APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 0.01-0.03μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88923-PEP).
Predict Reactivity
Mouse, Rat, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-YERGDASKEDID, from the internal region of the protein sequence according to NP_005318.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
hydroxyacyl-CoA dehydrogenase , HAD , HADH1 , HADHSC , HCDH , HHF4 , MSCHAD , SCHAD
Cellular Localization
Mitochondrion matrix
Background
This gene is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene of this gene on chromosome 15. [provided by RefSeq, May 2010]
Database
Research Area
DATA IMAGES
|
GTX88923 WB Image
WB analysis of HEK293 overexpressing HADH with C-terminal tag (DYKDDDDK) using GTX88923 HADH antibody, Internal.
Left panel : tag antibody (mock transfection in first lanes)
Right panel : primary antibody (mock transfection in last lanes)
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|
GTX88923 WB Image
WB analysis of human kidney lysate using GTX88923 HADH antibody, Internal.
Dilution : 0.02μg/ml
Loading : 35μg protein in RIPA buffer
|
REFERENCE
There are currently no references for HADH antibody, Internal (GTX88923). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for HADH antibody, Internal (GTX88923). Be the first to share your experience with this product.
![Anti-Citrate synthase antibody [GT1761] used in Western Blot (WB). GTX628143](/upload/website/prouct_img/small/GTX628143/GTX628143_41113_20191129_WB_Fraction_19120122_515.jpg "Anti-Citrate synthase antibody [GT1761] used in Western Blot (WB). GTX628143")
