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HADHA antibody

KO/KD
Anti-HADHA antibody used in Western Blot (WB). GTX32642
Anti-HADHA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32642
Anti-HADHA antibody used in Immunoprecipitation (IP). GTX32642
Anti-HADHA antibody used in Western Blot (WB). GTX32642

Cat. No. GTX32642

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse
Package
100 μl ($419)
See all HADHA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IP 1:50 - 1:100
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 484-763 of human HADHA (NP_000173.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha , ECHA , GBP , HADH , LCEH , LCHAD , MTPA , TP-ALPHA

Cellular Localization

Mitochondrion

Background

This gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-HADHA antibody used in Western Blot (WB). GTX32642

GTX32642 WB Image

WB analysis of various sample lysates using GTX32642 HADHA antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-HADHA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32642

GTX32642 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX32642 HADHA antibody.
Blue : DAPI

Anti-HADHA antibody used in Immunoprecipitation (IP). GTX32642

GTX32642 IP Image

IP analysis of HeLa cell lysate using GTX32642 HADHA antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

Anti-HADHA antibody used in Western Blot (WB). GTX32642

GTX32642 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) 293T cell lysate using GTX32642 HADHA antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 419