GeneTex
United States (US)

HADH antibody

KO/KD
GTX105167_40723_20181123_WB_shRNA_watermark_19011519_698.jpg
GTX105167_40723_WB_M_19011506_650.jpg
GTX105167_40723_20141024_IHC_M_2_19011506_216.jpg
GTX105167_40723_WB_R_19011506_339.jpg
GTX105167_40723_WB_19011506_368.jpg
GTX105167_40723_20170719_IFA_19011506_727.jpg
GTX105167_40723_20150522_IP_19011506_482.jpg
GTX105167_40723_20141024_IHC_M_19011506_595.jpg

Cat No. GTX105167

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , GL261 , C8D30 , PC-12

Predict Reactivity

Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HADH. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HAD antibody, HADH1 antibody, HADHSC antibody, HCDH antibody, HHF4 antibody, MGC8392 antibody, MSCHAD antibody, SCHAD antibody, HADH antibody, L-3-hydroxyacyl-Coenzyme A dehydrogenase, short chain antibody, hydroxyacyl-coenzyme A dehydrogenase, mitochondrial antibody, short-chain 3-hydroxyacyl-CoA dehydrogenase antibody, medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase antibody, hydroxyacyl-CoA dehydrogenase antibody

Cellular Localization

Mitochondrion matrix

Background

This gene is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX105167_40723_20181123_WB_shRNA_watermark_19011519_698.jpg

GTX105167 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with HADH antibody (GTX105167) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX105167_40723_WB_M_19011506_650.jpg

GTX105167 WB Image

HADH antibody detects HADH protein by Western blot analysis.
A. 30 µg GL261 whole cell lysate/extract
B. 30 ug C8D30 whole cell lysate/extract
10 % SDS-PAGE
HADH antibody (GTX105167) dilution: 1:1000

GTX105167_40723_20141024_IHC_M_2_19011506_216.jpg

GTX105167 IHC-P Image

HADH antibody detects HADH protein at mitochondria on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
HADH antibody (GTX105167) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105167_40723_WB_R_19011506_339.jpg

GTX105167 WB Image

HADH antibody detects HADH protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
10 % SDS-PAGE
HADH antibody (GTX105167) dilution: 1:1000

GTX105167_40723_WB_19011506_368.jpg

GTX105167 WB Image

HADH antibody detects HADH protein by Western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg A431 whole cell lysate/extract
C. 30 ug HeLa whole cell lysate/extract
D. 30 ug HepG2 whole cell lysate/extract
10 % SDS-PAGE
HADH antibody (GTX105167) dilution: 1:1000

GTX105167_40723_20170719_IFA_19011506_727.jpg

GTX105167 ICC/IF Image

HADH antibody detects HADH protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: HADH protein stained by HADH antibody (GTX105167) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX105167_40723_20150522_IP_19011506_482.jpg

GTX105167 IP Image

Immunoprecipitation of HADH protein from HepG2 whole cell extracts using 5 μg of HADH antibody (GTX105167).
Western blot analysis was performed using HADH antibody (GTX105167).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX105167_40723_20141024_IHC_M_19011506_595.jpg

GTX105167 IHC-P Image

HADH antibody detects HADH protein at mitochondria on mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
HADH antibody (GTX105167) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169