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HADHA antibody

Anti-HADHA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32642
Anti-HADHA antibody used in Western Blot (WB). GTX32642
Anti-HADHA antibody used in Western Blot (WB). GTX32642

Cat No. GTX32642

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 484-763 of human HADHA (NP_000173.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha , ECHA , GBP , HADH , LCEH , LCHAD , MTPA , TP-ALPHA

Cellular Localization

Mitochondrion

Background

This gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-HADHA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32642

GTX32642 ICC/IF Image

ICC/IF analysis of U2OS cell using GTX32642 HADHA antibody.
Blue : DAPI

Anti-HADHA antibody used in Western Blot (WB). GTX32642

GTX32642 WB Image

WB analysis of wild type and HADHA knockout cell lysate using GTX32642 HADHA antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-HADHA antibody used in Western Blot (WB). GTX32642

GTX32642 WB Image

WB analysis of various samples using GTX32642 HADHA antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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Package List Price ($)
$ 329