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HA tag antibody [GT134]

Anti-HA tag antibody [GT134] used in Immunoprecipitation (IP). GTX629540
Anti-HA tag antibody [GT134] used in Western Blot (WB). GTX629540

Cat. No. GTX629540

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT134

Isotype

 IgG2b

Application

 WB, IP

Reactivity

 Species independent
Package
100 μl ($399),
25 μl ($169)
See all HA tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IP 1:100-1:500
Not tested in other applications.

Positive Control

HA-tagged protein expressing HeLa cells

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to HA tag

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888120

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HA tag antibody , HA antibody , HA epitope tag antibody

Research Area

DATA IMAGES

Anti-HA tag antibody [GT134] used in Immunoprecipitation (IP). GTX629540

GTX629540 IP Image

HA tag antibody [GT134] immunoprecipitates HA tagged protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 20 μg whole cell lysate/extract of HA tagged protein expressing 293T cells B : Control with 2.5 μg of pre-immune mouse IgG C : Immunoprecipitation of HA tagged protein by 2.5 μg of HA tag antibody [GT134] (GTX629540) 10% SDS-PAGE The immunoprecipitated HA tagged protein was detected by HA tag antibody [GT134] (GTX629540) diluted at 1 : 3000. EasyBlot anti-mouse IgG (HRP) (GTX221667-01) was used as a secondary reagent.

Anti-HA tag antibody [GT134] used in Western Blot (WB). GTX629540

GTX629540 WB Image

HA tag antibody [GT134] detects HA tag protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
B. 30 μg whole cell lysate/extract of HA tagged protein expressing HeLa cells
7.5 % SDS-PAGE
HA tag antibody [GT134] (GTX629540) dilution: 1:5000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations