APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:5000-1:20000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
NIH-3T3 , JC , BCL-1 , PC-12 , Rat2 , HepG2
Predict Reactivity
Zebrafish, Bovine, Japanese Medaka, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS pH7, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.56 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Grp78. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11173017
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
heat shock protein family A (Hsp70) member 5 , BIP , GRP78 , HEL-S-89n , MIF2
Cellular Localization
Endoplasmic reticulum lumen , Melanosome
Background
When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.[supplied by OMIM]
Database
Research Area
DATA IMAGES
|
GTX113340 WB Image
Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody (GTX113340) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX113340 IHC-P Image
Grp78 antibody detects Grp78 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Grp78 antibody (GTX113340) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX113340 IHC-P Image
Grp78 antibody detects Grp78 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Grp78 antibody (GTX113340) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX113340 WB Image
Sample (30 μg of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
GTX113340 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX113340 ICC/IF Image
Grp78 antibody detects Grp78 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Grp78 protein stained by Grp78 antibody (GTX113340) diluted at 1:500.
Blue: Hoechst 33342 staining.
|
|
|
GTX113340 IHC-P Image
Grp78 antibody detects Grp78 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.Grp78 stained by Grp78 antibody (GTX113340) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX113340 IHC-P Image
Grp78 antibody detects Grp78 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat duodenum.Grp78 stained by Grp78 antibody (GTX113340) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX113340 IHC-P Image
Grp78 antibody detects Grp78 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.Grp78 stained by Grp78 antibody (GTX113340) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX113340 IHC-P Image
Grp78 antibody detects Grp78 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat liver.Grp78 stained by Grp78 antibody (GTX113340) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX113340 WB Image
Grp78 antibody detects Grp78 protein by western blot analysis.
A. 30 μg PC-12 whole cell extract
B. 30 μg Rat2 whole cell extract
7.5% SDS-PAGE
Grp78 antibody (GTX113340) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX113340 ICC/IF Image
Grp78 antibody detects Grp78 protein by immunofluorescent analysis.Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Grp78 stained by Grp78 antibody (GTX113340) diluted at 1:500.Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
|
|
|
GTX113340 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody (GTX113340) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
|
|
|
GTX113340 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody (GTX113340) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
REFERENCE
REVIEW
Grp78 antibody
Cat. No. GTX113340
|
Rating
|
|
( Average 5 based on 6 users reviews)
|
Application
|
Western Blot(WB)
|
|
( Average 5 based on 6 users reviews)
|
Date :
Anonymous submitted on 15-Nov-2022
Application Tested :
WB
Sample Species :
Hu
Sample :
CAL-27 shLu treated with Drug
Amount used :
30ug
Blocking :
5%milk/TBST, RT°C, 1Hr
Primary Antibody :
1:1000, 4°C, 18Hr
Date :
Anonymous submitted on 05-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Sample :
U2OS cells
Amount used :
30 μg
Blocking :
5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 12Hr
Figure Note :
0-30 μM cisplatin treatment for 24hr.
Date :
Anonymous submitted on 14-May-2021
Application Tested :
WB
Sample Species :
Hu
Sample :
A549 cells
Amount used :
3 μL
Blocking :
3% BSA in TBST, 4°C, 1Hr
Primary Antibody :
1:10000, 4°C, 12Hr
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Rat
Figure Note :
The data was peer reviewed and published in the journal PLoS One in 2017.
PMID: 28234964
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Rat
Figure Note :
The data was peer reviewed and published in the journal J Biomed Sci in 2013.
PMID: 24359494
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Biomed Res Int in 2020.
PMID: 32071920
