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HA tag antibody

Cat No. GTX80896

Host Rabbit
Clonality Polyclonal
Application WB
Package
500μl ($289)
APPLICATION

Application Note

For WB: Use at a dilution of 1:200. This antibody detects the presence of HA-tagged proteins. Optimal dilutions/concentrations should be determined by the end user.

Specificity/Sensitivity

Detects HA tagged proteins
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.2, containing 0.1 % sodium azide and 0.2% gelatin

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Synthetic Peptide: YPYDVPDYA

Purification

Affinity purified with antigen
From polyclonal serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HA epitope tag

Background

Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the peptide epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the peptide tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to proteins from each newly cloned gene. Commonly used epitope tags include glutathione-S-transferase (GST), c-myc, 6-histidine (6X-His), FLAG, green fluorescent protein (GFP), maltose binding protein (MBP), influenza A virus haemagglutinin (HA), b-galactosidase, and GAL4.

Research Area

REFERENCE
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Package List Price ($)
$ 289