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HA tag antibody [GT423]

Anti-HA tag antibody [GT423] used in Western Blot (WB). GTX628489
Anti-HA tag antibody [GT423] used in Western Blot (WB). GTX628489
Anti-HA tag antibody [GT423] used in Immunoprecipitation (IP). GTX628489
Anti-HA tag antibody [GT423] used in Western Blot (WB). GTX628489
Anti-HA tag antibody [GT423] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628489

Cat No. GTX628489

Host

Mouse

Clonality

Monoclonal

Clone Name

GT423

Isotype

IgG2b

Application

WB, ICC/IF, IP
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:2000
IP 1:100-1:500
Not tested in other applications.

Positive Control

HeLa , HA-tagged protein expressing HeLa

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to HA tag

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888042

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

HA tag antibody , HA antibody , HA epitope tag antibody

Research Area

DATA IMAGES

Anti-HA tag antibody [GT423] used in Western Blot (WB). GTX628489

GTX628489 WB Image

HA tag antibody [GT423] detects HA tag protein by western blot analysis. HA tag-transfected and GST tag-transfected (30 μg) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with HA tag antibody [GT423] (GTX628489) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-HA tag antibody [GT423] used in Western Blot (WB). GTX628489

GTX628489 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HA tag antibody [GT423] (GTX628489) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-HA tag antibody [GT423] used in Immunoprecipitation (IP). GTX628489

GTX628489 IP Image

HA tag antibody [GT423] immunoprecipitates HA-HIF-1α protein in IP experiments.
IP Sample: 400 μg cell lysate/extract of HA-HIF-1α transfected 293T cells
A. Control with 2 μg of preimmune mouse IgG
B. Immunoprecipitation of HA-fused protein by 2 μg of HA tag antibody [GT423] (GTX628489)
5% SDS-PAGE
The immunoprecipitated HA-HIF1α protein was detected by HIF-1α antibody (GTX127309) diluted at 1:1000.

Anti-HA tag antibody [GT423] used in Western Blot (WB). GTX628489

GTX628489 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with HA tag antibody [GT423] (GTX628489) diluted at 1:5000 and competitor's antibody (sc-7392) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-HA tag antibody [GT423] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628489

GTX628489 ICC/IF Image

HA tag antibody [GT423] detects HA protein by immunofluorescent analysis.
Sample: HA-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HA stained by HA tag antibody [GT423] (GTX628489) diluted at 1:2000.
Blue: Hoechst 33342 staining.

REFERENCE

REVIEW

HA tag antibody [GT423] Cat No. GTX628489

Rating ( Average 4.7 based on 6 users reviews)
Application
Western Blot(WB) ( Average 4.6 based on 5 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Immunoprecipitation(IP) ( Average 5 based on 3 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IP,WB
Figure Note : The data was peer reviewed and published in the journal BMC Mol Cell Biol in 2020.PMID: 32384871
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28676638
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IP,WB
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28676638
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB,IP
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2015.PMID: 25740706
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2015.PMID: 25740706
Date : Anonymous submitted on 02-Jul-2014
Score :
Application Tested : WB
Sample : HA-tagged protein expressing COS7 cells
Amount used : 100μg
Blocking : skim milk, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Lane Description : Lane A,B:HA-tagged protein expressing COS7 cells
Application Reference
SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169