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HCLS1 antibody [C3], C-term

Anti-HCLS1 antibody [C3], C-term used in Western Blot (WB). GTX100303
Anti-HCLS1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100303
Anti-HCLS1 antibody [C3], C-term used in Western Blot (WB). GTX100303

Cat. No. GTX100303

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all HCLS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

Jurkat , HL-60 , NIH-3T3 , JC

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human HCLS1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240928

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hematopoietic cell-specific Lyn substrate 1 , CTTNL , HS1 , lckBP1 , p75

Cellular Localization

Membrane; Peripheral membrane protein (Probable) , Cytoplasm (Probable) , Mitochondrion

Background

Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression.

Database

Research Area

DATA IMAGES

Anti-HCLS1 antibody [C3], C-term used in Western Blot (WB). GTX100303

GTX100303 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
B: HL-60
7.5% SDS PAGE
GTX100303 diluted at 1:10000

Anti-HCLS1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100303

GTX100303 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lymphocytes in gastric epithelium, using HCLS1(GTX100303) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HCLS1 antibody [C3], C-term used in Western Blot (WB). GTX100303

GTX100303 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
7.5% SDS PAGE
GTX100303 diluted at 1:5000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169