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HA tag antibody (HRP)

Anti-HA tag antibody (HRP) used in Western Blot (WB). GTX115044-01
Anti-HA tag antibody (HRP) used in ELISA (ELISA). GTX115044-01

Cat. No. GTX115044-01

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA
Package
100 μl ($399),
25 μl ($169)
See all HA tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ELISA 1:1000-1:10000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.32 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Rabbits were immunized with YPYDVPDYA conjugated with KLH.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Horseradish peroxidase(HRP)

RRID

AB_11169023

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

HA tag antibody , HA antibody , HA epitope tag antibody

Research Area

DATA IMAGES

Anti-HA tag antibody (HRP) used in Western Blot (WB). GTX115044-01

GTX115044-01 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HA tag antibody (HRP) (GTX115044-01) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HA tag antibody (HRP) used in ELISA (ELISA). GTX115044-01

GTX115044-01 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant HA-tag tagged protein (1000-15.63 ng/mL). Coated protein was probed with HA tag antibody (HRP) (GTX115044-01) (1 μg/mL).

REFERENCE

Application Reference

Emmett MJ et al. Nature 2017; (Epub) Histone deacetylase 3 prepares brown adipose tissue for acute thermogenic challenge.
Application : WB
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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 399
$ 169