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HAX1 antibody

Anti-HAX1 antibody used in Western Blot (WB). GTX54149
Anti-HAX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54149
Anti-HAX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54149

Cat. No. GTX54149

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all HAX1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:10 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-279 of human HAX1 (NP_006109.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HCLS1 associated protein X-1 , HCLSBP1 , HS1BP1 , SCN3

Cellular Localization

Mitochondrion,Endoplasmic reticulum,Cytoplasmic vesicle,Cell membrane,Nucleus

Background

The protein encoded by this gene is known to associate with hematopoietic cell-specific Lyn substrate 1, a substrate of Src family tyrosine kinases. It also interacts with the product of the polycystic kidney disease 2 gene, mutations in which are associated with autosomal-dominant polycystic kidney disease, and with the F-actin-binding protein, cortactin. It was earlier thought that this gene product is mainly localized in the mitochondria, however, recent studies indicate it to be localized in the cell body. Mutations in this gene result in autosomal recessive severe congenital neutropenia, also known as Kostmann disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-HAX1 antibody used in Western Blot (WB). GTX54149

GTX54149 WB Image

WB analysis of various sample lysates using GTX54149 HAX1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-HAX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54149

GTX54149 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX54149 HAX1 antibody.
Dilution : 1:100

Anti-HAX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54149

GTX54149 ICC/IF Image

ICC/IF analysis of A549 cells using GTX54149 HAX1 antibody.
Blue : DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399