APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
SK-N-AS , mouse brain , rat brain
Predict Reactivity
Rabbit, Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.45 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human HCN1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886463
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
hyperpolarization activated cyclic nucleotide gated potassium channel 1 , BCNG-1 , BCNG1 , EIEE24 , HAC-2
Cellular Localization
Membrane; Multi-pass membrane protein
Background
Hyperpolarization-activated cation channels of the HCN gene family, such as HCN1, contribute to spontaneous rhythmic activity in both heart and brain.[supplied by OMIM]
Database
Research Area
DATA IMAGES
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GTX131334 IHC-P Image
HCN1 antibody detects HCN1 protein at cell membrane in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
HCN1 antibody (GTX131334) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX131334 WB Image
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HCN1 antibody (GTX131334) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX131334 IHC-Fr Image
HCN1 antibody detects HCN1 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: HCN1 protein stained by HCN1 antibody (GTX131334) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX131334 IHC-P Image
HCN1 antibody detects HCN1 protein at cell membrane in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
HCN1 antibody (GTX131334) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX131334 ICC/IF Image
HCN1 antibody detects HCN1 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HCN1 stained by HCN1 antibody (GTX131334) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX131334 IHC-P Image
HCN1 antibody detects HCN1 protein in mouse retina by immunohistochemical analysis.
Sample: Paraffin-embedded mouse retina.
HCN1 antibody (GTX131334) diluted at 1:800.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX131334 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HCN1 antibody (GTX131334) diluted at 1:1000.
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GTX131334 IHC-Fr Image
HCN1 antibody detects HCN1 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse hippocampus.
Green: HCN1 protein stained by HCN1 antibody (GTX131334) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
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REFERENCE
There are currently no references for HCN1 antibody (GTX131334). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for HCN1 antibody (GTX131334). Be the first to share your experience with this product.
![Anti-SOX2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101507](/upload/website/prouct_img/small/GTX101507/GTX101507_42046_20150814_IHC-P_M_19011506_157.jpg "Anti-SOX2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101507")
