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HADHA antibody

KO/KD
Anti-HADHA antibody used in Western Blot (WB). GTX101177
Anti-HADHA antibody used in Western Blot (WB). GTX101177
Anti-HADHA antibody used in Western Blot (WB). GTX101177
Anti-HADHA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101177
Anti-HADHA antibody used in Western Blot (WB). GTX101177
Anti-HADHA antibody used in IHC (Paraffin sections) (IHC-P). GTX101177

Cat. No. GTX101177

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all HADHA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

mouse kidney , rat liver , Molt-4 , Raji , 293T

Predict Reactivity

Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human HADHA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950479

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha , ECHA , GBP , HADH , LCEH , LCHAD , MTPA , TP-ALPHA

Cellular Localization

Mitochondrion

Background

This gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HADHA antibody used in Western Blot (WB). GTX101177

GTX101177 WB Image

HADHA antibody detects HADHA protein by Western blot analysis.
A.50 μg rat liver lysate/extract
7.5 % SDS-PAGE
HADHA antibody (GTX101177) dilution: 1:500

Anti-HADHA antibody used in Western Blot (WB). GTX101177

GTX101177 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HADHA antibody (GTX101177) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HADHA antibody used in Western Blot (WB). GTX101177

GTX101177 WB Image

HADHA antibody detects HADHA protein by Western blot analysis.
A. 50 μg mouse kidney lysate/extract
7.5 % SDS-PAGE
HADHA antibody (GTX101177) dilution: 1:500

Anti-HADHA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101177

GTX101177 ICC/IF Image

HADHA antibody detects HADHA protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: HADHA protein stained by HADHA antibody (GTX101177) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-HADHA antibody used in Western Blot (WB). GTX101177

GTX101177 WB Image

Sample(30 μg of whole cell lysate)
A:Molt-4 (GTX27912)
B:Raji(GTX27908)
7.5% SDS PAGE
GTX101177 diluted at 1:1500

Anti-HADHA antibody used in IHC (Paraffin sections) (IHC-P). GTX101177

GTX101177 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human stomach , using HADHA(GTX101177) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Selen ES et al. J Biol Chem 2022; 298 (12) : 102648 Requirement of hepatic pyruvate carboxylase during fasting, high fat, and ketogenic diet.
Application : WB
Reactivity : Mouse
Gonzalez-Hurtado E et al. Am J Physiol Endocrinol Metab 2017; 312 (5) : E381-E393 Loss of macrophage fatty acid oxidation does not potentiate systemic metabolic dysfunction.
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169