GeneTex
  • Country / Location Selection

United States (US)

HA tag antibody

Anti-HA tag antibody used in Western Blot (WB). GTX115044
Anti-HA tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115044
Anti-HA tag antibody used in ELISA (ELISA). GTX115044
Anti-HA tag antibody used in Western Blot (WB). GTX115044
Anti-HA tag antibody used in Immunoprecipitation (IP). GTX115044

Cat No. GTX115044

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP, ELISA
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IP 1:100-1:500
ELISA 1:1000-1:10000
Not tested in other applications.

Positive Control

HA-tagged protein expressing 293T

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to HA tag

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10622369

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

HA tag antibody , HA antibody , HA epitope tag antibody

Research Area

DATA IMAGES

Anti-HA tag antibody used in Western Blot (WB). GTX115044

GTX115044 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HA tag antibody (GTX115044) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HA tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115044

GTX115044 ICC/IF Image

Immunofluorescence analysis of HA-recombinant protein transfected HeLa cell which fixed with paraformaldehyde, using HA tag(GTX115044) antibody at 1:500 dilution.

Anti-HA tag antibody used in ELISA (ELISA). GTX115044

GTX115044 ELISA Image

An ELISA plate is coated with 50 μL of a DDDDK Tag conjugated BSA at concentration of 5 μg/mL. The peptide conjugated carrier protein is coated and detected with anti-DDDDK Tag antibody (GTX115044) at concentration ranged from 3.1 to 200 ng/mL.

Anti-HA tag antibody used in Western Blot (WB). GTX115044

GTX115044 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HA tag antibody (GTX115044) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HA tag antibody used in Immunoprecipitation (IP). GTX115044

GTX115044 IP Image

Immunoprecipitation of HA tag protein from 293T whole cell extracts using 5 μg of HA tag antibody (GTX115044).
Western blot analysis was performed using HA tag antibody (GTX115044) diluted at 1:2500.
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01) was used to detect the primary antibody.

REFERENCE

REVIEW

HA tag antibody Cat No. GTX115044

Rating ( Average 5 based on 3 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 23-Dec-2020
Score :
Application Tested : WB
Figure Note : The data was pear reviewed and published in the journal Front Microbiol in 2019.PMID: 31969874
Date : Anonymous submitted on 27-Oct-2016
Score :
Application Tested : WB
Sample : HA-human ApoA1-transfected HeLa cells
Amount used : 15μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:20000, 4°C, 16Hr
Date : Anonymous submitted on 27-Oct-2016
Score :
Application Tested : ICC/IF
Sample : HA-human ApoA1-transfected HeLa cells
Blocking : 3% BSA, 25°C, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.05% saponin
Primary Antibody : 1:2000, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169