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HA tag antibody

Anti-HA tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115044
Anti-HA tag antibody used in ELISA (ELISA). GTX115044
Anti-HA tag antibody used in Western Blot (WB). GTX115044
Anti-HA tag antibody used in Immunoprecipitation (IP). GTX115044

Cat. No. GTX115044

Reference 47
7

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP, ELISA
Package
100 μl ($399),
25 μl ($169)
See all HA tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IP 1:100-1:500
ELISA 1:1000-1:10000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to HA tag

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10622369

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

HA tag antibody , HA antibody , HA epitope tag antibody

Research Area

DATA IMAGES

Anti-HA tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115044

GTX115044 ICC/IF Image

Immunofluorescence analysis of HA-recombinant protein transfected HeLa cell which fixed with paraformaldehyde, using HA tag(GTX115044) antibody at 1:500 dilution.

Anti-HA tag antibody used in ELISA (ELISA). GTX115044

GTX115044 ELISA Image

An ELISA plate is coated with 50 μL of a HA-Tag conjugated BSA at concentration of 5 μg/mL. The peptide conjugated carrier protein is coated and detected with anti-HA tag antibody (GTX115044) at concentration ranged from 3.1 to 200 ng/mL.

Anti-HA tag antibody used in Western Blot (WB). GTX115044

GTX115044 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HA tag antibody (GTX115044) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HA tag antibody used in Immunoprecipitation (IP). GTX115044

GTX115044 IP Image

Immunoprecipitation of HA tag protein from 293T whole cell extracts using 5 μg of HA tag antibody (GTX115044).
Western blot analysis was performed using HA tag antibody (GTX115044) diluted at 1:2500.
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Y Li et al. Cell Death Dis 2023; KLF12 promotes the proliferation of breast cancer cells by reducing the transcription of p21 in a p53-dependent and p53-independent manner
Application : WB, IP
Xia K et al. Cell Death Dis 2023; 14 (4) : 250 Cryptochrome 2 acetylation attenuates its antiproliferative effect in breast cancer.
Application : WB
Lin CY et al. Sci Adv 2023; 9 (14) : eade9944 Small-molecule PIK-93 modulates the tumor microenvironment to improve immune checkpoint blockade response.
Application : WB, IP
Alvim JC et al. Nat Commun 2023; 14 (1) : 1612 The K/HDEL receptor does not recycle but instead acts as a Golgi-gatekeeper.
Application : IP
Takemoto R et al. J Virol 2022; 96 (3) : e0194921 Short-Stalk Isoforms of CADM1 and CADM2 Trigger Neuropathogenic Measles Virus-Mediated Membrane Fusion by Interacting with the Viral Hemagglutinin.
Show More +
Submit a Reference

