GeneTex
United States (US)

Grp78 antibody

KO/KD
GTX113340_40520_20160728_WB_shRNA_watermark_18121410_332.jpg
GTX113340_42606_20161219_IHC-P_R_18121410_746.jpg
GTX113340_42606_20161219_IHC-P_M_18121410_911.jpg
GTX113340_42964_20170927_IFA_18121410_659.jpg
GTX113340_40520_WB_M_18121410_947.jpg
GTX113340_42963_20170927_IFA_18121410_273.jpg
GTX113340_40520_WB_R_18121410_978.jpg
GTX113340_42963_20170908_WB_18121410_729.jpg
GTX113340_42963_20181221_IHC-P_R_19022001_164.jpg
GTX113340_42963_20181221_IHC-P_M_19022001_143.jpg
GTX113340_42964_20181221_IHC-P_R_19022001_547.jpg
GTX113340_42964_20181221_IHC-P_M_19022001_594.jpg

Cat No. GTX113340

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat, Chicken
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , NIH-3T3 , JC , BCL-1 , PC-12 , Rat2

Predict Reactivity

Zebrafish, Bovine, Japanese Medaka, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.92mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Grp78. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

BIP antibody, FLJ26106 antibody, GRP78 antibody, MIF2 antibody, HSPA5 antibody, immunoglobulin heavy chain-binding protein antibody, 78 kDa glucose-regulated protein antibody, endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody, "heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) antibody"

Cellular Localization

Endoplasmic reticulum lumen , Melanosome

Background

When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX113340_40520_20160728_WB_shRNA_watermark_18121410_332.jpg

GTX113340 WB Image

KO/KD

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody (GTX113340) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113340_42606_20161219_IHC-P_R_18121410_746.jpg

GTX113340 IHC-P Image

Grp78 antibody detects Grp78 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Grp78 antibody (GTX113340) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX113340_42606_20161219_IHC-P_M_18121410_911.jpg

GTX113340 IHC-P Image

Grp78 antibody detects Grp78 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Grp78 antibody (GTX113340) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX113340_42964_20170927_IFA_18121410_659.jpg

GTX113340 ICC/IF Image

Grp78 antibody detects Grp78 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Grp78 protein stained by Grp78 antibody (GTX113340) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX113340_40520_WB_M_18121410_947.jpg

GTX113340 WB Image

Sample (30 µg of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
GTX113340 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113340_42963_20170927_IFA_18121410_273.jpg

GTX113340 ICC/IF Image

Grp78 antibody detects Grp78 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Grp78 protein stained by Grp78 antibody (GTX113340) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX113340_40520_WB_R_18121410_978.jpg

GTX113340 WB Image

Grp78 antibody detects Grp78 protein by western blot analysis.
A. 30 µg PC-12 whole cell extract
B. 30 µg Rat2 whole cell extract
7.5% SDS-PAGE
Grp78 antibody (GTX113340) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113340_42963_20170908_WB_18121410_729.jpg

GTX113340 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody (GTX113340) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113340_42963_20181221_IHC-P_R_19022001_164.jpg

GTX113340 IHC-P Image

Grp78 antibody detects Grp78 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
Grp78 stained by Grp78 antibody (GTX113340) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX113340_42963_20181221_IHC-P_M_19022001_143.jpg

GTX113340 IHC-P Image

Grp78 antibody detects Grp78 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Grp78 stained by Grp78 antibody (GTX113340) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX113340_42964_20181221_IHC-P_R_19022001_547.jpg

GTX113340 IHC-P Image

Grp78 antibody detects Grp78 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Grp78 stained by Grp78 antibody (GTX113340) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX113340_42964_20181221_IHC-P_M_19022001_594.jpg

GTX113340 IHC-P Image

Grp78 antibody detects Grp78 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Grp78 stained by Grp78 antibody (GTX113340) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169