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HARS2 antibody

Anti-HARS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX32644
Anti-HARS2 antibody used in Western Blot (WB). GTX32644
Anti-HARS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX32644

Cat No. GTX32644

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($329)
See all HARS2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 217-506 of human HARS2 (NP_036340.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

histidyl-tRNA synthetase 2, mitochondrial , HARSL , HARSR , HO3 , HisRS , PRLTS2

Cellular Localization

Mitochondrion matrix

Background

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is an enzyme belonging to the class II family of aminoacyl-tRNA synthetases. Functioning in the synthesis of histidyl-transfer RNA, the enzyme plays an accessory role in the regulation of protein biosynthesis. The gene is located in a head-to-head orientation with HARS on chromosome five, where the homologous genes likely share a bidirectional promoter. Mutations in this gene are associated with the pathogenesis of Perrault syndrome, which involves ovarian dysgenesis and sensorineural hearing loss. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-HARS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX32644

GTX32644 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX32644 HARS2 antibody.
Dilution : 1:100

Anti-HARS2 antibody used in Western Blot (WB). GTX32644

GTX32644 WB Image

WB analysis of various samples using GTX32644 HARS2 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-HARS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX32644

GTX32644 IHC-P Image

IHC-P analysis of rat brain tissue using GTX32644 HARS2 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 329