APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:5000-1:20000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Rat heart , Rat Lung , Rat Liver , Rat kidney , NIH-3T-3 , JC , BCL-1 , C2C12 , Raw264.7 , 293T , A431 , HeLa , HepG2
Product Note
Knockdown/Knockout validation was supported by customer review data.
Predict Reactivity
Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.14 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse Grp78. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2885657
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
heat shock protein 5 , AL022860 , AU019543 , Bip , D2Wsu141e , D2Wsu17e , Grp78 , Hsce70 , SEZ-7 , Sez7 , baffled , mBiP
Cellular Localization
Endoplasmic reticulum lumen , Melanosome
Background
Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
Database
Research Area
DATA IMAGES
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GTX127934 ICC/IF Image
Hspa5 antibody detects Hspa5 protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Hspa5 protein stained by Hspa5 antibody (GTX127934) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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GTX127934 IHC-P Image
Hspa5 antibody detects Hspa5 protein at cytosol on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
Hspa5 antibody (GTX127934) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX127934 IHC-P Image
Hspa5 antibody detects Hspa5 protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Hspa5 antibody (GTX127934) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX127934 WB Image
Sample (30 ug of whole cell lysate)
A: NIH-3T-3
B: JC
C: BCL-1
D: C2C12
E: Raw264.7
7.5% SDS PAGE
GTX127934 diluted at 1:10000
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GTX127934 IHC-P Image
Hspa5 antibody detects Hspa5 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Hspa5 antibody (GTX127934) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX127934 WB Image
Hspa5 antibody detects Hspa5 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hspa5 antibody (GTX127934) diluted at a dilution of 1:10000.
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GTX127934 WB Image
Hspa5 antibody detects Hspa5 protein by Western blot analysis.
A. 50 μg Rat heart lysate/extract
B. 50 μg Rat Lung lysate/extract
C. 50 μg Rat Liver lysate/extract
D. 50 μg Rat kidney lysate/extract
7.5 % SDS-PAGE
Hspa5 antibody (GTX127934) dilution: 1:10000
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GTX127934 WB Image
The data was published in the 2018 in Sci Rep. PMID: 29891991
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GTX127934 WB Image
The data was published in the 2019 in J Clin Biochem Nutr. PMID: 30705510
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GTX127934 WB Image
The data was published in the 2019 in J Clin Biochem Nutr. PMID: 30705510
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REFERENCE
REVIEW
Grp78 antibody
Cat. No. GTX127934
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Rating
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( Average 4 based on 1 users reviews)
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Application
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Western Blot(WB)
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( Average 4 based on 1 users reviews)
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Date :
Anonymous submitted on 05-Jul-2016
Application Tested :
WB
Sample Species :
Hu
Sample :
SAS cells
Amount used :
20μg
Blocking :
5% milk in 0.1% TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
