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Grp78 antibody

Anti-Grp78 antibody used in Western Blot (WB). GTX22902
Anti-Grp78 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22902
Anti-Grp78 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22902
Anti-Grp78 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22902

Cat. No. GTX22902

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse, Rat, Dog, Hamster, Mosquito
Package
100 μg ($399)
See all Grp78 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2 μg/ml
ICC/IF Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Synthetic peptide corresponding to residues C T(643) G E E D T S E K D E L(654) of rat GRP78.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein 5 , AL022860 , AU019543 , Bip , D2Wsu141e , D2Wsu17e , Grp78 , Hsce70 , SEZ-7 , Sez7 , baffled , mBiP

Cellular Localization

Cytoplasm

Background

The 78 kDa glucose regulated protein/BiP (GRP78) belongs to the family of ~70 kDa heat shock proteins (HSP70). GRP78 is a resident protein of the endoplasmic reticulum (ER) and associates transiently with a variety of newly synthesized secretory and membrane proteins. GRP78 may also interact with mutant and misfolded proteins, marking them for degredation. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C-terminus of GRP78 and other resident ER proteins including glucose regulated protein 94 (GRP94) and protein disulfide isomerase (PDI). The KDEL signal is recognized by the KDEL receptor and this interaction ensures recycling of escaped ER proteins back to the ER.

Database

Research Area

DATA IMAGES

Anti-Grp78 antibody used in Western Blot (WB). GTX22902

GTX22902 WB Image

WB analysis of rat liver lysate using GTX22902 Grp78 antibody.

Anti-Grp78 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22902

GTX22902 ICC/IF Image

ICC/IF analysis of MDCK cells using GTX22902 Grp78 antibody.
Green : Primary antibody
Blue : cell nucleus
Permeabilization : 0.1% Triton X-100
Dilution : 10 μg/ml in blocking buffer for at least 1 hour at room temperature

Anti-Grp78 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22902

GTX22902 ICC/IF Image

ICC/IF analysis of HMVEC Cells using GTX22902 Grp78 antibody.
Red : Primary antibody
Blue : Nuclei

Anti-Grp78 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22902

GTX22902 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX22902 Grp78 antibody.
Red : Primary antibody
Blue : Nuclei

REFERENCE

Application Reference

Caraballo GI et al. J Virol 2022; 96 (12) : e0070422 The Dengue Virus Nonstructural Protein 1 (NS1) Interacts with the Putative Epigenetic Regulator DIDO1 to Promote Flavivirus Replication in Mosquito Cells.
Application : ICC/IF
Reactivity : Mosquito
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399