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Grp78 antibody

Anti-Grp78 antibody used in Western Blot (WB). GTX113340
Anti-Grp78 antibody used in IHC (Paraffin sections) (IHC-P). GTX113340
Anti-Grp78 antibody used in IHC (Paraffin sections) (IHC-P). GTX113340
Anti-Grp78 antibody used in Western Blot (WB). GTX113340
Anti-Grp78 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113340
Anti-Grp78 antibody used in IHC (Paraffin sections) (IHC-P). GTX113340
Anti-Grp78 antibody used in IHC (Paraffin sections) (IHC-P). GTX113340
Anti-Grp78 antibody used in IHC (Paraffin sections) (IHC-P). GTX113340
Anti-Grp78 antibody used in Western Blot (WB). GTX113340
Anti-Grp78 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113340
Anti-Grp78 antibody used in IHC (Paraffin sections) (IHC-P). GTX113340
Anti-Grp78 antibody used in Western Blot (WB). GTX113340
Anti-Grp78 antibody used in Western Blot (WB). GTX113340

Cat No. GTX113340

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat, Chicken
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , NIH-3T3 , JC , BCL-1 , PC-12 , Rat2

Predict Reactivity

Zebrafish, Bovine, Japanese Medaka, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Grp78. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11173017

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 5 , BIP , GRP78 , HEL-S-89n , MIF2

Cellular Localization

Endoplasmic reticulum lumen , Melanosome

Background

When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Grp78 antibody used in Western Blot (WB). GTX113340

GTX113340 WB Image

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody (GTX113340) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Grp78 antibody used in IHC (Paraffin sections) (IHC-P). GTX113340

GTX113340 IHC-P Image

Grp78 antibody detects Grp78 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Grp78 antibody (GTX113340) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Grp78 antibody used in IHC (Paraffin sections) (IHC-P). GTX113340

GTX113340 IHC-P Image

Grp78 antibody detects Grp78 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Grp78 antibody (GTX113340) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Grp78 antibody used in Western Blot (WB). GTX113340

GTX113340 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
GTX113340 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Grp78 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113340

GTX113340 ICC/IF Image

Grp78 antibody detects Grp78 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Grp78 protein stained by Grp78 antibody (GTX113340) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Grp78 antibody used in IHC (Paraffin sections) (IHC-P). GTX113340

GTX113340 IHC-P Image

Grp78 antibody detects Grp78 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
Grp78 stained by Grp78 antibody (GTX113340) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Grp78 antibody used in IHC (Paraffin sections) (IHC-P). GTX113340

GTX113340 IHC-P Image

Grp78 antibody detects Grp78 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Grp78 stained by Grp78 antibody (GTX113340) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Grp78 antibody used in IHC (Paraffin sections) (IHC-P). GTX113340

GTX113340 IHC-P Image

Grp78 antibody detects Grp78 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Grp78 stained by Grp78 antibody (GTX113340) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Grp78 antibody used in Western Blot (WB). GTX113340

GTX113340 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody (GTX113340) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Grp78 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113340

GTX113340 ICC/IF Image

Grp78 antibody detects Grp78 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Grp78 stained by Grp78 antibody (GTX113340) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Grp78 antibody used in IHC (Paraffin sections) (IHC-P). GTX113340

GTX113340 IHC-P Image

Grp78 antibody detects Grp78 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Grp78 stained by Grp78 antibody (GTX113340) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Grp78 antibody used in Western Blot (WB). GTX113340

GTX113340 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody (GTX113340) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Grp78 antibody used in Western Blot (WB). GTX113340

GTX113340 WB Image

Grp78 antibody detects Grp78 protein by western blot analysis.
A. 30 μg PC-12 whole cell extract
B. 30 μg Rat2 whole cell extract
7.5% SDS-PAGE
Grp78 antibody (GTX113340) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

Grp78 antibody Cat No. GTX113340

Rating ( Average 5 based on 5 users reviews)
Application
Western Blot(WB) ( Average 5 based on 5 users reviews)
Date : Anonymous submitted on 05-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Figure Note : 0-30 μM cisplatin treatment for 24hr.
Date : Anonymous submitted on 14-May-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 3 μL
Blocking : 3% BSA in TBST, 4°C, 1Hr
Primary Antibody : 1:10000, 4°C, 12Hr
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2017.PMID: 28234964
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal J Biomed Sci in 2013.PMID: 24359494
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Biomed Res Int in 2020.PMID: 32071920
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf