APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
| 1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS pH7.3, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from hmuan HDAC1
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
histone deacetylase 1 , GON-10 , HD1 , KDAC1 , RPD3 , RPD3L1
Cellular Localization
Nucleus
Background
Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-HDAC1 antibody [GT1163] used in Western Blot (WB). GTX08970](/templates/default/js/lightbox/images/loading.gif "Anti-HDAC1 antibody [GT1163] used in Western Blot (WB). GTX08970")
|
GTX08970 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody [GT1163] (GTX08970) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
|
|
|
GTX08970 WB Image
WB analysis of various samples using GTX08970 HDAC1 antibody [GT1163].
Dilution : 1:1000
Loading : 25μg
|
|
|
GTX08970 WB Image
WB analysis of Wild-type (WT) and HDAC1 knockout (KO) 293T cell extracts using GTX08970 HDAC1 antibody [GT1163].
Dilution : 1:1000
Loading : 25μg
|
|
|
GTX08970 IHC-P Image
IHC-P analysis of mouse brain tissue section using GTX08970 HDAC1 antibody [GT1163].
Dlution : 1:100
|
|
|
GTX08970 IHC-P Image
IHC-P analysis of rat testis tissue section using GTX08970 HDAC1 antibody [GT1163].
Dlution : 1:100
|
|
|
GTX08970 ICC/IF Image
ICC/IF analysis of C6 cells using GTX08970 HDAC1 antibody [GT1163].
Orange: Primary antibody
Blue: DAPI
Dlution : 1:100
|
|
|
GTX08970 ICC/IF Image
ICC/IF analysis of NIH3T3 cells using GTX08970 HDAC1 antibody [GT1163].
Orange: Primary antibody
Blue: DAPI
Dlution : 1:100
|
REFERENCE
There are currently no references for HDAC1 antibody [GT1163] (GTX08970). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for HDAC1 antibody [GT1163] (GTX08970). Be the first to share your experience with this product.
![Anti-HDAC1 antibody [GT1163] used in Western Blot (WB). GTX08970](/upload/website/prouct_img/small/GTX08970/GTX08970_40000000050_20200306_WB_watermark_TPM_20031101_578.jpg "Anti-HDAC1 antibody [GT1163] used in Western Blot (WB). GTX08970")
![Anti-HDAC1 antibody [GT1163] used in Western Blot (WB). GTX08970](/upload/website/prouct_img/small/GTX08970/GTX08970_20200327_WB_1_20040622_821.jpg "Anti-HDAC1 antibody [GT1163] used in Western Blot (WB). GTX08970")
![Anti-HDAC1 antibody [GT1163] used in Western Blot (WB). GTX08970](/upload/website/prouct_img/small/GTX08970/GTX08970_20200327_WB_2_20040622_850.jpg "Anti-HDAC1 antibody [GT1163] used in Western Blot (WB). GTX08970")
![Anti-HDAC1 antibody [GT1163] used in IHC (Paraffin sections) (IHC-P). GTX08970](/upload/website/prouct_img/small/GTX08970/GTX08970_20191101_AP_002_87_20040622_658.jpg "Anti-HDAC1 antibody [GT1163] used in IHC (Paraffin sections) (IHC-P). GTX08970")
![Anti-HDAC1 antibody [GT1163] used in IHC (Paraffin sections) (IHC-P). GTX08970](/upload/website/prouct_img/small/GTX08970/GTX08970_20191101_AP_002_86_20040622_345.jpg "Anti-HDAC1 antibody [GT1163] used in IHC (Paraffin sections) (IHC-P). GTX08970")
![Anti-HDAC1 antibody [GT1163] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX08970](/upload/website/prouct_img/small/GTX08970/GTX08970_20191101_AP_003_84_20040622_331.jpg "Anti-HDAC1 antibody [GT1163] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX08970")
![Anti-HDAC1 antibody [GT1163] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX08970](/upload/website/prouct_img/small/GTX08970/GTX08970_20191101_AP_003_85_20040622_759.jpg "Anti-HDAC1 antibody [GT1163] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX08970")
![Anti-HDAC1 antibody [GT1163] used in Western Blot (WB). GTX08970](/upload/website/prouct_img/normal/GTX08970/GTX08970_40000000050_20200306_WB_watermark_TPM_20031101_578.jpg "Anti-HDAC1 antibody [GT1163] used in Western Blot (WB). GTX08970")
![Anti-HDAC1 antibody [GT1163] used in Western Blot (WB). GTX08970](/upload/website/prouct_img/normal/GTX08970/GTX08970_20200327_WB_1_20040622_821.jpg "Anti-HDAC1 antibody [GT1163] used in Western Blot (WB). GTX08970")
![Anti-HDAC1 antibody [GT1163] used in Western Blot (WB). GTX08970](/upload/website/prouct_img/normal/GTX08970/GTX08970_20200327_WB_2_20040622_850.jpg "Anti-HDAC1 antibody [GT1163] used in Western Blot (WB). GTX08970")
![Anti-HDAC1 antibody [GT1163] used in IHC (Paraffin sections) (IHC-P). GTX08970](/upload/website/prouct_img/normal/GTX08970/GTX08970_20191101_AP_002_87_20040622_658.jpg "Anti-HDAC1 antibody [GT1163] used in IHC (Paraffin sections) (IHC-P). GTX08970")
![Anti-HDAC1 antibody [GT1163] used in IHC (Paraffin sections) (IHC-P). GTX08970](/upload/website/prouct_img/normal/GTX08970/GTX08970_20191101_AP_002_86_20040622_345.jpg "Anti-HDAC1 antibody [GT1163] used in IHC (Paraffin sections) (IHC-P). GTX08970")
![Anti-HDAC1 antibody [GT1163] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX08970](/upload/website/prouct_img/normal/GTX08970/GTX08970_20191101_AP_003_84_20040622_331.jpg "Anti-HDAC1 antibody [GT1163] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX08970")
![Anti-HDAC1 antibody [GT1163] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX08970](/upload/website/prouct_img/normal/GTX08970/GTX08970_20191101_AP_003_85_20040622_759.jpg "Anti-HDAC1 antibody [GT1163] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX08970")