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HA tag antibody

Anti-HA tag antibody used in Western Blot (WB). GTX29110
Anti-HA tag antibody used in Western Blot (WB). GTX29110
Anti-HA tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX29110
Anti-HA tag antibody used in Western Blot (WB). GTX29110
Anti-HA tag antibody used in Western Blot (WB). GTX29110
Anti-HA tag antibody used in Immunoprecipitation (IP). GTX29110
Anti-HA tag antibody used in Western Blot (WB). GTX29110

Cat No. GTX29110

Reference 16
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP, ELISA
Package
100 μl ($239)
See all HA tag products

APPLICATION

Application Note

Suggested starting dilutions are as follows: WB: 1:1000-1:10000, ICC/IF: 1:100-1:1000, IP: 1:100-1:500, ELISA. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Specificity/Sensitivity

ELISA: The anti HA diluted 1:70,000 gave an O.D.=1.0 in a 15 minute reaction against peptide conjugated with a different carrier than used for anti peptide purification. HRP conjugated Goat anti rabbit IgG was used and TMB was the substrate.

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.35 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

YPYDVPDYA (influenza hemagglutinin-HA-epitope) conjugated to KLH

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_371516

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

HA epitope tag , HA tag , HA

Cellular Localization

various

Background

The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been extensively used as a general epitope tag in expression vectors.

Research Area

DATA IMAGES

Anti-HA tag antibody used in Western Blot (WB). GTX29110

GTX29110 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HA tag antibody (GTX29110) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HA tag antibody used in Western Blot (WB). GTX29110

GTX29110 WB Image

Western Blot: HA Epitope Tag Antibody (GTX29110) - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).

Anti-HA tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX29110

GTX29110 ICC/IF Image

Immunocytochemistry/Immunofluorescence: HA Epitope Tag Antibody (GTX29110) - CHO cell

Anti-HA tag antibody used in Western Blot (WB). GTX29110

GTX29110 WB Image

Western blot detection of 1 ug of recombinant E. coli protein (containing 11 different epitopes) using GTX29110. Lane 1 contains the antibody (1:1000) alone, lane 2 contains the antibody and neutralizing peptide.

Anti-HA tag antibody used in Western Blot (WB). GTX29110

GTX29110 WB Image

Western Blot: HA Epitope Tag Antibody (GTX29110) - Detection of HA-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multitag fusion protein. Affinity purified rabbit anti-HA antibody GTX29110 used for WB at 0.04 ug/ml (1:25,000).

Anti-HA tag antibody used in Immunoprecipitation (IP). GTX29110

GTX29110 IP Image

Immunoprecipitation: HA Epitope Tag Antibody (GTX29110) - Immunoprecipitation: HA Epitope Tag Antibody- Samples: Lysate(150 mcg) from cells that were untransfected or stably transfected with a construct encoding an HA-tagged HIF 1-alpha truncation mutant. Affinity purified rabbit anti-HA used at 3mcg -150mcg lysate. Immunoprecipitated HA-HIF-1 alpha was detected using a monoclonal antibody to HIF-1 alpha.

Anti-HA tag antibody used in Western Blot (WB). GTX29110

GTX29110 WB Image

Western Blot: HA Epitope Tag Antibody (GTX29110) - Western Blot: HA Epitope Tag Antibody-Samples: Extracts from S. Cerevisae co-transformed with plasmids expressing HA Tag G alpha and HA tagged RGS domain of RGS beta were screened by Western Blot to identify clones expressing both proteins. Antibody: affinity purifed rabbit anti-HA used at 0.1 mcg/ml

REFERENCE

Application Reference

Crans RAJ et al. Front Pharmacol 2020; 11 : 194 Striatal Dopamine D 2-Muscarinic Acetylcholine M 1 Receptor-Receptor Interaction in a Model of Movement Disorders.
Application : WB
Gwon D et al. Mol Cells 2019; 42 (12) : 840-849 c-Cbl Acts as an E3 Ligase Against DDA3 for Spindle Dynamics and Centriole Duplication during Mitosis.
Application : ICC/IF
Vasudevan L et al. Biomolecules 2019; 9 (8) Heterodimerization of Mu Opioid Receptor Protomer with Dopamine D2 Receptor Modulates Agonist-Induced Internalization of Mu Opioid Receptor.
Application : WB
Herrmann AK et al. Hum Gene Ther 2018; (Epub) Impact of the assembly-activating protein (AAP) on molecular evolution of synthetic Adeno-associated virus (AAV) capsids.
Application : ICC/IF
Grosse S et al. J Virol 2017; 91 (20) Relevance of Assembly-Activating Protein for Adeno-associated Virus Vector Production and Capsid Protein Stability in Mammalian and Insect Cells.
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Submit a Reference

