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H2AFJ antibody

Anti-H2AFJ antibody used in Western Blot (WB). GTX53601

Cat No. GTX53601

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human
Package
400 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human H2AFJ.

Purification

Protein A purified, followed by peptide affinity purification.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

H2A histone family member J , H2AJ

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is located on chromosome 12 and encodes a replication-independent histone that is a variant H2A histone. The protein is divergent at the C-terminus compared to the consensus H2A histone family member. This gene also encodes an antimicrobial peptide with antibacterial and antifungal activity.[provided by RefSeq, Oct 2015]

Database

Research Area

DATA IMAGES

Anti-H2AFJ antibody used in Western Blot (WB). GTX53601

GTX53601 WB Image

WB analysis of Jurkat, MDA-MB435, MCF-7, HeLa cell lysate (35ug/lane) using GTX53601 H2AFJ antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf