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HCLS1 antibody [N1N3]

Anti-HCLS1 antibody [N1N3] used in Immunoprecipitation (IP). GTX114467
Anti-HCLS1 antibody [N1N3] used in Western Blot (WB). GTX114467

Cat. No. GTX114467

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all HCLS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HCLS1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619113

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hematopoietic cell-specific Lyn substrate 1 , CTTNL , HS1 , lckBP1 , p75

Cellular Localization

Membrane; Peripheral membrane protein (Probable) , Cytoplasm (Probable) , Mitochondrion

Background

Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression.

Database

Research Area

DATA IMAGES

Anti-HCLS1 antibody [N1N3] used in Immunoprecipitation (IP). GTX114467

GTX114467 IP Image

HCLS1 antibody immunoprecipitates HCLS1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 μg whole cell lysate/extract of HCLS1 protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of HCLS1 by 2.5 μg of HCLS1 antibody (GTX114467) 10% SDS-PAGE The immunoprecipitated HCLS1 protein was detected by HCLS1 antibody (GTX114467) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-HCLS1 antibody [N1N3] used in Western Blot (WB). GTX114467

GTX114467 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
10% SDS PAGE
HCLS1 antibody
GTX114467 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169