GeneTex
United States (US)

HADHA antibody

KO/KD
GTX101177_39904_20181026_WB_shRNA_watermark_19011519_549.jpg
GTX101177_WB_19011506_137.jpg
GTX101177_39904_WB_M_kidney_19011506_218.jpg
GTX101177_39904_20170719_IFA_19011506_447.jpg
GTX101177_39904_IHC_19011506_292.jpg
GTX101177_39904_WB_R_Liver_19011506_111.jpg

Cat No. GTX101177

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

Molt-4 , Raji , mouse kidney , rat liver

Predict Reactivity

Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human HADHA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Hydroxyacyl-Coa Dehydrogenase Trifunctional Multienzyme Complex Subunit Alpha,Echa,Gbp,Hadh,Lceh,Lchad,Mtpa,Tp-Alpha,Hadha

Cellular Localization

Mitochondrion

Background

This gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101177_39904_20181026_WB_shRNA_watermark_19011519_549.jpg

GTX101177 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HADHA antibody (GTX101177) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101177_WB_19011506_137.jpg

GTX101177 WB Image

Sample(30 μg of whole cell lysate)
A:Molt-4 (GTX27912)
B:Raji(GTX27908)
7.5% SDS PAGE
GTX101177 diluted at 1:1500

GTX101177_39904_WB_M_kidney_19011506_218.jpg

GTX101177 WB Image

HADHA antibody detects HADHA protein by Western blot analysis.
A. 50 µg mouse kidney lysate/extract
7.5 % SDS-PAGE
HADHA antibody (GTX101177) dilution: 1:500

GTX101177_39904_20170719_IFA_19011506_447.jpg

GTX101177 ICC/IF Image

HADHA antibody detects HADHA protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: HADHA protein stained by HADHA antibody (GTX101177) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX101177_39904_IHC_19011506_292.jpg

GTX101177 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human stomach , using HADHA(GTX101177) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101177_39904_WB_R_Liver_19011506_111.jpg

GTX101177 WB Image

HADHA antibody detects HADHA protein by Western blot analysis.
A.50 µg rat liver lysate/extract
7.5 % SDS-PAGE
HADHA antibody (GTX101177) dilution: 1:500

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169