GeneTex
United States (US)

Guanine deaminase antibody

KO/KD
GTX115264_40275_20181102_WB_KO_watermark_19011519_938.jpg
GTX115264_40275_IHC_18121410_724.jpg
GTX115264_WB_18121410_644.jpg
GTX115264_40275_WB_R_colon_18121410_971.jpg
GTX115264_40275_WB_M_colon_18121410_393.jpg

Cat No. GTX115264

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

HeLa , HCT116

Predict Reactivity

Mouse, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.84 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Guanine deaminase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Guanine Deaminase,Cypin,Gah,Guanase,Nedasin,Gda

Background

The protein encoded by this gene is an enzyme that catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia. It is also known as a cytosolic regulator of PSD-95 postsynaptic targeting. [provided by RefSeq]

Database

DATA IMAGES
GTX115264_40275_20181102_WB_KO_watermark_19011519_938.jpg

GTX115264 WB Image

KO/KD

Wild-type (WT) and Guanine deaminase knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Guanine deaminase antibody (GTX115264) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX115264_40275_IHC_18121410_724.jpg

GTX115264 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Guanine deaminase(GTX115264) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115264_WB_18121410_644.jpg

GTX115264 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116
10% SDS PAGE
GTX115264 diluted at 1:1000

GTX115264_40275_WB_R_colon_18121410_971.jpg

GTX115264 WB Image

Guanine deaminase antibody detects Guanine deaminase protein by Western blot analysis.
A. 50 µg rat colon lysate/extract
10 % SDS-PAGE
Guanine deaminase antibody (GTX115264) dilution: 1:1000

GTX115264_40275_WB_M_colon_18121410_393.jpg

GTX115264 WB Image

Guanine deaminase antibody detects Guanine deaminase protein by Western blot analysis.
A. 50 µg mouse colon lysate/extract
10 % SDS-PAGE
Guanine deaminase antibody (GTX115264) dilution: 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169