GeneTex
United States (US)

HA tag antibody (HRP)

GTX115044-01_40421_20150529_ELISA_18121410_226.jpg
GTX115044-01_42731_20170209_WB_HA-NRAS_18121410_634.jpg

Cat No. GTX115044-01

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ELISA
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ELISA 1:1000-1:10000
Not tested in other applications.

Positive Control

293T , HA-tagged NRAS
PROPERTIES

Form

Liquid

Buffer

0.05M Tris, 0.15M NaCl (pH7.4), 1%BSA. 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.85 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Rabbits were immunized with YPYDVPDYA conjugated with KLH.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Horseradish peroxidase(HRP)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HA tag antibody, HA antibody, HA epitope tag antibody

Research Area

DATA IMAGES
GTX115044-01_40421_20150529_ELISA_18121410_226.jpg

GTX115044-01 ELISA Image

An ELISA plate is coated with 50 μL of a DDDDK Tag conjugated BSA at concentration of 5 μg/mL. The peptide conjugated carrier protein is coated and detected with HRP-conjugated anti-DDDDK Tag antibody (GTX115044-01) at concentration ranged from 3.1 to 200 ng/mL.

GTX115044-01_42731_20170209_WB_HA-NRAS_18121410_634.jpg

GTX115044-01 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with HA Tag antibody (HRP) (GTX115044-01) diluted at 1:3000.

REFERENCE
SDS
Proclin.pdf
Package List Price ($)
$ 319
$ 169