GeneTex

  • Country / Location Selection

United States (US)

Grp78 antibody

Orthogonal
Anti-Grp78 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48633
Anti-Grp78 antibody used in Flow cytometry (FACS). GTX48633
Anti-Grp78 antibody used in Western Blot (WB). GTX48633
Anti-Grp78 antibody used in Flow cytometry (FACS). GTX48633
Anti-Grp78 antibody used in Western Blot (WB). GTX48633
Anti-Grp78 antibody used in Western Blot (WB). GTX48633
Anti-Grp78 antibody used in Western Blot (WB). GTX48633
Anti-Grp78 antibody used in Western Blot (WB). GTX48633

Cat. No. GTX48633

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Mouse, Rat, Zebrafish, Sheep, Drosophila, Bovine, Chicken, Caenorhabditis elegans, Primate
Package
50 μl ($399)
See all Grp78 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 μg/ml
ICC/IF 1:50
FACS 1:150
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide made to an internal portion of human GRP78 (within residues 250-300). [Swiss-Prot# P11021]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_10619986

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 5 , BIP , GRP78 , HEL-S-89n , MIF2

Cellular Localization

Cytoplasm

Background

When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Grp78 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48633

GTX48633 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX48633 Grp78 antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 5 μg/ml

Anti-Grp78 antibody used in Flow cytometry (FACS). GTX48633

GTX48633 FACS Image

FACS (Intracellular staining) analysis of Jurkat cells using GTX48633 Grp78 antibody.
Blue : Primary antibody
Orange : isotype control
Dilution : 2 μg/mL
Fixation : 4% PFA
Permibilization : 0.1% saponin

Anti-Grp78 antibody used in Western Blot (WB). GTX48633

GTX48633 WB Image

WB analysis of HeLa, A431, NIH-3T3, PC12 cell lysate using GTX48633 Grp78 antibody.
Dilution : 1 μg/ml

Anti-Grp78 antibody used in Flow cytometry (FACS). GTX48633

GTX48633 FACS Image

FACS (Intracellular staining) analysis of HeLa cells using GTX48633 Grp78 antibody.
Blue : Primary antibody
Orange : isotype control
Fixation : 4% PFA
Permibilization : 0.1% saponin

Anti-Grp78 antibody used in Western Blot (WB). GTX48633

GTX48633 WB Image

WB analysis of HeLa cell lysate using GTX48633 Grp78 antibody.

Anti-Grp78 antibody used in Western Blot (WB). GTX48633

GTX48633 WB Image

WB analysis of HeLa cell lysate using GTX48633 Grp78 antibody.

Anti-Grp78 antibody used in Western Blot (WB). GTX48633

GTX48633 WB Image

Orthogonal

The data was published in the journal Elife in 2016.PMID: 27669143

Anti-Grp78 antibody used in Western Blot (WB). GTX48633

GTX48633 WB Image

Orthogonal

The data was published in the journal Elife in 2016. PMID: 27669143

REFERENCE

Application Reference

Wu Z et al. Ecotoxicol Environ Saf 2021; 221 : 112438 Inhibition of endoplasmic reticulum stress-related autophagy attenuates MCLR-induced apoptosis in zebrafish testis and mouse TM4 cells.
Application : WB
Reactivity : Zebrafish
Sujuan Zhao et al. Environmental Pollution 2019; (Epub) Microcystin-LR exposure decreased the fetal weight of mice by disturbance of placental development and ROS-mediated endoplasmic reticulum stress in the placenta.
Application : WB
Vanhoutte D et al. Elife 2016; 5 (Epub) Thrombospondin expression in myofibers stabilizes muscle membranes.
Application : WB
Reactivity : Drosophila
Submit a Reference

REVIEW

There are currently no reviews for Grp78 antibody (GTX48633). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399