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HA tag antibody [GT4810]

Comparison
Anti-HA tag antibody [GT4810] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628902
Anti-HA tag antibody [GT4810] used in Immunoprecipitation (IP). GTX628902
Anti-HA tag antibody [GT4810] used in Western Blot (WB). GTX628902
Anti-HA tag antibody [GT4810] used in Western Blot (WB). GTX628902

Cat. No. GTX628902

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT4810

Isotype

 IgG2a

Application

 WB, ICC/IF, IP

Reactivity

 Species independent
Package
100 μl ($399),
25 μl ($169)
See all HA tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Positive Control

HeLa cell expressing HA-tagged protein

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to HA tag

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888078

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HA tag antibody , HA antibody , HA epitope tag antibody

Research Area

DATA IMAGES

Anti-HA tag antibody [GT4810] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628902

GTX628902 ICC/IF Image

HA tag antibody [GT4810] detects HA protein by immunofluorescent analysis.
Sample: HA-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HA stained by HA tag antibody [GT4810] (GTX628902) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Anti-HA tag antibody [GT4810] used in Immunoprecipitation (IP). GTX628902

GTX628902 IP Image

HA tag antibody [GT4810] immunoprecipitates HA tagged protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 20 μg whole cell lysate/extract of HA tagged protein expressing 293T cells B : Control with 2.5 μg of pre-immune mouse IgG C : Immunoprecipitation of HA tagged protein by 2.5 μg of HA tagg antibody (GTX628902) 10% SDS-PAGE The immunoprecipitated HA tagged protein was detected by HA tag antibody [GT4810] (GTX628902) diluted at 1 : 3000. EasyBlot anti-mouse IgG (HRP) (GTX221667-01) was used as a secondary reagent.

Anti-HA tag antibody [GT4810] used in Western Blot (WB). GTX628902

GTX628902 WB Image

HA tag antibody [GT4810] detects HA tag protein by western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
B. 30 μg whole cell lysate/extract of HA tagged protein expressing HeLa cells
5% SDS-PAGE
HA tag antibody [GT4810] (GTX628902) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-HA tag antibody [GT4810] used in Western Blot (WB). GTX628902

GTX628902 WB Image

Comparison

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with HA tag antibody [GT4810] (GTX628902) diluted at 1:5000 and competitor's antibody (sc-7392) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

REFERENCE

Application Reference

Ramanantsalama MR et al. J Cell Sci 2022; 135 (11) : jcs259893 TFK1, a basal body transition fibre protein that is essential for cytokinesis in Trypanosoma brucei
Application : ICC/IF
Charlotte Isch et al. PLoS Pathog 2021; 17 (8) : e1009329 Structural and functional studies of the first tripartite protein complex at the Trypanosoma brucei flagellar pocket collar.
Application : ICC/IF
Isch C. et al. bioRxiv 2021; (Epub) Structural and functional studies of the first tripartite protein complex at the Trypanosoma brucei flagellar pocket collar.
Application : ICC/IF
Submit a Reference

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations