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HA tag antibody

Anti-HA tag antibody used in Western Blot (WB). GTX29110
Anti-HA tag antibody used in Western Blot (WB). GTX29110
Anti-HA tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX29110
Anti-HA tag antibody used in Western Blot (WB). GTX29110
Anti-HA tag antibody used in Western Blot (WB). GTX29110
Anti-HA tag antibody used in Immunoprecipitation (IP). GTX29110
Anti-HA tag antibody used in Western Blot (WB). GTX29110

Cat No. GTX29110

Reference 14

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP, ELISA
Package
100 μl ($239)
See all HA tag products

APPLICATION

Application Note

Suggested starting dilutions are as follows: WB: 1:1000-1:10000, ICC/IF: 1:100-1:1000, IP: 1:100-1:500, ELISA. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Specificity/Sensitivity

ELISA: The anti HA diluted 1:70,000 gave an O.D.=1.0 in a 15 minute reaction against peptide conjugated with a different carrier than used for anti peptide purification. HRP conjugated Goat anti rabbit IgG was used and TMB was the substrate.

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.35 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

YPYDVPDYA (influenza hemagglutinin-HA-epitope) conjugated to KLH

Purification

IgG fraction

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

HA epitope tag, HA tag, HA

Cellular Localization

various

Background

The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been extensively used as a general epitope tag in expression vectors.

Research Area

DATA IMAGES

Anti-HA tag antibody used in Western Blot (WB). GTX29110

GTX29110 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HA tag antibody (GTX29110) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HA tag antibody used in Western Blot (WB). GTX29110

GTX29110 WB Image

Western Blot: HA Epitope Tag Antibody (GTX29110) - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).

Anti-HA tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX29110

GTX29110 ICC/IF Image

Immunocytochemistry/Immunofluorescence: HA Epitope Tag Antibody (GTX29110) - CHO cell

Anti-HA tag antibody used in Western Blot (WB). GTX29110

GTX29110 WB Image

Western blot detection of 1 ug of recombinant E. coli protein (containing 11 different epitopes) using GTX29110. Lane 1 contains the antibody (1:1000) alone, lane 2 contains the antibody and neutralizing peptide.

Anti-HA tag antibody used in Western Blot (WB). GTX29110

GTX29110 WB Image

Western Blot: HA Epitope Tag Antibody (GTX29110) - Detection of HA-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multitag fusion protein. Affinity purified rabbit anti-HA antibody GTX29110 used for WB at 0.04 ug/ml (1:25,000).

Anti-HA tag antibody used in Immunoprecipitation (IP). GTX29110

GTX29110 IP Image

Immunoprecipitation: HA Epitope Tag Antibody (GTX29110) - Immunoprecipitation: HA Epitope Tag Antibody- Samples: Lysate(150 mcg) from cells that were untransfected or stably transfected with a construct encoding an HA-tagged HIF 1-alpha truncation mutant. Affinity purified rabbit anti-HA used at 3mcg -150mcg lysate. Immunoprecipitated HA-HIF-1 alpha was detected using a monoclonal antibody to HIF-1 alpha.

Anti-HA tag antibody used in Western Blot (WB). GTX29110

GTX29110 WB Image

Western Blot: HA Epitope Tag Antibody (GTX29110) - Western Blot: HA Epitope Tag Antibody-Samples: Extracts from S. Cerevisae co-transformed with plasmids expressing HA Tag G alpha and HA tagged RGS domain of RGS beta were screened by Western Blot to identify clones expressing both proteins. Antibody: affinity purifed rabbit anti-HA used at 0.1 mcg/ml

REFERENCE

Application Reference

Gwon D et al. Mol Cells 2019; 42 (12) : 840-849 c-Cbl Acts as an E3 Ligase Against DDA3 for Spindle Dynamics and Centriole Duplication during Mitosis.
Application : ICC/IF
Vasudevan L et al. Biomolecules 2019; 9 (8) Heterodimerization of Mu Opioid Receptor Protomer with Dopamine D2 Receptor Modulates Agonist-Induced Internalization of Mu Opioid Receptor.
Application : WB
Herrmann AK et al. Hum Gene Ther 2018; (Epub) Impact of the assembly-activating protein (AAP) on molecular evolution of synthetic Adeno-associated virus (AAV) capsids.
Application : ICC/IF
Leu JS et al. Am J Respir Crit Care Med 2016; SP110b Controls Host Immunity and Susceptibility to Tuberculosis.
Application : WB
Su CI et al. J Virol 2016; SUMO Modification Stabilizes Dengue Virus Nonstructural Protein 5.
Application : WB
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REVIEW

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Application Reference
Gwon D et al. Mol Cells 2019; 42 (12):840-849 c-Cbl Acts as an E3 Ligase Against DDA3 for Spindle Dynamics and Centriole Duplication during Mitosis.
Application : ICC/IF
Vasudevan L et al. Biomolecules 2019; 9 (8) Heterodimerization of Mu Opioid Receptor Protomer with Dopamine D2 Receptor Modulates Agonist-Induced Internalization of Mu Opioid Receptor.
Application : WB
Herrmann AK et al. Hum Gene Ther 2018; (Epub) Impact of the assembly-activating protein (AAP) on molecular evolution of synthetic Adeno-associated virus (AAV) capsids.
Application : ICC/IF
Leu JS et al. Am J Respir Crit Care Med 2016; SP110b Controls Host Immunity and Susceptibility to Tuberculosis.
Application : WB
Su CI et al. J Virol 2016; SUMO Modification Stabilizes Dengue Virus Nonstructural Protein 5.
Application : WB
Skieterska K et al. PLoS One 2015; 10 (12):e0145654 KLHL12 Promotes Non-Lysine Ubiquitination of the * Receptors D4.2 and D4.4, but Not of the ADHD-Associated D4.7 Variant.
Application : WB
Chang CW et al. J Virol 2015; 89 (3):1703-18 BGLF4 kinase modulates the structure and transport preference of the nuclear pore complex to facilitate nuclear import of Epstein-Barr virus lytic proteins.
Matthys A et al. Cell Signal 2012; 24 (5):1053-63 RhoBTB3 interacts with the 5-HT7a receptor and inhibits its proteasomal degradation.
Wang X et al. Cancer Sci 2012; 103 (3):515-21 calcineurin subunit b upregulates β-* production by phosphorylation of * regulatory factor 3 via toll-like receptor 4.
Van Craenenbroeck K et al. FEBS J 2011; 278 (8):1333-44 Dopamine D4 receptor oligomerization--contribution to receptor biogenesis.
Martin L et al. J Biol Chem 2010; 285 (42):31944-53 Regulation of the unfolded protein response by eif2Bdelta isoforms.
Application : IP
Anthonio EA et al. BMC Cell Biol 2009; 10:58 Small G proteins in peroxisome biogenesis: the potential involvement of ADP-ribosylation factor 6.
Application : ICC/IF
Huveneers S et al. Cancer Res 2007; 67 (6):2693-700 Integrin alpha v beta 3 controls activity and oncogenic potential of primed c-Src.
Ogden SK et al. J Biol Chem 2006; 281 (11):7237-43 Smoothened regulates activator and repressor functions of Hedgehog signaling via two distinct mechanisms.
Application : ICC/IF
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 239