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HAGH antibody [N2C3]

Anti-HAGH antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX105708
Anti-HAGH antibody [N2C3] used in Western Blot (WB). GTX105708
Anti-HAGH antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX105708
Anti-HAGH antibody [N2C3] used in Western Blot (WB). GTX105708
Anti-HAGH antibody [N2C3] used in Western Blot (WB). GTX105708
Anti-HAGH antibody [N2C3] used in Western Blot (WB). GTX105708

Cat No. GTX105708

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($329),
25 μl ($169)
See all HAGH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

HepG2 , mouse liver

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HAGH. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

hydroxyacylglutathione hydrolase , GLO2 , GLX2 , GLXII , HAGH1

Background

The enzyme encoded by this gene is classified as a thiolesterase and is responsible for the hydrolysis of S-lactoyl-glutathione to reduced glutathione and D-lactate. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HAGH antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX105708

GTX105708 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using HAGH(GTX105708) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HAGH antibody [N2C3] used in Western Blot (WB). GTX105708

GTX105708 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
12% SDS PAGE
GTX105708 diluted at 1:1000

Anti-HAGH antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX105708

GTX105708 IHC-P Image

HAGH antibody [N2C3] detects HAGH protein at mitochondria on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
HAGH antibody [N2C3] (GTX105708) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HAGH antibody [N2C3] used in Western Blot (WB). GTX105708

GTX105708 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
10% SDS PAGE
GTX105708 diluted at 1:1000

Anti-HAGH antibody [N2C3] used in Western Blot (WB). GTX105708

GTX105708 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HAGH antibody [N2C3] (GTX105708) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HAGH antibody [N2C3] used in Western Blot (WB). GTX105708

GTX105708 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HAGH antibody [N2C3] (GTX105708) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169