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H6PD antibody [N2N3]

Anti-H6PD antibody [N2N3] used in Western Blot (WB). GTX101500
Anti-H6PD antibody [N2N3] used in Western Blot (WB). GTX101500
Anti-H6PD antibody [N2N3] used in IHC (Paraffin sections) (IHC-P). GTX101500

Cat. No. GTX101500

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all H6PD products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

89 kDa. ( Note )

Positive Control

HepG2 , Mouse liver

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.59 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human H6PD. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950476

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hexose-6-phosphate dehydrogenase/glucose 1-dehydrogenase , CORTRD1 , G6PDH , GDH

Cellular Localization

Endoplasmic reticulum lumen

Background

There are 2 forms of glucose-6-phosphate dehydrogenase. G form is X-linked and H form, encoded by this gene, is autosomally linked. This H form shows activity with other hexose-6-phosphates, especially galactose-6-phosphate, whereas the G form is specific for glucose-6-phosphate. Both forms are present in most tissues, but H form is not found in red cells. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-H6PD antibody [N2N3] used in Western Blot (WB). GTX101500

GTX101500 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse liver
7.5% SDS PAGE
GTX101500 diluted at 1:1000

Anti-H6PD antibody [N2N3] used in Western Blot (WB). GTX101500

GTX101500 WB Image

Sample(30 ug whole cell lysate)
A:Hep G2 (GTX27900)
7.5% SDS PAGE
GTX101500 diluted at 1:1000

Anti-H6PD antibody [N2N3] used in IHC (Paraffin sections) (IHC-P). GTX101500

GTX101500 IHC-P Image

H6PD antibody [N2N3] detects H6PD protein at cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
H6PD antibody [N2N3] (GTX101500) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169