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HCN1 antibody

Anti-HCN1 antibody used in Western Blot (WB). GTX131334
Anti-HCN1 antibody used in Western Blot (WB). GTX131334
Anti-HCN1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX131334
Anti-HCN1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX131334
Anti-HCN1 antibody used in IHC (Paraffin sections) (IHC-P). GTX131334
Anti-HCN1 antibody used in IHC (Paraffin sections) (IHC-P). GTX131334
Anti-HCN1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131334
Anti-HCN1 antibody used in IHC (Paraffin sections) (IHC-P). GTX131334

Cat. No. GTX131334

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all HCN1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

99 kDa. ( Note )

Positive Control

SK-N-AS , mouse brain , rat brain

Predict Reactivity

Rabbit, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of human HCN1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886463

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hyperpolarization activated cyclic nucleotide gated potassium channel 1 , BCNG-1 , BCNG1 , EIEE24 , HAC-2

Cellular Localization

Membrane; Multi-pass membrane protein

Background

Hyperpolarization-activated cation channels of the HCN gene family, such as HCN1, contribute to spontaneous rhythmic activity in both heart and brain.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-HCN1 antibody used in Western Blot (WB). GTX131334

GTX131334 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HCN1 antibody (GTX131334) diluted at 1:1000.

Anti-HCN1 antibody used in Western Blot (WB). GTX131334

GTX131334 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HCN1 antibody (GTX131334) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HCN1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX131334

GTX131334 IHC-Fr Image

HCN1 antibody detects HCN1 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: HCN1 protein stained by HCN1 antibody (GTX131334) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-HCN1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX131334

GTX131334 IHC-Fr Image

HCN1 antibody detects HCN1 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse hippocampus.
Green: HCN1 protein stained by HCN1 antibody (GTX131334) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-HCN1 antibody used in IHC (Paraffin sections) (IHC-P). GTX131334

GTX131334 IHC-P Image

HCN1 antibody detects HCN1 protein at cell membrane in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
HCN1 antibody (GTX131334) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HCN1 antibody used in IHC (Paraffin sections) (IHC-P). GTX131334

GTX131334 IHC-P Image

HCN1 antibody detects HCN1 protein at cell membrane in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
HCN1 antibody (GTX131334) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HCN1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131334

GTX131334 ICC/IF Image

HCN1 antibody detects HCN1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HCN1 stained by HCN1 antibody (GTX131334) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-HCN1 antibody used in IHC (Paraffin sections) (IHC-P). GTX131334

GTX131334 IHC-P Image

HCN1 antibody detects HCN1 protein in mouse retina by immunohistochemical analysis.
Sample: Paraffin-embedded mouse retina.
HCN1 antibody (GTX131334) diluted at 1:800.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Monz?n-Sandoval J et al. Ann Neurol 2020; 87 (6) : 853-868 Human-Specific Transcriptome of Ventral and Dorsal Midbrain Dopamine Neurons.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169