Suggested starting dilutions are as follows: WB: 1:1000-1:10000, ICC/IF: 1:100-1:1000, IP: 1:100-1:500, ELISA. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
ELISA: The anti HA diluted 1:70,000 gave an O.D.=1.0 in a 15 minute reaction against peptide conjugated with a different carrier than used for anti peptide purification. HRP conjugated Goat anti rabbit IgG was used and TMB was the substrate.
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.35 mg/ml (Please refer to the vial label for the specific concentration.)
YPYDVPDYA (influenza hemagglutinin-HA-epitope) conjugated to KLH
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
HA epitope tag , HA tag , HA
The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been extensively used as a general epitope tag in expression vectors.