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HCN1 antibody

Anti-HCN1 antibody used in Western Blot (WB). GTX54823
Anti-HCN1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54823
Anti-HCN1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54823

Cat. No. GTX54823

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
50 μl ($639)
See all HCN1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.85 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Peptide (C)KPNSASNSRDDGNSVYPSK, corresponding to amino acid residues 6-24 (Intracellular, N-terminus) of rat HCN1 (Accession  : Q9JKB0).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hyperpolarization-activated cyclic nucleotide-gated potassium channel 1

Cellular Localization

Cell membrane

Background

may generate neuronal pacemaker activity [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-HCN1 antibody used in Western Blot (WB). GTX54823

GTX54823 WB Image

WB analysis of rat brain membrane lysates using GTX54823 HCN1 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

Anti-HCN1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54823

GTX54823 IHC-Fr Image

IHC-Fr analysis of mouse cerebellum tissue using GTX54823 HCN1 antibody.
Panel A : HCN1 (green) appears in the cerebellar pinceau.
Panel B : Calcium binding calbindin D28-K (red), a marker of Purkinje neurons, is stained in the same section.
Panel C : Merge of the images demonstrates the position of the HCN1-positive pinceau structures at the axon initial segment of Purkinje neurons.

Anti-HCN1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54823

GTX54823 ICC/IF Image

ICC/IF analysis of PFA-fixed rat DRG primary cells using GTX54823 HCN1 antibody.
Green : Primary antibody
Blue : DNA dye Hoechst 33342
Dilution : 1:300

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 639