580 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Grp78 antibody [N2C1], Internal used in Western Blot (WB). GTX102580

GTX102580 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Grp78 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102580

GTX102580 IHC-P Image

Grp78 antibody [N2C1], Internal detects Grp78 protein at cytoplasm in rat prostate by immunohistochemical analysis.
Sample: Paraffin-embedded rat prostate.
Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Grp78 antibody [N2C1], Internal used in Western Blot (WB). GTX102580

GTX102580 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Grp78 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102580

GTX102580 ICC/IF Image

Grp78 antibody [N2C1], Internal detects Grp78 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Grp78 stained by Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Grp78 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102580

GTX102580 IHC-P Image

Grp78 antibody [N2C1], Internal detects Grp78 protein at cytosol on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Grp78 antibody [N2C1], Internal (GTX102580) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Grp78 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102580

GTX102580 IHC-P Image

Grp78 antibody [N2C1], Internal detects Grp78 protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
Grp78 antibody [N2C1], Internal (GTX102580) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Grp78 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102580

GTX102580 IHC-P Image

Grp78 antibody [N2C1], Internal detects Grp78 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Grp78 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102580

GTX102580 IHC-P Image

Grp78 antibody [N2C1], Internal detects Grp78 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Grp78 stained by Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Grp78 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102580

GTX102580 IHC-P Image

Grp78 antibody [N2C1], Internal detects Grp78 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
Grp78 stained by Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Grp78 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102580

GTX102580 IHC-P Image

Grp78 antibody [N2C1], Internal detects Grp78 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Grp78 stained by Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

Grp78 antibody [N2C1], Internal Cat No. GTX102580

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 05-Jul-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : SAS cells
Amount used : 20μg
Blocking : 5% milk in 0.1% TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf