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HA tag antibody [HL1603]

Recombinant antibody
Anti-HA tag antibody [HL1603] used in ELISA (ELISA). GTX637082
Anti-HA tag antibody [HL1603] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637082
Anti-HA tag antibody [HL1603] used in Western Blot (WB). GTX637082
Anti-HA tag antibody [HL1603] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX637082

Cat No. GTX637082

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1603

Isotype

IgG

Application

WB, ICC/IF, ELISA, IHC-P (cell pellet)
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
ELISA Assay dependent
IHC-P (cell pellet) Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to HA tag.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-HA tag antibody [HL1603] used in ELISA (ELISA). GTX637082

GTX637082 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant HA-tag tagged protein (1000-15.63 ng/mL). Coated protein was probed with HA tag antibody [HL1603] (GTX637082) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Anti-HA tag antibody [HL1603] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637082

GTX637082 ICC/IF Image

HA tag antibody [HL1603] detects HA tag protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HA tag stained by HA tag antibody [HL1603] (GTX637082) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-HA tag antibody [HL1603] used in Western Blot (WB). GTX637082

GTX637082 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with 6X His tag antibody [HL1603] (GTX637082) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HA tag antibody [HL1603] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX637082

GTX637082 IHC-P (cell pellet) Image

HA tag antibody [HL1603] detects HA tag protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mock and HA tag - transfected 293T cell pellet.
Green: HA tag stained by HA tag antibody [HL1603] (GTX637082) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 379
$ 169
Custom Conjugations