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HCK antibody [3D12E10]

Anti-HCK antibody [3D12E10] used in IHC (Paraffin sections) (IHC-P). GTX83203
Anti-HCK antibody [3D12E10] used in Western Blot (WB). GTX83203

Cat. No. GTX83203

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3D12E10

Isotype

 IgG1

Application

 WB, IHC-P, ELISA

Reactivity

 Human
Package
100 μl ($409)
See all HCK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of HCK expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HCK proto-oncogene, Src family tyrosine kinase , JTK9 , p59Hck , p61Hck

Cellular Localization

Cytoplasm, cytosol,Cytoplasm, cytoskeleton,Golgi apparatus,Cytoplasmic vesicle,Lysosome,Nucleus

Background

The protein encoded by this gene is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Multiple isoforms with different subcellular distributions are produced due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) codon. [provided by RefSeq, Feb 2010]

Database

Research Area

DATA IMAGES

Anti-HCK antibody [3D12E10] used in IHC (Paraffin sections) (IHC-P). GTX83203

GTX83203 IHC-P Image

IHC-P analysis of human colon cancer (left) and ancreas cancer (right) using GTX83203 HCK antibody [3D12E10].

Anti-HCK antibody [3D12E10] used in Western Blot (WB). GTX83203

GTX83203 WB Image

WB analysis of truncated HCK recombinant protein (1) and full-length HCK-GFP transfected CHO-K1 cell lysate (2) using GTX83203 HCK antibody [3D12E10].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 409