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HA tag antibody

Anti-HA tag antibody used in Immunoprecipitation (IP). GTX115044
Anti-HA tag antibody used in Western Blot (WB). GTX115044
Anti-HA tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115044
Anti-HA tag antibody used in ELISA (ELISA). GTX115044

Cat No. GTX115044

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP, ELISA
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IP 1:100-1:500
ELISA 1:1000-1:10000
Not tested in other applications.

Positive Control

HA-tagged protein expressing 293T
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to HA tag

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HA tag antibody , HA antibody , HA epitope tag antibody

Research Area

DATA IMAGES
Anti-HA tag antibody used in Immunoprecipitation (IP). GTX115044

GTX115044 IP Image

Immunoprecipitation of HA tag protein from 293T whole cell extracts using 5 µg of HA tag antibody (GTX115044).
Western blot analysis was performed using HA tag antibody (GTX115044).
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01) was used to detect the primary antibody.

Anti-HA tag antibody used in Western Blot (WB). GTX115044

GTX115044 WB Image

HA Tag antibody detects HA Tag protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of HA-tagged protein expressing 293T cells
7.5 % SDS-PAGE
HA Tag antibody (GTX115044) dilution: 1:5000

Anti-HA tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115044

GTX115044 ICC/IF Image

Immunofluorescence analysis of HA-recombinant protein transfected HeLa cell which fixed with paraformaldehyde, using HA tag(GTX115044) antibody at 1:500 dilution.

Anti-HA tag antibody used in ELISA (ELISA). GTX115044

GTX115044 ELISA Image

An ELISA plate is coated with 50 μL of a DDDDK Tag conjugated BSA at concentration of 5 μg/mL. The peptide conjugated carrier protein is coated and detected with anti-DDDDK Tag antibody (GTX115044) at concentration ranged from 3.1 to 200 ng/mL.

REFERENCE
REVIEW

HA tag antibody Cat No. GTX115044

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 27-Oct-2016
Score :
Application Tested : WB
Sample : HA-human ApoA1-transfected HeLa cells
Amount used : 15μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:20000, 4°C, 16Hr
Date : Anonymous submitted on 27-Oct-2016
Score :
Application Tested : ICC/IF
Sample : HA-human ApoA1-transfected HeLa cells
Blocking : 3% BSA, 25°C, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.05% saponin
Primary Antibody : 1:2000, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169