Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.45 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human HCN1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886463
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Hyperpolarization-activated cation channels of the HCN gene family, such as HCN1, contribute to spontaneous rhythmic activity in both heart and brain.[supplied by OMIM]
HCN1 antibody detects HCN1 protein at cell membrane in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. HCN1 antibody (GTX131334) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX131334 WB Image
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HCN1 antibody (GTX131334) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX131334 IHC-Fr Image
HCN1 antibody detects HCN1 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: HCN1 protein stained by HCN1 antibody (GTX131334) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
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GTX131334 IHC-P Image
HCN1 antibody detects HCN1 protein at cell membrane in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. HCN1 antibody (GTX131334) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX131334 ICC/IF Image
HCN1 antibody detects HCN1 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HCN1 stained by HCN1 antibody (GTX131334) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX131334 IHC-P Image
HCN1 antibody detects HCN1 protein in mouse retina by immunohistochemical analysis. Sample: Paraffin-embedded mouse retina. HCN1 antibody (GTX131334) diluted at 1:800.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX131334 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HCN1 antibody (GTX131334) diluted at 1:1000.
Show More +
GTX131334 IHC-Fr Image
HCN1 antibody detects HCN1 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse hippocampus. Green: HCN1 protein stained by HCN1 antibody (GTX131334) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).