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HCE antibody [N1N3]

Anti-HCE antibody [N1N3] used in Western Blot (WB). GTX105694

Cat. No. GTX105694

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all HCE products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

HeLa , HepG2 , Molt-4

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HCE. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951733

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RNA guanylyltransferase and 5'-phosphatase , CAP1A , HCE , HCE1 , hCAP

Cellular Localization

Nucleus

Background

Bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus.

Database

Research Area

DATA IMAGES

Anti-HCE antibody [N1N3] used in Western Blot (WB). GTX105694

GTX105694 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
B: Hep G2 (GTX27900)
C: MOLT4 (GTX27912)
7.5% SDS PAGE
GTX105694 diluted at 1:1000

REFERENCE

Application Reference

Diamant G et al. Cell Rep 2012; 2 (4) : 722-31 DSIF restricts NF-κB signaling by coordinating elongation with mRNA processing of negative feedback genes.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169