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HA tag antibody [HA.C5]

Anti-HA tag antibody [HA.C5] used in Western Blot (WB). GTX18181

Cat. No. GTX18181

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 HA.C5

Isotype

 IgG3

Application

 WB, ICC/IF, IP, ELISA, IHC
Package
100 μg ($399)
See all HA tag products

APPLICATION

Application Note

Recommended Starting Dilutions:
WB (with ECL): Use at a dilution of 1:1000-3000
Immunostaining: Use at a dilution of 1:500 - 1:2000

For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test

Product Note

Recognizes the N-terminal, C-terminal or internal HA-tagged fusion proteins

PROPERTIES

Form

Liquid

Buffer

10mM PBS pH7.2

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

YPYDVPDYA (HA) synthetic peptide conjugated to KLH

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_423116

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

HA epitope tag

Background

The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been extensively used as a general epitope tag in expression vectors.

Research Area

DATA IMAGES

Anti-HA tag antibody [HA.C5] used in Western Blot (WB). GTX18181

GTX18181 WB Image

1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with HA-tagged protein vector; untransfected (1) and transfected (2)

REFERENCE

Application Reference

Myler LR et al. Mol Cell 2017; 67 (5) : 891-898.e4 Single-Molecule Imaging Reveals How Mre11-Rad50-Nbs1 Initiates DNA Break Repair.
Application : WB
Rivera-Serrano EE et al. J Virol 2017; 91 (7) A Cytoplasmic RNA Virus Alters the Function of the Cell Splicing Protein SRSF2.
Application : ICC/IF
Irie T et al. Virology 2008; 371 (1) : 108-20 Recruitment of Alix/AIP1 to the plasma membrane by Sendai virus C protein facilitates budding of virus-like particles.
Application : WB
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SDS
PBS.pdf
Package List Price ($)
$ 399