REVIEW

HA tag antibody Cat No. GTX29110

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Immunoprecipitation(IP)
( Average 5 based on 1 users reviews)
Anti-HA tag antibody used in Western Blot and Immunoprecipitation (WB IP). GTX29110
Date : Anonymous submitted on 23-Dec-2020
Score :
Application Tested : WB,IP
Figure Note : The data was pear reviewed and published in the journal Front Pharmacol in 2020.PMID: 32231561
Anti-HA tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX29110
Date : Anonymous submitted on 23-Dec-2020
Score :
Application Tested : ICC/IF
Figure Note : The data was pear reviewed and published in the journal BMC Cell Biol in 2009.PMID: 19686593
Submit a Review
Application Reference
Crans RAJ et al. Front Pharmacol 2020; 11:194 Striatal Dopamine D 2-Muscarinic Acetylcholine M 1 Receptor-Receptor Interaction in a Model of Movement Disorders.
Application : WB
Gwon D et al. Mol Cells 2019; 42 (12):840-849 c-Cbl Acts as an E3 Ligase Against DDA3 for Spindle Dynamics and Centriole Duplication during Mitosis.
Application : ICC/IF
Vasudevan L et al. Biomolecules 2019; 9 (8) Heterodimerization of Mu Opioid Receptor Protomer with Dopamine D2 Receptor Modulates Agonist-Induced Internalization of Mu Opioid Receptor.
Application : WB
Herrmann AK et al. Hum Gene Ther 2018; (Epub) Impact of the assembly-activating protein (AAP) on molecular evolution of synthetic Adeno-associated virus (AAV) capsids.
Application : ICC/IF
Grosse S et al. J Virol 2017; 91 (20) Relevance of Assembly-Activating Protein for Adeno-associated Virus Vector Production and Capsid Protein Stability in Mammalian and Insect Cells.
Leu JS et al. Am J Respir Crit Care Med 2016; SP110b Controls Host Immunity and Susceptibility to Tuberculosis.
Application : WB
Su CI et al. J Virol 2016; SUMO Modification Stabilizes Dengue Virus Nonstructural Protein 5.
Application : WB
Skieterska K et al. PLoS One 2015; 10 (12):e0145654 KLHL12 Promotes Non-Lysine Ubiquitination of the * Receptors D4.2 and D4.4, but Not of the ADHD-Associated D4.7 Variant.
Application : WB
Chang CW et al. J Virol 2015; 89 (3):1703-18 BGLF4 kinase modulates the structure and transport preference of the nuclear pore complex to facilitate nuclear import of Epstein-Barr virus lytic proteins.
Matthys A et al. Cell Signal 2012; 24 (5):1053-63 RhoBTB3 interacts with the 5-HT7a receptor and inhibits its proteasomal degradation.
Wang X et al. Cancer Sci 2012; 103 (3):515-21 calcineurin subunit b upregulates β-* production by phosphorylation of * regulatory factor 3 via toll-like receptor 4.
Van Craenenbroeck K et al. FEBS J 2011; 278 (8):1333-44 Dopamine D4 receptor oligomerization--contribution to receptor biogenesis.
Martin L et al. J Biol Chem 2010; 285 (42):31944-53 Regulation of the unfolded protein response by eif2Bdelta isoforms.
Application : IP
Anthonio EA et al. BMC Cell Biol 2009; 10:58 Small G proteins in peroxisome biogenesis: the potential involvement of ADP-ribosylation factor 6.
Application : ICC/IF
Huveneers S et al. Cancer Res 2007; 67 (6):2693-700 Integrin alpha v beta 3 controls activity and oncogenic potential of primed c-Src.
Ogden SK et al. J Biol Chem 2006; 281 (11):7237-43 Smoothened regulates activator and repressor functions of Hedgehog signaling via two distinct mechanisms.
Application : ICC/IF
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 239