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HADH antibody

Anti-HADH antibody used in Western Blot (WB). GTX105167
Anti-HADH antibody used in Western Blot (WB). GTX105167
Anti-HADH antibody used in IHC (Paraffin sections) (IHC-P). GTX105167
Anti-HADH antibody used in Western Blot (WB). GTX105167
Anti-HADH antibody used in Western Blot (WB). GTX105167
Anti-HADH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105167
Anti-HADH antibody used in Immunoprecipitation (IP). GTX105167
Anti-HADH antibody used in IHC (Paraffin sections) (IHC-P). GTX105167

Cat No. GTX105167

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , GL261 , C8D30 , PC-12

Predict Reactivity

Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HADH. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

hydroxyacyl-CoA dehydrogenase , HAD , HADH1 , HADHSC , HCDH , HHF4 , MSCHAD , SCHAD

Cellular Localization

Mitochondrion matrix

Background

This gene is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-HADH antibody used in Western Blot (WB). GTX105167

GTX105167 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with HADH antibody (GTX105167) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-HADH antibody used in Western Blot (WB). GTX105167

GTX105167 WB Image

HADH antibody detects HADH protein by Western blot analysis.
A. 30 µg GL261 whole cell lysate/extract
B. 30 ug C8D30 whole cell lysate/extract
10 % SDS-PAGE
HADH antibody (GTX105167) dilution: 1:1000

Anti-HADH antibody used in IHC (Paraffin sections) (IHC-P). GTX105167

GTX105167 IHC-P Image

HADH antibody detects HADH protein at mitochondria on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
HADH antibody (GTX105167) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HADH antibody used in Western Blot (WB). GTX105167

GTX105167 WB Image

HADH antibody detects HADH protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
10 % SDS-PAGE
HADH antibody (GTX105167) dilution: 1:1000

Anti-HADH antibody used in Western Blot (WB). GTX105167

GTX105167 WB Image

HADH antibody detects HADH protein by Western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg A431 whole cell lysate/extract
C. 30 ug HeLa whole cell lysate/extract
D. 30 ug HepG2 whole cell lysate/extract
10 % SDS-PAGE
HADH antibody (GTX105167) dilution: 1:1000

Anti-HADH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105167

GTX105167 ICC/IF Image

HADH antibody detects HADH protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: HADH protein stained by HADH antibody (GTX105167) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-HADH antibody used in Immunoprecipitation (IP). GTX105167

GTX105167 IP Image

Immunoprecipitation of HADH protein from HepG2 whole cell extracts using 5 μg of HADH antibody (GTX105167).
Western blot analysis was performed using HADH antibody (GTX105167).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-HADH antibody used in IHC (Paraffin sections) (IHC-P). GTX105167

GTX105167 IHC-P Image

HADH antibody detects HADH protein at mitochondria on mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
HADH antibody (GTX105167) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169