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HA tag antibody [HA.C5]

Anti-HA tag antibody [HA.C5] used in Western Blot (WB). GTX18181

Cat No. GTX18181

Host

Mouse

Clonality

Monoclonal

Clone Name

HA.C5

Isotype

IgG3

Application

WB, ICC/IF, IP, ELISA, IHC
Package
100 μg ($339)

APPLICATION

Application Note

Recommended Starting Dilutions:
WB (with ECL): Use at a dilution of 1:1000-3000
Immunostaining: Use at a dilution of 1:500 - 1:2000

For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test

Product Note

Recognizes the N-terminal, C-terminal or internal HA-tagged fusion proteins

PROPERTIES

Form

Liquid

Buffer

10mM PBS, pH 7.2, 0.05% NaN3

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

YPYDVPDYA (HA) synthetic peptide conjugated to KLH

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_423116

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

HA epitope tag

Background

The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been extensively used as a general epitope tag in expression vectors.

Research Area

DATA IMAGES

Anti-HA tag antibody [HA.C5] used in Western Blot (WB). GTX18181

GTX18181 WB Image

1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with HA-tagged protein vector; untransfected (1) and transfected (2)

REFERENCE

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SDS
PBS.pdf
Package List Price ($)
$ 339