*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
HeLa cell expressing HA-tagged protein
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
The immunogen used to generate this antibody corresponds to HA tag
Affinity purified by Protein G.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
HA tag antibody , HA antibody , HA epitope tag antibody
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with HA tag antibody [GT4810] (GTX628902) diluted at 1:5000 and competitor's antibody (sc-7392) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX628902 WB Image
HA tag antibody [GT4810] detects HA tag protein by western blot analysis. A. 30 μg HeLa whole cell lysate/extract B. 30 μg whole cell lysate/extract of HA tagged protein expressing HeLa cells 5% SDS-PAGE HA tag antibody [GT4810] (GTX628902) dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628902 IP Image
HA tag antibody [GT4810] immunoprecipitates HA tagged protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 20 μg whole cell lysate/extract of HA tagged protein expressing 293T cells B : Control with 2.5 μg of pre-immune mouse IgG C : Immunoprecipitation of HA tagged protein by 2.5 μg of HA tagg antibody (GTX628902) 10% SDS-PAGE The immunoprecipitated HA tagged protein was detected by HA tag antibody [GT4810] (GTX628902) diluted at 1 : 3000. EasyBlot anti-mouse IgG (HRP) (GTX221667-01) was used as a secondary reagent.
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GTX628902 ICC/IF Image
HA tag antibody [GT4810] detects HA protein by immunofluorescent analysis.Sample: HA-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HA stained by HA tag antibody [GT4810] (GTX628902) diluted at 1:1000.Blue: Hoechst 33342 staining.