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HCK antibody

Anti-HCK antibody used in Western Blot (WB). GTX32645
Anti-HCK antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32645

Cat No. GTX32645

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($329)
See all HCK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:10 - 1:100
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human HCK (NP_001165600.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

HCK proto-oncogene, Src family tyrosine kinase , JTK9 , p59Hck , p61Hck

Cellular Localization

Cytoplasm, cytosol,Cytoplasm, cytoskeleton,Golgi apparatus,Cytoplasmic vesicle,Lysosome,Nucleus

Background

The protein encoded by this gene is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Multiple isoforms with different subcellular distributions are produced due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) codon. [provided by RefSeq, Feb 2010]

Database

Research Area

DATA IMAGES

Anti-HCK antibody used in Western Blot (WB). GTX32645

GTX32645 WB Image

WB analysis of HL-60 cell lysate using GTX32645 HCK antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-HCK antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32645

GTX32645 ICC/IF Image

ICC/IF analysis of A549 cell using GTX32645 HCK antibody.
Blue : DAPI

REFERENCE

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REVIEW

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Package List Price ($)
$ 329