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HCN2 antibody

Anti-HCN2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54824
Anti-HCN2 antibody used in Western Blot (WB). GTX54824
Anti-HCN2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54824
Anti-HCN2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54824
Anti-HCN2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54824

Cat No. GTX54824

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-Fr, IP

Reactivity

Human, Mouse, Rat
Package
50 μl ($549)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 1% BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide (C)EEAGPAGEPRGSQAS, corresponding to amino acid residues 147-161 (Intracellular, N-terminus) of human HCN2 (Accession  : Q9UL51).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

hyperpolarization activated cyclic nucleotide gated potassium and sodium channel 2 , BCNG-2 , BCNG2 , HAC-1

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a hyperpolarization-activated cation channel involved in the generation of native pacemaker activity in the heart and in the brain. The encoded protein is activated by cAMP and can produce a fast, large current. Defects in this gene were noted as a possible cause of some forms of epilepsy. [provided by RefSeq, Jan 2017]

Database

Research Area

DATA IMAGES

Anti-HCN2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54824

GTX54824 ICC/IF Image

ICC/IF analysis of PFA-fixed rat DRG primary cells using GTX54824 HCN2 antibody.
Green : Primary antibody
Blue : DNA dye Hoechst 33342
Dilution : 1:100

Anti-HCN2 antibody used in Western Blot (WB). GTX54824

GTX54824 WB Image

WB analysis of rat brain membrane lysate using GTX54824 HCN2 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

Anti-HCN2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54824

GTX54824 IHC-Fr Image

IHC-Fr analysis of mouse  hypothalamus tissue using GTX54824 HCN2 antibody. (V = Third ventricle)
Panel A : HCN2 (red) appears in cells of the paraventricular nucleus (PVN, arrows).
Panel B : Staining of paraventricular nerve cells with mouse anti-calcium binding protein (CBD28k, green).
Panel C : Merge of HCN2 and CBD28k demonstrates some co-localization.

Anti-HCN2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54824

GTX54824 IHC-Fr Image

IHC-Fr analysis of mouse thalamus tissue using GTX54824 HCN2 antibody.
Panel A : Staining of HCN4 (green) appears in the ventral posterior thalamic nucleus (VPL).
Panel B : In the same section as in A, staining of HCN2 (red) appears in the ventral posterior thalamic nucleus (VPL) and also in the reticular thalamic nucleus (RT). The area between these thalamic nuclei (star) is white matter and neither protein is expressed in that region.
Panel C : Merged images of A and B.

Anti-HCN2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54824

GTX54824 IHC-Fr Image

IHC-Fr analysis of rat cerebellum tissue using GTX54824 HCN2 antibody.
Panel A : HCN2 (red) appears in Purkinje cells (arrows).
Panel B : Staining of astrocytes with mouse anti-glial fibrillary acidic protein (GFAP, green) demonstrates the restriction of HCN2 to neuronal cell bodies.
Panel C : Merge of HCN2 and GFAP images demonstrates the respective localization of these proteins.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf