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HB EGF antibody [4G10]

Overexpression
Anti-HB EGF antibody [4G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00702
Anti-HB EGF antibody [4G10] used in Immunoprecipitation (IP). GTX00702
Anti-HB EGF antibody [4G10] used in Western Blot (WB). GTX00702

Cat. No. GTX00702

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4G10

Isotype

 IgG1

Application

 WB, ICC/IF, IP, IHC, Neutralizing/Inhibition

Reactivity

 Human
Package
100 μg ($399)
See all HB EGF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-1 μg /ml
ICC/IF 5-10 μg/ml
IP 2 μg/ml
IHC Assay dependent
Neutralizing/Inhibition Assay dependent

Note :


It shows the Inhibition of HB-EGF ectoderm shedding and inhibition of DAP_007htheria Toxin binding to HB-EGFR.
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Product Note

We do not recommend use of this product for Mouse samples.

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human HB-EGF ectodomain expressed in SF21 cell

Purification

Purified IgG
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heparin binding EGF like growth factor , DTR , DTS , DTSF , HEGFL

Database

Research Area

DATA IMAGES

Anti-HB EGF antibody [4G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00702

GTX00702 ICC/IF Image

ICC/IF analysis of various samples using GTX00702 HB EGF antibody [4G10].
Vero-H : Vero cells overexpressing human HB-EGF protein
Vero-mH : Vero cells overexpressing mouse HB-EGF protein
Fixation : 4% PFA

Anti-HB EGF antibody [4G10] used in Immunoprecipitation (IP). GTX00702

GTX00702 IP Image

IP analysis of various samples using GTX00702 HB EGF antibody [4G10].
Lane 1 : Vero whole cell lysate
Lane 2 : Vero cells overexpressing human HB-EGF protein
Lane 3 : Vero cells overexpressing mouse HB-EGF protein

Anti-HB EGF antibody [4G10] used in Western Blot (WB). GTX00702

GTX00702 WB Image

Overexpression

WB analysis of various samples using GTX00702 HB EGF antibody [4G10].
HB-EGF is glycosylated at multiple sites and processed, which reflects multiple-forms in WB.
Lane 1 : Vero whole cell lysate
Lane 2 : Vero cells overexpressing human HB-EGF protein
Lane 3 : Vero cells overexpressing mouse HB-EGF protein

REFERENCE

Clone Reference

Hamaoka M et al. J Biochem. 2010; 148 (1):55-69 Anti-human HB-EGF monoclonal antibodies inhibiting ectodomain shedding of HB-EGF and diphtheria toxin binding.
Application : WB, ICC/IF, IP
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399