APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human HCK. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
HCK proto-oncogene, Src family tyrosine kinase , JTK9 , p59Hck , p61Hck
Cellular Localization
Cytoplasm, cytosol,Cytoplasm, cytoskeleton,Golgi apparatus,Cytoplasmic vesicle,Lysosome,Nucleus
Background
The protein encoded by this gene is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Multiple isoforms with different subcellular distributions are produced due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) codon. [provided by RefSeq, Feb 2010]
Database
Research Area
DATA IMAGES
![Anti-HCK antibody [HL1673] used in Western Blot (WB). GTX637272](/templates/default/js/lightbox/images/loading.gif "Anti-HCK antibody [HL1673] used in Western Blot (WB). GTX637272")
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GTX637272 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HCK antibody [HL1673] (GTX637272) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637272 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with HCK antibody [HL1673] (GTX637272) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637272 WB Image
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HCK antibody [HL1673] (GTX637272) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637272 IHC-P Image
HCK antibody [HL1673] detects HCK protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human hepatocellular carcinoma.HCK stained by HCK antibody [HL1673] (GTX637272) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637272 IHC-P Image
HCK antibody [HL1673] detects HCK protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.HCK stained by HCK antibody [HL1673] (GTX637272) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
There are currently no references for HCK antibody [HL1673] (GTX637272). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for HCK antibody [HL1673] (GTX637272). Be the first to share your experience with this product.
![Anti-HCK antibody [HL1673] used in Western Blot (WB). GTX637272](/upload/website/prouct_img/small/GTX637272/GTX637272_44809_20220923_WB_22100302_508.webp "Anti-HCK antibody [HL1673] used in Western Blot (WB). GTX637272")
![Anti-HCK antibody [HL1673] used in Western Blot (WB). GTX637272](/upload/website/prouct_img/small/GTX637272/GTX637272_44809_20220930_WB_22101319_817.webp "Anti-HCK antibody [HL1673] used in Western Blot (WB). GTX637272")
![Anti-HCK antibody [HL1673] used in Western Blot (WB). GTX637272](/upload/website/prouct_img/small/GTX637272/GTX637272_T-44760_20220819_WB_M_tissue_22082402_811.webp "Anti-HCK antibody [HL1673] used in Western Blot (WB). GTX637272")
![Anti-HCK antibody [HL1673] used in IHC (Paraffin sections) (IHC-P). GTX637272](/upload/website/prouct_img/small/GTX637272/GTX637272_44809_20221223_IHC-P_22122722_724.webp "Anti-HCK antibody [HL1673] used in IHC (Paraffin sections) (IHC-P). GTX637272")
![Anti-HCK antibody [HL1673] used in IHC (Paraffin sections) (IHC-P). GTX637272](/upload/website/prouct_img/small/GTX637272/GTX637272_44809_20221223_IHC-P_M_22122722_258.webp "Anti-HCK antibody [HL1673] used in IHC (Paraffin sections) (IHC-P). GTX637272")
![Anti-HCK antibody [HL1673] used in Western Blot (WB). GTX637272](/upload/website/prouct_img/normal/GTX637272/GTX637272_44809_20220923_WB_22100302_508.webp "Anti-HCK antibody [HL1673] used in Western Blot (WB). GTX637272")
![Anti-HCK antibody [HL1673] used in Western Blot (WB). GTX637272](/upload/website/prouct_img/normal/GTX637272/GTX637272_44809_20220930_WB_22101319_817.webp "Anti-HCK antibody [HL1673] used in Western Blot (WB). GTX637272")
![Anti-HCK antibody [HL1673] used in Western Blot (WB). GTX637272](/upload/website/prouct_img/normal/GTX637272/GTX637272_T-44760_20220819_WB_M_tissue_22082402_811.webp "Anti-HCK antibody [HL1673] used in Western Blot (WB). GTX637272")
![Anti-HCK antibody [HL1673] used in IHC (Paraffin sections) (IHC-P). GTX637272](/upload/website/prouct_img/normal/GTX637272/GTX637272_44809_20221223_IHC-P_22122722_724.webp "Anti-HCK antibody [HL1673] used in IHC (Paraffin sections) (IHC-P). GTX637272")
![Anti-HCK antibody [HL1673] used in IHC (Paraffin sections) (IHC-P). GTX637272](/upload/website/prouct_img/normal/GTX637272/GTX637272_44809_20221223_IHC-P_M_22122722_258.webp "Anti-HCK antibody [HL1673] used in IHC (Paraffin sections) (IHC-P). GTX637272")