REVIEW

HA tag antibody Cat. No. GTX115044

Rating
( Average 5 based on 7 users reviews)
Application
Western Blot(WB)
( Average 5 based on 6 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-HA tag antibody used in Western Blot (WB). GTX115044
Date : Chen-I Hsu submitted on 16-Sep-2021
Score :
Application Tested : WB
Sample : A549 cells
Amount used : 50μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:2000, 25°C, 1Hr
Secondary Antibody : GTX213110-01, 1:2000, 25°C, 1Hr
Lane Description : The A549 cells were transfected with HA-tagged DNAJB6 cDNA and harvested at 24h post-transfection. The DNAJB6 antibody recognized both endogenous and HA-tagged DNAJB6 while the Genetex HA-tag antibody only recognized one band.
Anti-HA tag antibody used in Western Blot (WB). GTX115044
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2016.PMID: 26783207
Anti-HA tag antibody used in Western Blot (WB). GTX115044
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2016.PMID: 26783207
Anti-HA tag antibody used in Western Blot (WB). GTX115044
Date : Anonymous submitted on 06-May-2021
Score :
Application Tested : WB
Sample : A549 cells
Amount used : 10 μg
Blocking : 5% skim milk in TBST, 27°C, 1Hr
Primary Antibody : 1:5000, 4°C, 12Hr
Secondary Antibody : GTX213110-01, 1:5000, 27°C, 2Hr
Lane Description : Two groups (HA protein -/+) of whole cell extracts (10 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HA antibody (GTX115044) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Anti-HA tag antibody used in Western Blot (WB). GTX115044
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Front Microbiol in 2019. PMID: 31969874
Anti-HA tag antibody used in Western Blot (WB). GTX115044
Date : Anonymous submitted on 27-Oct-2016
Score :
Application Tested : WB
Sample : HA-human ApoA1-transfected HeLa cells
Amount used : 15μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:20000, 4°C, 16Hr
Anti-HA tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115044
Date : Anonymous submitted on 27-Oct-2016
Score :
Application Tested : ICC/IF
Sample : HA-human ApoA1-transfected HeLa cells
Blocking : 3% BSA, 25°C, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.05% saponin
Primary Antibody : 1:2000, 4°C, 16Hr
Show More +
Submit a Review
Application Reference
Y Li et al. Cell Death Dis 2023; KLF12 promotes the proliferation of breast cancer cells by reducing the transcription of p21 in a p53-dependent and p53-independent manner
Application : WB, IP
Xia K et al. Cell Death Dis 2023; 14 (4):250 Cryptochrome 2 acetylation attenuates its antiproliferative effect in breast cancer.
Application : WB
Lin CY et al. Sci Adv 2023; 9 (14):eade9944 Small-molecule PIK-93 modulates the tumor microenvironment to improve immune checkpoint blockade response.
Application : WB, IP
Alvim JC et al. Nat Commun 2023; 14 (1):1612 The K/HDEL receptor does not recycle but instead acts as a Golgi-gatekeeper.
Application : IP
Chang CK et al. J Fungi (Basel) 2022; 8 (11) The Role of Sfp1 in Candida albicans Cell Wall Maintenance.
Takemoto R et al. J Virol 2022; 96 (3):e0194921 Short-Stalk Isoforms of CADM1 and CADM2 Trigger Neuropathogenic Measles Virus-Mediated Membrane Fusion by Interacting with the Viral Hemagglutinin.
Domenico Azarnia Tehran et al. Sci Adv 2022; 8 (21):eabl5032 Selective endocytosis of Ca2+-permeable AMPARs by the Alzheimer's disease risk factor CALM bidirectionally controls synaptic plasticity.
Anshu Agarwal et al. Microbiol Spectr 2022; 10 (3):e0083022 Japanese Encephalitis Virus NS4A Protein Interacts with PTEN-Induced Kinase 1 (PINK1) and Promotes Mitophagy in Infected Cells.
Application : ICC/IF
Christine E Broster Reix et al. Microbiol Spectr 2021; 9 (2):e0091521 Intrabody-Induced Cell Death by Targeting the T. brucei Cytoskeletal Protein TbBILBO1.
Application : ICC/IF
Aman S et al. Cell Death Discov 2021; 7 (1):351 DACH1 inhibits breast cancer cell invasion and metastasis by down-regulating the transcription of matrix metalloproteinase 9.
Application : WB
Chen WT et al. J Biomed Sci 2021; 28 (1):81 Elp1 facilitates RAD51-mediated homologous recombination repair via translational regulation.
Application : WB
Yang Y et al. Cell Death Discov 2021; 7 (1):328 FBXL10 promotes EMT and metastasis of breast cancer cells via regulating the acetylation and transcriptional activity of SNAI1.
Li X et al. NPJ Precis Oncol 2021; 5 (1):20 Acetylation of ELF5 suppresses breast cancer progression by promoting its degradation and targeting CCND1.
Application : WB, IP
Lee SA et al. Viruses 2021; 13 (3) Non-Metabolic Functions of PKM2 Contribute to Cervical Cancer Cell Proliferation Induced by the HPV16 E7 Oncoprotein.
Application : WB
Tsai MH et al. Acta Neuropathol Commun 2020; 8 (1):106 Impairment in dynein-mediated nuclear translocation by BICD2 C-terminal truncation leads to neuronal migration defect and human brain malformation.
Seo J et al. Commun Biol 2020; 3 (1):638 Fatty-acid-induced FABP5/HIF-1 reprograms lipid metabolism and enhances the proliferation of liver cancer cells.
Yao Z et al. Nat Commun 2020; 11 (1):2440 Split Intein-Mediated Protein Ligation for detecting protein-protein interactions and their inhibition.
