GeneTex
United States (US)

HA tag antibody

GTX115044_40219_IFA_18121410_553.jpg
GTX115044_41423_20150529_ELISA_18121410_123.jpg
GTX115044_41423_20150514_IP_18121410_624.jpg
GTX115044_41570_WB_B_18121410_563.jpg

Cat No. GTX115044

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP, ELISA
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IP 1:100-1:500
ELISA 1:1000-1:10000
Not tested in other applications.

Positive Control

HA-tagged protein expressing 293T
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Concentration

1mg/ml

Immunogen

The immunogen used to generate this antibody corresponds to HA tag

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HA tag antibody, HA antibody, HA epitope tag antibody
DATA IMAGES
GTX115044_40219_IFA_18121410_553.jpg

GTX115044 ICC/IF Image

Immunofluorescence analysis of HA-recombinant protein transfected HeLa cell which fixed with paraformaldehyde, using HA tag(GTX115044) antibody at 1:500 dilution.

GTX115044_41423_20150529_ELISA_18121410_123.jpg

GTX115044 ELISA Image

An ELISA plate is coated with 50 μL of a DDDDK Tag conjugated BSA at concentration of 5 μg/mL. The peptide conjugated carrier protein is coated and detected with anti-DDDDK Tag antibody (GTX115044) at concentration ranged from 3.1 to 200 ng/mL.

GTX115044_41423_20150514_IP_18121410_624.jpg

GTX115044 IP Image

Immunoprecipitation of HA tag protein from 293T whole cell extracts using 5 μg of HA tag antibody (GTX115044).
Western blot analysis was performed using HA tag antibody (GTX115044).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX115044_41570_WB_B_18121410_563.jpg

GTX115044 WB Image

HA Tag antibody detects HA Tag protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of HA-tagged protein expressing 293T cells
7.5 % SDS-PAGE
HA Tag antibody (GTX115044) dilution: 1:5000

Reference
REVIEW

HA tag antibody Cat No. GTX115044

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 27-Oct-2016
Score :
Application Tested : WB
Sample : HA-human ApoA1-transfected HeLa cells
Amount used : 15μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:20000, 4°C, overnightHr
Date : Anonymous submitted on 27-Oct-2016
Score :
Application Tested : ICC/IF
Sample : HA-human ApoA1-transfected HeLa cells
Blocking : 3% BSA, 25°C, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.05% saponin
Primary Antibody : 1:2000, 4°C, overnightHr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169