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HADH antibody

Anti-HADH antibody used in Western Blot (WB). GTX118325
Anti-HADH antibody used in IHC (Paraffin sections) (IHC-P). GTX118325
Anti-HADH antibody used in Western Blot (WB). GTX118325
Anti-HADH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118325
Anti-HADH antibody used in Western Blot (WB). GTX118325
Anti-HADH antibody used in Western Blot (WB). GTX118325

Cat No. GTX118325

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , mouse liver , rat heart , rat liver

Predict Reactivity

Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.49 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HADH. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617989

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

hydroxyacyl-CoA dehydrogenase , HAD , HADH1 , HADHSC , HCDH , HHF4 , MSCHAD , SCHAD

Cellular Localization

Mitochondrion matrix

Background

This gene is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HADH antibody used in Western Blot (WB). GTX118325

GTX118325 WB Image

Sample (50 μg of whole cell lysate)
A: mouse liver
10% SDS PAGE
GTX118325 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HADH antibody used in IHC (Paraffin sections) (IHC-P). GTX118325

GTX118325 IHC-P Image

HADH antibody detects HADH protein at mitochondria on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
HADH antibody (GTX118325) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HADH antibody used in Western Blot (WB). GTX118325

GTX118325 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HADH antibody (GTX118325) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HADH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118325

GTX118325 ICC/IF Image

HADH antibody detects HADH protein at Mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: HADH protein stained by HADH antibody (GTX118325) diluted at 1:500.
Blue: Hoechst 33343 staining.

Anti-HADH antibody used in Western Blot (WB). GTX118325

GTX118325 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HADH antibody (GTX118325) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HADH antibody used in Western Blot (WB). GTX118325

GTX118325 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HADH antibody (GTX118325) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf