Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HAGH antibody [N2C3] (GTX105708) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HAGH antibody [N2C3] (GTX105708) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

HAGH antibody [N2C3] detects HAGH protein at mitochondria on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
HAGH antibody [N2C3] (GTX105708) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using HAGH(GTX105708) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min