GeneTex
United States (US)

HAX1 antibody

GTX101992_40128_IFA_19011506_138.jpg
GTX101992_IHC_19011506_808.jpg
GTX101992_40128_20150529_IHC_R_19011506_928.jpg
GTX101992_40128_WB_19011506_165.jpg
GTX101992_WB_19011506_436.jpg

Cat No. GTX101992

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

Molt-4 , Raji , 293T , A431 ,HeLa , HepG2

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HAX1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Hcls1 Associated Protein X-1,Hclsbp1,Hs1Bp1,Scn3,Hax1

Cellular Localization

Mitochondrion , Endoplasmic reticulum , Nucleus membrane

Background

The protein encoded by this gene is known to associate with hematopoietic cell-specific Lyn substrate 1, a substrate of Src family tyrosine kinases. It also interacts with the product of the polycystic kidney disease 2 gene, mutations in which are associated with autosomal-dominant polycystic kidney disease, and with the F-actin-binding protein, cortactin. It was earlier thought that this gene product is mainly localized in the mitochondria, however, recent studies indicate it to be localized in the cell body. Mutations in this gene result in autosomal recessive severe congenital neutropenia, also known as Kostmann disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101992_40128_IFA_19011506_138.jpg

GTX101992 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using HAX1(GTX101992) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX101992_IHC_19011506_808.jpg

GTX101992 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 Xenograft , using HAX1(GTX101992) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101992_40128_20150529_IHC_R_19011506_928.jpg

GTX101992 IHC-P Image

HAX1 antibody detects HAX1 protein at cytoplasm in rat ovary by immunohistochemical analysis.
Sample: Paraffin-embedded rat ovary.
HAX1 antibody (GTX101992) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101992_40128_WB_19011506_165.jpg

GTX101992 WB Image

HAX1 antibody detects HAX1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with HAX1 antibody (GTX101992) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101992_WB_19011506_436.jpg

GTX101992 WB Image

Sample (30 µg of whole cell lysate)
A: Molt-4 (GTX27912)
B: Raji
12% SDS PAGE
GTX101992 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169