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HA tag antibody [12CA5]

Anti-HA tag antibody [12CA5] used in Western Blot (WB). GTX16918
Anti-HA tag antibody [12CA5] used in Western Blot (WB). GTX16918

Cat. No. GTX16918

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 12CA5

Isotype

 IgG2b

Application

 WB, IP

Reactivity

 Species independent
Package
200 μl ($399)
See all HA tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IP Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Immunogen

KLH conjugated synthetic peptide encoding HA tag (YPYDVPDYA) was used as antigen.

Purification

Protein G purified, followed by dialysis against PBS.

Conjugation

Unconjugated

RRID

AB_371506

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-HA tag antibody [12CA5] used in Western Blot (WB). GTX16918

GTX16918 WB Image

WB analysis of HA-tag recombinant protein using GTX16918 HA tag antibody [12CA5].

Anti-HA tag antibody [12CA5] used in Western Blot (WB). GTX16918

GTX16918 WB Image

WB analysis of HA-tagged recombinant protein using GTX16918 HA tag antibody [12CA5].

REFERENCE

Application Reference

KI Fujita et al. Nat Commun 2024; 15 (1) : 455 Structural differences between the closely related RNA helicases, UAP56 and URH49, fashion distinct functional apo-complexes
Cepeda-Garcia C et al. Methods Mol Biol 2017; 1505 : 45-57 Determination of Cell Cycle Stage and Mitotic Exit Through the Quantification of the Protein Levels of Known Mitotic Regulators.
Roberts DN et al. Mol Cell 2006; 22 (5) : 633-44 Dephosphorylation and genome-wide association of Maf1 with Pol III-transcribed genes during repression.
Application : IP
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399