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HA tag antibody [GT423]

Comparison Overexpression
Anti-HA tag antibody [GT423] used in Western Blot (WB). GTX628489
Anti-HA tag antibody [GT423] used in Western Blot (WB). GTX628489
Anti-HA tag antibody [GT423] used in Immunoprecipitation (IP). GTX628489
Anti-HA tag antibody [GT423] used in Western Blot (WB). GTX628489
Anti-HA tag antibody [GT423] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628489

Cat. No. GTX628489

Reference 10
6

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT423

Isotype

 IgG2b

Application

 WB, ICC/IF, IP
Package
100 μl ($399),
25 μl ($169)
See all HA tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:2000
IP 1:100-1:500
Not tested in other applications.

Positive Control

HA-tag fusion protein-transfected 293T cells

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to HA tag

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888042

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

HA tag antibody , HA antibody , HA epitope tag antibody

Research Area

DATA IMAGES

Anti-HA tag antibody [GT423] used in Western Blot (WB). GTX628489

GTX628489 WB Image

HA tag antibody [GT423] detects HA tag protein by western blot analysis. HA tag-transfected and GST tag-transfected (30 μg) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with HA tag antibody [GT423] (GTX628489) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-HA tag antibody [GT423] used in Western Blot (WB). GTX628489

GTX628489 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HA tag antibody [GT423] (GTX628489) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-HA tag antibody [GT423] used in Immunoprecipitation (IP). GTX628489

GTX628489 IP Image

HA tag antibody [GT423] immunoprecipitates HA-HIF-1α protein in IP experiments.
IP Sample: 400 μg cell lysate/extract of HA-HIF-1α transfected 293T cells
A. Control with 2 μg of preimmune mouse IgG
B. Immunoprecipitation of HA-fused protein by 2 μg of HA tag antibody [GT423] (GTX628489)
5% SDS-PAGE
The immunoprecipitated HA-HIF1α protein was detected by HIF-1α antibody (GTX127309) diluted at 1:1000.

Anti-HA tag antibody [GT423] used in Western Blot (WB). GTX628489

GTX628489 WB Image

Comparison

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with HA tag antibody [GT423] (GTX628489) diluted at 1:5000 and competitor's antibody (sc-7392) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-HA tag antibody [GT423] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628489

GTX628489 ICC/IF Image

HA tag antibody [GT423] detects HA protein by immunofluorescent analysis.
Sample: HA-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HA stained by HA tag antibody [GT423] (GTX628489) diluted at 1:2000.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Hernandez JC et al. iScience 2023; 26 (3) : 106254 LIN28 and histone H3K4 methylase induce TLR4 to generate tumor-initiating stem-like cells.
Horv?th A et al. BMC Mol Cell Biol 2020; 21 (1) : 36 Interaction between NSMCE4A and GPS1 links the SMC5/6 complex to the COP9 signalosome.
Li J et al. Biochem Biophys Res Commun 2020; 528 (2) : 276-284 NLS-RARα blocks cell differentiation by inhibiting the retinoic acid signalling pathway.
Application : WB, ICC/IF, IP
Osada Y et al. Biochim Biophys Acta Gen Subj 2020; 1864 (9) : 129637 The fibrinogen C-terminal domain is seldom C-mannosylated but its C-mannosylation is important for the secretion of microfibril-associated glycoprotein 4.
Application : WB
Rao VK et al. J Cell Physiol 2019; 234 (6) : 8683-8697 A pH-sensitive luminal His-cluster promotes interaction of PAM with V-ATPase along the secretory and endocytic pathways of peptidergic cells.
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Submit a Reference

REVIEW

HA tag antibody [GT423] Cat. No. GTX628489

Rating
( Average 4.7 based on 6 users reviews)
Application
Western Blot(WB)
( Average 4.6 based on 5 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Immunoprecipitation(IP)
( Average 5 based on 3 users reviews)
Anti-HA tag antibody [GT423] used in Western Blot and Immunoprecipitation (WB IP). GTX628489
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IP,WB
Figure Note : The data was peer reviewed and published in the journal BMC Mol Cell Biol in 2020.PMID: 32384871
Anti-HA tag antibody [GT423] used in Western Blot (WB). GTX628489
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28676638
Anti-HA tag antibody [GT423] used in Western Blot and Immunoprecipitation (WB IP). GTX628489
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IP,WB
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28676638
Anti-HA tag antibody [GT423] used in Western Blot and Immunoprecipitation (WB IP). GTX628489
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB,IP
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2015. PMID: 25740706
Anti-HA tag antibody [GT423] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628489
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2015. PMID: 25740706
Anti-HA tag antibody [GT423] used in Western Blot (WB). GTX628489
Date : Anonymous submitted on 02-Jul-2014
Score :
Application Tested : WB
Sample : HA-tagged protein expressing COS7 cells
Amount used : 100μg
Blocking : skim milk, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Lane Description : Lane A,B:HA-tagged protein expressing COS7 cells
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Submit a Review
Application Reference
Hernandez JC et al. iScience 2023; 26 (3):106254 LIN28 and histone H3K4 methylase induce TLR4 to generate tumor-initiating stem-like cells.
Horv?th A et al. BMC Mol Cell Biol 2020; 21 (1):36 Interaction between NSMCE4A and GPS1 links the SMC5/6 complex to the COP9 signalosome.
Li J et al. Biochem Biophys Res Commun 2020; 528 (2):276-284 NLS-RARα blocks cell differentiation by inhibiting the retinoic acid signalling pathway.
Application : WB, ICC/IF, IP
Osada Y et al. Biochim Biophys Acta Gen Subj 2020; 1864 (9):129637 The fibrinogen C-terminal domain is seldom C-mannosylated but its C-mannosylation is important for the secretion of microfibril-associated glycoprotein 4.
Application : WB
Rao VK et al. J Cell Physiol 2019; 234 (6):8683-8697 A pH-sensitive luminal His-cluster promotes interaction of PAM with V-ATPase along the secretory and endocytic pathways of peptidergic cells.
Liu CS et al. Sci Rep 2017; 7 (1):4613 Cbl-mediated K63-linked ubiquitination of JAK2 enhances JAK2 phosphorylation and signal transduction.
Application : WB
Kakisaka M et al. Virology 2016; 496:116-126 Intrinsically disordered region of influenza A NP regulates viral genome packaging via interactions with viral RNA and host PI(4,5)P2.
Application : WB
Liu Y et al. Sci Rep 2015; 5:8796 FOXK2 transcription factor suppresses ERα-positive breast cancer cell growth through down-regulating the stability of ERα via mechanism involving BRCA1/BARD1.
Application : WB, IP
Cui XA et al. J Cell Sci 2015; 128 (18):3398-410 mRNA encoding Sec61β, a tail-anchored protein, is localized on the endoplasmic reticulum.
Application : WB
Chodaparambil JV et al. EMBO J 2014; 33 (7):719-31 Molecular functions of the TLE tetramerization domain in Wnt target gene repression.
Application : WB
SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations