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Grp78 antibody

Anti-Grp78 antibody used in Flow cytometry (FACS). GTX48633
Anti-Grp78 antibody used in Western Blot (WB). GTX48633
Anti-Grp78 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48633
Anti-Grp78 antibody used in Flow cytometry (FACS). GTX48633
Anti-Grp78 antibody used in Western Blot (WB). GTX48633
Anti-Grp78 antibody used in Western Blot (WB). GTX48633

Cat No. GTX48633

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, FACS

Reactivity

Human, Mouse, Rat, Zebrafish, Sheep, Drosophila, Bovine, Chicken, Caenorhabditis elegans, Primate
Package
50 μl ($259)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 μg/ml
ICC/IF 1:50
FACS 1:150
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.02% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide made to an internal portion of human GRP78 (within residues 250-300). [Swiss-Prot# P11021]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_10619986

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 5 , BIP , GRP78 , HEL-S-89n , MIF2

Cellular Localization

Cytoplasm

Background

When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Grp78 antibody used in Flow cytometry (FACS). GTX48633

GTX48633 FACS Image

FACS (Intracellular staining) analysis of HeLa cells using GTX48633 Grp78 antibody.
Blue : Primary antibody
Orange : isotype control
Fixation : 4% PFA
Permibilization : 0.1% saponin

Anti-Grp78 antibody used in Western Blot (WB). GTX48633

GTX48633 WB Image

WB analysis of HeLa, A431, NIH-3T3, PC12 cell lysate using GTX48633 Grp78 antibody.
Dilution : 1 μg/ml

Anti-Grp78 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48633

GTX48633 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX48633 Grp78 antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 5 μg/ml

Anti-Grp78 antibody used in Flow cytometry (FACS). GTX48633

GTX48633 FACS Image

FACS (Intracellular staining) analysis of Jurkat cells using GTX48633 Grp78 antibody.
Blue : Primary antibody
Orange : isotype control
Dilution : 2 μg/mL
Fixation : 4% PFA
Permibilization : 0.1% saponin

Anti-Grp78 antibody used in Western Blot (WB). GTX48633

GTX48633 WB Image

WB analysis of HeLa cell lysate using GTX48633 Grp78 antibody.

Anti-Grp78 antibody used in Western Blot (WB). GTX48633

GTX48633 WB Image

WB analysis of HeLa cell lysate using GTX48633 Grp78 antibody.

REFERENCE

REVIEW

Grp78 antibody Cat No. GTX48633

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Dm
Figure Note : The data was peer reviewed and published in the journal Elife in 2016.PMID: 27669143
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Dm
Figure Note : The data was peer reviewed and published in the journal Elife in 2016.PMID: 27669143
SDS
PBS.pdf
Sodium Azide.pdf