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HADH antibody, Internal

Anti-HADH antibody, Internal used in Western Blot (WB). GTX88923
Anti-HADH antibody, Internal used in Western Blot (WB). GTX88923

Cat. No. GTX88923

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μg ($399)
See all HADH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.01-0.03μg/ml
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88923-PEP).

Predict Reactivity

Mouse, Rat, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-YERGDASKEDID, from the internal region of the protein sequence according to NP_005318.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hydroxyacyl-CoA dehydrogenase , HAD , HADH1 , HADHSC , HCDH , HHF4 , MSCHAD , SCHAD

Cellular Localization

Mitochondrion matrix

Background

This gene is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene of this gene on chromosome 15. [provided by RefSeq, May 2010]

Database

Research Area

DATA IMAGES

Anti-HADH antibody, Internal used in Western Blot (WB). GTX88923

GTX88923 WB Image

WB analysis of human kidney lysate using GTX88923 HADH antibody, Internal.
Dilution : 0.02μg/ml
Loading : 35μg protein in RIPA buffer

Anti-HADH antibody, Internal used in Western Blot (WB). GTX88923

GTX88923 WB Image

WB analysis of HEK293 overexpressing HADH with C-terminal tag (DYKDDDDK) using GTX88923 HADH antibody, Internal.
Left panel : tag antibody (mock transfection in first lanes)
Right panel : primary antibody (mock transfection in last lanes)

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399