Su YC et al. FASEB J 2020; 34 (6):7283-7294 MicroRNA-155 inhibits dengue virus replication by inducing heme oxygenase-1-mediated antiviral interferon responses.
Application : WB
Constantino-Jonapa LA et al. Parasitol Res 2020; 119 (5):1629-1640 EhRab21 associates with the Golgi apparatus in Entamoeba histolytica.
Application : WB
Ye J et al. Cell Signal 2020; 69:109567 Nuclear import of NLS- RARα is mediated by importin α/β.
Application : WB
Cao C et al. Front Microbiol 2019; 10:3040 BICP0 Negatively Regulates TRAF6-Mediated NF-庥B and Interferon Activation by Promoting K48-Linked Polyubiquitination of TRAF6.
Application : WB
Chang CH et al. Dev Cell 2019; 48 (2):184-199.e5 Atoh1 Controls Primary Cilia Formation to Allow for SHH-Triggered Granule Neuron Progenitor Proliferation.
Chen WC et al. J Viral Hepat 2019; (Epub) MicroRNA-let-7c suppresses hepatitis C virus replication by targeting Bach1 for induction of haem oxygenase-1 expression.
Application : WB
Qin J et al. Avian Pathol 2018;:1月10日 Biological characteristics and immunological properties in Muscovy ducks of H5N6 virus-like particles composed of HA-TM/HA-TMH3 and M1.
Application : WB
Lin CK et al. J Infect Dis 2018; (Epub) Prostasin impairs epithelial growth factor receptor activation to suppress dengue virus propagation.
Application : WB
Yang D et al. Nat Commun 2018; 9 (1):3052 Sensing of cytosolic LPS through caspy2 pyrin domain mediates noncanonical inflammasome activation in zebrafish.
Application : WB
Lee HY et al. Oncotarget 2018; 9 (17):13193-13205 EIF3C-enhanced exosome secretion promotes angiogenesis and tumorigenesis of human hepatocellular carcinoma.
Application : WB
Liu GT et al. FASEB J 2018;:fj201701253R Improving nuclear envelope dynamics by EBV BFRF1 facilitates intranuclear component clearance through autophagy.
Application : ICC/IF
Omoto T et al. Oncogene 2017; (Epub) The impact of stromal Hic-5 on the tumorigenesis of colorectal cancer through lysyl oxidase induction and stromal remodeling.
Application : WB
Wang CY et al. Am J Cancer Res 2017; 7 (11):2103-2120 PSMB5 plays a dual role in cancer development and immunosuppression.
Application : WB
Kubo Y et al. Front Microbiol 2017; 8:1653 Rab3a-Bound CD63 Is Degraded and Rab3a-Free CD63 Is Incorporated into HIV-1 Particles.
Application : WB
Barajas D et al. PLoS One 2017; 12 (3):e0173010 Generation of infectious recombinant Adeno-associated virus in Saccharomyces cerevisiae.
Su KH et al. Nat Cell Biol 2016; 18 (5):527-39 HSF1 critically attunes proteotoxic stress sensing by mTORC1 to combat stress and promote growth.
Application : WB
Tien SC et al. Nat Commun 2016; 7:10420 The Shp2-induced epithelial disorganization defect is reversed by HDAC6 inhibition independent of Cdc42.
Application : WB
Braso-Maristany F et al. Nat Med 2016; 22 (11):1303-1313 PIM1 kinase regulates cell death, tumor growth and chemotherapy response in triple-negative breast cancer.
Cao XL et al. Protein Expr Purif 2016; 130:81-89 Expression and purification of mouse Ttyh1 fragments as antigens to generate Ttyh1-specific monoclonal antibodies.
Lee CP et al. J Virol 2016; The Ubiquitin Ligase Itch and Ubiquitination Regulate BFRF1-mediated Nuclear Envelope Modification for EBV Maturation.
Application : ICC/IF
Lin TJ et al. Sci Rep 2016; 6:27768 Inhibition of endosomal fusion activity of influenza virus by Rheum tanguticum (da-huang).
Application : WB
Skieterska K et al. Cell Signal 2016; 28 (8):1001-1014 Characterization of the interaction between the * D4 receptor, KLHL12 and β-arrestins.
Application : WB
Chang AT et al. Genes Dev 2015; 29 (6):603-16 An evolutionarily conserved DNA architecture determines target specificity of the TWIST family bHLH transcription factors.
Chen YA et al. Sci Rep 2015; 5 (Epub):10667 The Cullin 4A/B-DDB1-Cereblon E3 Ubiquitin Ligase Complex Mediates the Degradation of CLC-1 Chloride Channels.
Application : WB
Hyvarinen S et al. J Biol Chem 2014; 289 (7):4295-306 Recognition of Malondialdehyde-modified Proteins by the C Terminus of Complement Factor H Is Mediated via the Polyanion Binding Site and Impaired by Mutations Found in Atypical Hemolytic Uremic Syndrome.
Ho LJ et al. PLoS One 2013; 8 (12):e82033 Ginkgo biloba extract individually inhibits JNK activation and induces c-Jun degradation in human chondrocytes: potential therapeutics for osteoarthritis.
Application : WB
Tsao HW et al. Proc Natl Acad Sci U S A 2013; 110 (39):15776-81 Ets-1 facilitates nuclear entry of NFAT proteins and their recruitment to the IL-2 promoter.
Application : WB
Lee CP et al. PLoS Pathog 2012; 8 (9):e1002904 The ESCRT machinery is recruited by the viral BFRF1 protein to the nucleus-associated membrane for the maturation of Epstein-Barr Virus.
Application : ICC/IF
Lin CS et al. Cardiovasc Res 2012; 95 (3):346-55 PKCδ signalling regulates SR-A and CD36 expression and foam cell formation.
Wang X et al. Cancer Sci 2012; 103 (3):515-21 calcineurin subunit b upregulates β-* production by phosphorylation of * regulatory factor 3 via toll-like receptor 4.
Application : WB
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169