For ELISA: Use at a concentration of 0.5 - 1 μg/ml. For ELISA, the detection limit for human recombinant HB-EGF is ~0.03 ng/well. For Neut: Use at an assay dependent dilution. For WB: Use at a concentration of 1 - 2 μg/ml. For WB, the detection limit for human recombinant HB-EGF is ~0.5 ng/lane under non-reducing and reducing conditions. Predicted molecular weight: 23 kDa. Optimal dilutions/concentrations should be determined by the researcher.
Vascular smooth muscle cells.
This antibody will neutralize the biological activity of recombinant human HB EGF. It will not neutralize the biological activity of EGF, TGFalpha, or amphiregulin.
Lyophilized from 5% Trehalose, PBS, in 100μg BSAReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 0.25 mg/ml.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.25 mg/ml (Please refer to the vial label for the specific concentration.)
Purified recombinant human HB-EGF
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Heparin Binding Egf Like Growth Factor,Dtr,Dts,Dtsf,Hegfl,Hbegf
Type I membrane protein. Mature HB-EGF is released into the extracellular space and probably binds to a receptor.
Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a 22kDa O-glycosylated protein that is a potent mitogen and chemoattractant for vascular smooth muscle cells, fibroblasts and epithelial cells but not endothelial cells. The natural protein has an apparent molecular mass of 19-23 kDa and exists in multiple forms as a result of heterogeneous O-glycosylation and/or Nterminal truncation. HB-EGF is synthesized as a membrane-anchored precursor(proHB-EGF) that is proteolytically cleaved to release the soluble mature growth factor. The two forms are active as juxtacrine and paracrine/autocrine growth factors respectively.HB-EGF activates two EGF receptor subtypes, HER1/ErbB1 and HER4 and binds to heparan sulfate proteoglycan.