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HAGH antibody [N2C3]

Anti-HAGH antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX105708
Anti-HAGH antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX105708
Anti-HAGH antibody [N2C3] used in Western Blot (WB). GTX105708
Anti-HAGH antibody [N2C3] used in Western Blot (WB). GTX105708

Cat No. GTX105708

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($329),
25 μl ($169)
See all HAGH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

HepG2 , mouse liver

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HAGH. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037135

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

hydroxyacylglutathione hydrolase , GLO2 , GLX2 , GLXII , HAGH1

Background

The enzyme encoded by this gene is classified as a thiolesterase and is responsible for the hydrolysis of S-lactoyl-glutathione to reduced glutathione and D-lactate. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HAGH antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX105708

GTX105708 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using HAGH(GTX105708) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HAGH antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX105708

GTX105708 IHC-P Image

HAGH antibody [N2C3] detects HAGH protein at mitochondria on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
HAGH antibody [N2C3] (GTX105708) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HAGH antibody [N2C3] used in Western Blot (WB). GTX105708

GTX105708 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HAGH antibody [N2C3] (GTX105708) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HAGH antibody [N2C3] used in Western Blot (WB). GTX105708

GTX105708 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HAGH antibody [N2C3] (GTX105708) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169