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Grp78 antibody

Anti-Grp78 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22902
Anti-Grp78 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22902
Anti-Grp78 antibody used in Western Blot (WB). GTX22902
Anti-Grp78 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22902

Cat No. GTX22902

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP

Reactivity

Human, Mouse, Rat, Dog, Hamster
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 2 μg/ml
ICC/IF Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Synthetic peptide corresponding to residues C T(643) G E E D T S E K D E L(654) of rat GRP78.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

heat shock protein 5 , AL022860 , AU019543 , Bip , D2Wsu141e , D2Wsu17e , Grp78 , Hsce70 , SEZ-7 , Sez7 , baffled , mBiP

Cellular Localization

Cytoplasm

Background

The 78 kDa glucose regulated protein/BiP (GRP78) belongs to the family of ~70 kDa heat shock proteins (HSP70). GRP78 is a resident protein of the endoplasmic reticulum (ER) and associates transiently with a variety of newly synthesized secretory and membrane proteins. GRP78 may also interact with mutant and misfolded proteins, marking them for degredation. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C-terminus of GRP78 and other resident ER proteins including glucose regulated protein 94 (GRP94) and protein disulfide isomerase (PDI). The KDEL signal is recognized by the KDEL receptor and this interaction ensures recycling of escaped ER proteins back to the ER.

Database

Research Area

DATA IMAGES

Anti-Grp78 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22902

GTX22902 ICC/IF Image

ICC/IF analysis of MDCK cells using GTX22902 Grp78 antibody.
Green : Primary antibody
Blue : cell nucleus
Permeabilization : 0.1% Triton X-100
Dilution : 10 μg/ml in blocking buffer for at least 1 hour at room temperature

Anti-Grp78 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22902

GTX22902 ICC/IF Image

ICC/IF analysis of HMVEC Cells using GTX22902 Grp78 antibody.
Red : Primary antibody
Blue : Nuclei

Anti-Grp78 antibody used in Western Blot (WB). GTX22902

GTX22902 WB Image

WB analysis of rat liver lysate using GTX22902 Grp78 antibody.

Anti-Grp78 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22902

GTX22902 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX22902 Grp78 antibody.
Red : Primary antibody
Blue : Nuclei

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf