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HADH antibody

Anti-HADH antibody used in Western Blot (WB). GTX118325
Anti-HADH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118325
Anti-HADH antibody used in Western Blot (WB). GTX118325
Anti-HADH antibody used in IHC (Paraffin sections) (IHC-P). GTX118325
Anti-HADH antibody used in Western Blot (WB). GTX118325
Anti-HADH antibody used in Western Blot (WB). GTX118325
Anti-HADH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118325

Cat. No. GTX118325

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all HADH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

Mouse liver , rat liver , rat heart

Predict Reactivity

Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.49 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HADH. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617989

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hydroxyacyl-CoA dehydrogenase , HAD , HADH1 , HADHSC , HCDH , HHF4 , MSCHAD , SCHAD

Cellular Localization

Mitochondrion matrix

Background

This gene is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HADH antibody used in Western Blot (WB). GTX118325

GTX118325 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HADH antibody (GTX118325) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HADH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118325

GTX118325 ICC/IF Image

HADH antibody detects HADH protein at mitochondria by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HADH stained by HADH antibody (GTX118325) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-HADH antibody used in Western Blot (WB). GTX118325

GTX118325 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HADH antibody (GTX118325) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HADH antibody used in IHC (Paraffin sections) (IHC-P). GTX118325

GTX118325 IHC-P Image

HADH antibody detects HADH protein at mitochondria on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
HADH antibody (GTX118325) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HADH antibody used in Western Blot (WB). GTX118325

GTX118325 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HADH antibody (GTX118325) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HADH antibody used in Western Blot (WB). GTX118325

GTX118325 WB Image

Sample (50 μg of whole cell lysate)
A: mouse liver
10% SDS PAGE
GTX118325 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HADH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118325

GTX118325 ICC/IF Image

HADH antibody detects HADH protein at Mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: HADH protein stained by HADH antibody (GTX118325) diluted at 1:500.
Blue: Hoechst 33343 staining.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169