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HARS antibody [N1C1]

Anti-HARS antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX114032
Anti-HARS antibody [N1C1] used in Western Blot (WB). GTX114032

Cat No. GTX114032

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

HepG2 , HCT116

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.89 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HARS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Histidyl-Trna Synthetase,Cmt2W,Hrs,Ush3B,Hars

Cellular Localization

Cytoplasm

Background

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. [provided by RefSeq]

Database

DATA IMAGES
Anti-HARS antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX114032

GTX114032 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using HARS(GTX114032) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HARS antibody [N1C1] used in Western Blot (WB). GTX114032

GTX114032 WB Image

HARS antibody [N1C1] detects HARS protein by Western blot analysis.
A. 30 µg HepG2 whole cell lysate/extract
B. 30 µg HCT116 whole cell lysate/extract
7.5 % SDS-PAGE
HARS antibody [N1C1] (GTX114032) dilution: 1:500

REFERENCE
There are currently no references for HARS antibody [N1C1] (GTX114032). Be the first to share your publications with this product.
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169