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HB EGF antibody [4G10]

Anti-HB EGF antibody [4G10] used in Immunoprecipitation (IP). GTX00702
Anti-HB EGF antibody [4G10] used in Western Blot (WB). GTX00702
Anti-HB EGF antibody [4G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00702

Cat No. GTX00702

Host

Mouse

Clonality

Monoclonal

Clone Name

4G10

Isotype

IgG1

Application

WB, ICC/IF, IP, IHC, Inhibition assay

Reactivity

Human
Package
100 μg ($359)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.2-1 μg /ml
ICC/IF 5-10 μg/ml
IP 2 μg/ml
IHC Assay dependent
Inhibition assay Assay dependent

Note :

Inhibition assay
It shows the Inhibition of HB-EGF ectoderm shedding and inhibition of DAP_007htheria Toxin binding to HB-EGFR.

Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Specificity/Sensitivity

This product does not react with Mouse.

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human HB-EGF ectodomain expressed in SF21 cell

Purification

Purified IgG
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

heparin binding EGF like growth factor , DTR , DTS , DTSF , HEGFL

Database

Research Area

DATA IMAGES

Anti-HB EGF antibody [4G10] used in Immunoprecipitation (IP). GTX00702

GTX00702 IP Image

IP analysis of various samples using GTX00702 HB EGF antibody [4G10].
Lane 1 : Vero whole cell lysate
Lane 2 : Vero cells overexpressing human HB-EGF protein
Lane 3 : Vero cells overexpressing mouse HB-EGF protein

Anti-HB EGF antibody [4G10] used in Western Blot (WB). GTX00702

GTX00702 WB Image

WB analysis of various samples using GTX00702 HB EGF antibody [4G10].
HB-EGF is glycosylated at multiple sites and processed, which reflects multiple-forms in WB.
Lane 1 : Vero whole cell lysate
Lane 2 : Vero cells overexpressing human HB-EGF protein
Lane 3 : Vero cells overexpressing mouse HB-EGF protein

Anti-HB EGF antibody [4G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00702

GTX00702 ICC/IF Image

ICC/IF analysis of various samples using GTX00702 HB EGF antibody [4G10].
Vero-H : Vero cells overexpressing human HB-EGF protein
Vero-mH : Vero cells overexpressing mouse HB-EGF protein
Fixation : 4% PFA

REFERENCE

REVIEW

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Package List Price ($)
$ 359