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HA tag antibody (HRP)

Anti-HA tag antibody (HRP) used in Western Blot (WB). GTX77400

Cat No. GTX77400

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA
Package
100 μl ($319)

APPLICATION

Application Note

Suggested starting dilutions are as follows: WB: 1:500-1:3000, ELISA. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Positive Control

HA-NRAS-tranfected 293T

PROPERTIES

Form

Liquid

Buffer

0.05M Tris, 0.15M NaCl (pH7.4), 1%BSA. 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rabbit

Immunogen

Rabbits were immunized with HA-tag conjugated to KLH. After multiple immunizations in Freund's adjuvant, serum was collected. Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support then conjugated with HRP ( Horseradish Peroxidase).

Purification

Purified by antigen-affinity chromatography.

Conjugation

Horseradish peroxidase(HRP)

RRID

AB_379994

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-HA tag antibody (HRP) used in Western Blot (WB). GTX77400

GTX77400 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HA Tag antibody (HRP) (GTX77400) diluted at 1:3000.

REFERENCE

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SDS
Proclin.pdf
Package List Price ($)
$ 319