GeneTex
United States (US)

H6PD antibody [N2N3]

GTX101500_WB_19011506_958.jpg
GTX101500_IHC_19011506_962.jpg
GTX101500_39911_WB_M_Liver_19011506_478.jpg

Cat No. GTX101500

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

89 kDa. ( Note )

Positive Control

H1299 , HepG2 , mouse liver

Predict Reactivity

Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.59mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human H6PD. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DKFZp686A01246 antibody, G6PDH antibody, GDH antibody, MGC87643 antibody, H6PD antibody, "glucose-6-phosphate dehydrogenase, salivary antibody", glucose 1- dehydrogenase antibody, 6-phosphogluconolactonase antibody, glucose dehydrogenase antibody, glucose dehyrogenase antibody, "G6PD, H form antibody", GDH/6PGL endoplasmic bifunctional protein antibody, hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase) antibody

Cellular Localization

Endoplasmic reticulum lumen

Background

There are 2 forms of glucose-6-phosphate dehydrogenase. G form is X-linked and H form, encoded by this gene, is autosomally linked. This H form shows activity with other hexose-6-phosphates, especially galactose-6-phosphate, whereas the G form is specific for glucose-6-phosphate. Both forms are present in most tissues, but H form is not found in red cells. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101500_WB_19011506_958.jpg

GTX101500 WB Image

Sample(30 ug whole cell lysate)
A:Hep G2 (GTX27900)
7.5% SDS PAGE
GTX101500 diluted at 1:1000

GTX101500_IHC_19011506_962.jpg

GTX101500 IHC-P Image

H6PD antibody [N2N3] detects H6PD protein at cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
H6PD antibody [N2N3] (GTX101500) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101500_39911_WB_M_Liver_19011506_478.jpg

GTX101500 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse liver
7.5% SDS PAGE
GTX101500 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169