GeneTex
United States (US)

HA tag antibody

HA-tag-antibody_WB_GTX29110-1_18121411_392.jpg
HA-tag-antibody_ICCIF_GTX29110-1_18121411_395.jpg
HA-tag-antibody-GTX29110-WB-1_18121411_875.jpg
HA-tag-antibody_WB_GTX29110-3_18121411_943.jpg
HA-tag-antibody_IP_GTX29110-1_18121411_975.jpg
HA-tag-antibody_WB_GTX29110-2_18121411_726.jpg

Cat No. GTX29110

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP, ELISA
APPLICATION

Application Note

Suggested starting dilutions are as follows: WB: 1:1000-1:10000, ICC/IF: 1:100-1:1000, IP: 1:100-1:500, ELISA. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Specificity/Sensitivity

ELISA: The anti HA diluted 1:70,000 gave an O.D.=1.0 in a 15 minute reaction against peptide conjugated with a different carrier than used for anti peptide purification. HRP conjugated Goat anti rabbit IgG was used and TMB was the substrate.
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Immunogen

YPYDVPDYA (influenza hemagglutinin-HA-epitope) conjugated to KLH

Purification

IgG fraction

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HA epitope tag, HA tag, HA

Cellular Localization

various

Background

The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been extensively used as a general epitope tag in expression vectors.

Research Area

DATA IMAGES
HA-tag-antibody_WB_GTX29110-1_18121411_392.jpg

GTX29110 WB Image

Western Blot: HA Epitope Tag Antibody (GTX29110) - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).

HA-tag-antibody_ICCIF_GTX29110-1_18121411_395.jpg

GTX29110 ICC/IF Image

Immunocytochemistry/Immunofluorescence: HA Epitope Tag Antibody (GTX29110) - CHO cell

HA-tag-antibody-GTX29110-WB-1_18121411_875.jpg

GTX29110 WB Image

Western blot detection of 1 ug of recombinant E. coli protein (containing 11 different epitopes) using GTX29110. Lane 1 contains the antibody (1:1000) alone, lane 2 contains the antibody and neutralizing peptide.

HA-tag-antibody_WB_GTX29110-3_18121411_943.jpg

GTX29110 WB Image

Western Blot: HA Epitope Tag Antibody (GTX29110) - Detection of HA-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multitag fusion protein. Affinity purified rabbit anti-HA antibody GTX29110 used for WB at 0.04 ug/ml (1:25,000).

HA-tag-antibody_IP_GTX29110-1_18121411_975.jpg

GTX29110 IP Image

Immunoprecipitation: HA Epitope Tag Antibody (GTX29110) - Immunoprecipitation: HA Epitope Tag Antibody- Samples: Lysate(150 mcg) from cells that were untransfected or stably transfected with a construct encoding an HA-tagged HIF 1-alpha truncation mutant. Affinity purified rabbit anti-HA used at 3mcg -150mcg lysate. Immunoprecipitated HA-HIF-1 alpha was detected using a monoclonal antibody to HIF-1 alpha.

HA-tag-antibody_WB_GTX29110-2_18121411_726.jpg

GTX29110 WB Image

Western Blot: HA Epitope Tag Antibody (GTX29110) - Western Blot: HA Epitope Tag Antibody-Samples: Extracts from S. Cerevisae co-transformed with plasmids expressing HA Tag G alpha and HA tagged RGS domain of RGS beta were screened by Western Blot to identify clones expressing both proteins. Antibody: affinity purifed rabbit anti-HA used at 0.1 mcg/ml

REFERENCE
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 239