GeneTex
  • Country / Location Selection

United States (US)

Grp78 antibody

Anti-Grp78 antibody used in IHC (Paraffin sections) (IHC-P). GTX127934
Anti-Grp78 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127934
Anti-Grp78 antibody used in IHC (Paraffin sections) (IHC-P). GTX127934
Anti-Grp78 antibody used in Western Blot (WB). GTX127934
Anti-Grp78 antibody used in Western Blot (WB). GTX127934
Anti-Grp78 antibody used in Western Blot (WB). GTX127934
Anti-Grp78 antibody used in IHC (Paraffin sections) (IHC-P). GTX127934

Cat No. GTX127934

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , NIH-3T-3 , JC , BCL-1 , C2C12 , Raw264.7 , Rat heart , Rat Lung , Rat Liver , Rat kidney

Specificity/Sensitivity

Knockdown/Knockout validation was supported by customer review data.

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.139 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse Grp78. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

heat shock protein 5 , AL022860 , AU019543 , Bip , D2Wsu141e , D2Wsu17e , Grp78 , Hsce70 , SEZ-7 , Sez7 , baffled , mBiP

Cellular Localization

Endoplasmic reticulum lumen , Melanosome

Background

Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.

Database

Research Area

DATA IMAGES

Anti-Grp78 antibody used in IHC (Paraffin sections) (IHC-P). GTX127934

GTX127934 IHC-P Image

Hspa5 antibody detects Hspa5 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Hspa5 antibody (GTX127934) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Grp78 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127934

GTX127934 ICC/IF Image

Hspa5 antibody detects Hspa5 protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Hspa5 protein stained by Hspa5 antibody (GTX127934) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Grp78 antibody used in IHC (Paraffin sections) (IHC-P). GTX127934

GTX127934 IHC-P Image

Hspa5 antibody detects Hspa5 protein at cytosol on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
Hspa5 antibody (GTX127934) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Grp78 antibody used in Western Blot (WB). GTX127934

GTX127934 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T-3
B: JC
C: BCL-1
D: C2C12
E: Raw264.7
7.5% SDS PAGE
GTX127934 diluted at 1:10000

Anti-Grp78 antibody used in Western Blot (WB). GTX127934

GTX127934 WB Image

Hspa5 antibody detects Hspa5 protein by Western blot analysis.
A. 50 μg Rat heart lysate/extract
B. 50 μg Rat Lung lysate/extract
C. 50 μg Rat Liver lysate/extract
D. 50 μg Rat kidney lysate/extract
7.5 % SDS-PAGE
Hspa5 antibody (GTX127934) dilution: 1:10000

Anti-Grp78 antibody used in Western Blot (WB). GTX127934

GTX127934 WB Image

Hspa5 antibody detects Hspa5 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hspa5 antibody (GTX127934) diluted at a dilution of 1:10000.

Anti-Grp78 antibody used in IHC (Paraffin sections) (IHC-P). GTX127934

GTX127934 IHC-P Image

Hspa5 antibody detects Hspa5 protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Hspa5 antibody (GTX127934) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

Grp78 antibody Cat No. GTX127934

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 05-Jul-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : SAS cells
Amount used : 20μg
Blocking : 5% milk in 0.1% TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf