GeneTex
United States (US)

HCLS1 antibody [C3], C-term

GTX100303_39435_WB_M_19011506_372.jpg
GTX100303_39435_WB_19011506_544.jpg
GTX100303_39435_IHC_19011506_155.jpg

Cat No. GTX100303

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

Jurkat , HL-60 , NIH-3T3 , JC

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human HCLS1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CTTNL antibody, HS1 antibody, HCLS1 antibody, hematopoietic lineage cell-specific protein antibody, lckBP1 antibody, p75 antibody, hematopoietic cell-specific Lyn substrate 1 antibody

Cellular Localization

Membrane; Peripheral membrane protein (Probable) , Cytoplasm (Probable) , Mitochondrion

Background

Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression.

Database

DATA IMAGES
GTX100303_39435_WB_M_19011506_372.jpg

GTX100303 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
7.5% SDS PAGE
GTX100303 diluted at 1:5000

GTX100303_39435_WB_19011506_544.jpg

GTX100303 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
B: HL-60
7.5% SDS PAGE
GTX100303 diluted at 1:10000

GTX100303_39435_IHC_19011506_155.jpg

GTX100303 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lymphocytes in gastric epithelium, using HCLS1(GTX100303) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169