GeneTex
United States (US)

HACL1 antibody

GTX106858_39959_IHC_19011506_849.jpg
GTX106858_WB_19011506_442.jpg
GTX106858_IFA_19011506_547.jpg
GTX106858_39959_IHC_2_19011506_782.jpg
GTX106858_39959_WB_M_liver_19011506_982.jpg

Cat No. GTX106858

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

293T , mouse liver

Predict Reactivity

Rat, Bovine, Pig, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human HACL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

2-HPCL antibody, FLJ55041 antibody, HPCL antibody, HPCL2 antibody, PHYH2 antibody, HACL1 antibody, 2-hydroxyphytanol-CoA lyase antibody, phytanoyl-CoA 2-hydroxylase 2 antibody, 2-hydroxyacyl-CoA lyase 1 antibody, 1600020H07Rik antibody, 2-hydroxyphytanoyl-CoA lyase antibody, phytanoyl-CoA hydroxylase 2 antibody

Cellular Localization

Peroxisome

Background

Catalyzes a carbon-carbon cleavage reaction; cleaves a 2-hydroxy-3-methylacyl-CoA into formyl-CoA and a 2-methyl-branched fatty aldehyde.

Database

DATA IMAGES
GTX106858_39959_IHC_19011506_849.jpg

GTX106858 IHC-P Image

HACL1 antibody detects HACL1 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
HACL1 antibody (GTX106858) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106858_WB_19011506_442.jpg

GTX106858 WB Image

Sample(30 ug whole cell lysate)
A:293T
7.5% SDS PAGE
GTX106858 diluted at 1:1000

GTX106858_IFA_19011506_547.jpg

GTX106858 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HACL1(GTX106858) antibody at 1:200 dilution.

GTX106858_39959_IHC_2_19011506_782.jpg

GTX106858 IHC-P Image

HACL1 antibody detects HACL1 protein at cytosol on human normal liver by immunohistochemical analysis.
Sample: Paraffin-embedded human normal liver.
HACL1 antibody (GTX106858) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106858_39959_WB_M_liver_19011506_982.jpg

GTX106858 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX106858 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169