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HAX1 antibody

Anti-HAX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101992
Anti-HAX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101992
Anti-HAX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101992
Anti-HAX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101992
Anti-HAX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101992
Anti-HAX1 antibody used in Western Blot (WB). GTX101992

Cat. No. GTX101992

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all HAX1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

Molt-4 , Raji

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HAX1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037140

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HCLS1 associated protein X-1 , HCLSBP1 , HS1BP1 , SCN3

Cellular Localization

Mitochondrion , Endoplasmic reticulum , Nucleus membrane

Background

The protein encoded by this gene is known to associate with hematopoietic cell-specific Lyn substrate 1, a substrate of Src family tyrosine kinases. It also interacts with the product of the polycystic kidney disease 2 gene, mutations in which are associated with autosomal-dominant polycystic kidney disease, and with the F-actin-binding protein, cortactin. It was earlier thought that this gene product is mainly localized in the mitochondria, however, recent studies indicate it to be localized in the cell body. Mutations in this gene result in autosomal recessive severe congenital neutropenia, also known as Kostmann disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HAX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101992

GTX101992 IHC-P Image

HAX1 antibody detects HAX1 protein at cytoplasm in rat ovary by immunohistochemical analysis.
Sample: Paraffin-embedded rat ovary.
HAX1 antibody (GTX101992) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HAX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101992

GTX101992 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 Xenograft , using HAX1(GTX101992) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HAX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101992

GTX101992 ICC/IF Image

HAX1 antibody detects HAX1 protein at cell membrane by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: HAX1 stained by HAX1 antibody (GTX101992) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-HAX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101992

GTX101992 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using HAX1(GTX101992) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-HAX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101992

GTX101992 ICC/IF Image

HAX1 antibody detects HAX1 protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: HAX1 stained by HAX1 antibody (GTX101992) diluted at 1:500.

Anti-HAX1 antibody used in Western Blot (WB). GTX101992

GTX101992 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HAX1 antibody (GTX101992) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Berger CN et al. PLoS Pathog 2018; 14 (10) : e1007406 The Citrobacter rodentium type III secretion system effector EspO affects mucosal damage repair and antimicrobial responses.
Application : WB
Reactivity : Mouse
Hai J et al. Sci Rep 2017; 7 : 39692 TRIM14 is a Putative Tumor Suppressor and Regulator of Innate Immune Response in Non-Small Cell Lung Cancer.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169