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HADHA antibody [N2C1], Internal

KO/KD
Anti-HADHA antibody [N2C1], Internal used in Western Blot (WB). GTX113727
Anti-HADHA antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX113727
Anti-HADHA antibody [N2C1], Internal used in Western Blot (WB). GTX113727
Anti-HADHA antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113727
Anti-HADHA antibody [N2C1], Internal used in Western Blot (WB). GTX113727
Anti-HADHA antibody [N2C1], Internal used in Western Blot (WB). GTX113727
Anti-HADHA antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113727
Anti-HADHA antibody [N2C1], Internal used in Western Blot (WB). GTX113727

Cat. No. GTX113727

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all HADHA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

Mouse liver , PC-12 , 293T , A431 , 293T

Predict Reactivity

Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.37 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HADHA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037134

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha , ECHA , GBP , HADH , LCEH , LCHAD , MTPA , TP-ALPHA

Cellular Localization

Mitochondrion

Background

This gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HADHA antibody [N2C1], Internal used in Western Blot (WB). GTX113727

GTX113727 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HADHA antibody [N2C1], Internal (GTX113727) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HADHA antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX113727

GTX113727 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using HADHA(GTX113727) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HADHA antibody [N2C1], Internal used in Western Blot (WB). GTX113727

GTX113727 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX113727 diluted at 1:1000

Anti-HADHA antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113727

GTX113727 ICC/IF Image

HADHA antibody [N2C1], Internal detects HADHA protein at Mitochondia by immunofluorescent analysis.
Sample: HepG2 cells were fixed in -20ºC 100% MeOH for 5 min.
Green: HADHA protein stained by HADHA antibody [N2C1], Internal (GTX113727) diluted at 1:500.
Blue: Hoechst 33343 staining.
Anti-HADHA antibody [N2C1], Internal used in Western Blot (WB). GTX113727

GTX113727 WB Image

HADHA antibody [N2C1], Internal detects HADHA protein by Western blot analysis.
A.30 μg PC-12 whole cell lysate/extract
7.5 % SDS-PAGE
HADHA antibody [N2C1], Internal (GTX113727) dilution: 1:500

Anti-HADHA antibody [N2C1], Internal used in Western Blot (WB). GTX113727

GTX113727 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
7.5% SDS PAGE
GTX113727 diluted at 1:1000

Anti-HADHA antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113727

GTX113727 ICC/IF Image

HADHA antibody [N2C1], Internal detects HADHA protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HADHA protein stained by HADHA antibody [N2C1], Internal (GTX113727) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-HADHA antibody [N2C1], Internal used in Western Blot (WB). GTX113727

GTX113727 WB Image

The data was published in the journal BMC Biochem in 2014. PMID: 25152047

REFERENCE

Application Reference

Dang Y et al. Biomed Pharmacother 2020; 127 : 110127 Gan-Jiang-Ling-Zhu decoction alleviates hepatic steatosis in rats by the miR-138-5p/CPT1B axis.
Application : WB
Reactivity : Rat
Harel M et al. Cell 2019; (Epub) Proteomics of Melanoma Response to Immunotherapy Reveals Mitochondrial Dependence.
Application : IHC-P
Reactivity : Human
Lee J et al. Cell Rep 2016; 16 (1) : 201-12 Hepatic Fatty Acid Oxidation Restrains Systemic Catabolism during Starvation.
Application : WB
Reactivity : Mouse
Lee J et al. Cell Rep 2015; 10 (2) : 266-279 Adipose Fatty Acid Oxidation Is Required for Thermogenesis and Potentiates Oxidative Stress-Induced Inflammation.
Application : WB
Reactivity : Mouse
Rodriguez S et al. BMC Biochem 2014; 15 (1) : 20 Chemical-genetic induction of Malonyl-CoA decarboxylase in skeletal muscle.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169