HA tag antibody [GT423]

HA tag antibody [GT423] detects HA tag protein by western blot analysis. HA tag-transfected and GST tag-transfected (30 μg) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with HA tag antibody [GT423] (GTX628489) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HA tag antibody [GT423] (GTX628489) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

HA tag antibody [GT423] immunoprecipitates HA-HIF-1α protein in IP experiments.
IP Sample: 400 μg cell lysate/extract of HA-HIF-1α transfected 293T cells
A. Control with 2 μg of preimmune mouse IgG
B. Immunoprecipitation of HA-fused protein by 2 μg of HA tag antibody [GT423] (GTX628489)
5% SDS-PAGE
The immunoprecipitated HA-HIF1α protein was detected by HIF-1α antibody (GTX127309) diluted at 1:1000.

HA tag antibody [GT423] detects HA protein by immunofluorescent analysis.
Sample: HA-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HA stained by HA tag antibody [GT423] (GTX628489) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with HA tag antibody [GT423] (GTX628489) diluted at 1:5000 and competitor's antibody (sc-7392) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

The data was published in the journal Sci Rep in 2017.PMID: 28676638

The data was published in the journal Sci Rep in 2015. PMID: 25740706

The data was published in the journal Sci Rep in 2015. PMID: 25740706

The data was published in the journal BMC Mol Cell Biol in 2020.PMID: 32384871

The data was published in the journal Sci Rep in 2017.PMID: 28676638