Wild-type (WT) and HTT knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HAP40 antibody [HL2641] (GTX639097) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HAP40 antibody [HL2641] (GTX639097) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

HAP40 antibody [HL2641] detects HAP40 protein at cytoplasm by immunofluorescent analysis.
Sample: 293T cells were fixed in ice-cold MeOH for 5 min.
Green: HAP40 stained by HAP40 antibody [HL2641] (GTX639097) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HAP40 antibody [HL2641] (GTX639097) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HAP40 antibody [HL2641] (GTX639097) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.