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HDAC1 antibody [GT1163]

Recombinant antibody KO/KD Orthogonal
Anti-HDAC1 antibody [GT1163] used in Western Blot (WB). GTX08970
Anti-HDAC1 antibody [GT1163] used in Western Blot (WB). GTX08970
Anti-HDAC1 antibody [GT1163] used in Western Blot (WB). GTX08970
Anti-HDAC1 antibody [GT1163] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX08970
Anti-HDAC1 antibody [GT1163] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX08970
Anti-HDAC1 antibody [GT1163] used in IHC (Paraffin sections) (IHC-P). GTX08970
Anti-HDAC1 antibody [GT1163] used in IHC (Paraffin sections) (IHC-P). GTX08970

Cat. No. GTX08970

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1163

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all HDAC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from hmuan HDAC1

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

histone deacetylase 1 , GON-10 , HD1 , KDAC1 , RPD3 , RPD3L1

Cellular Localization

Nucleus

Background

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-HDAC1 antibody [GT1163] used in Western Blot (WB). GTX08970

GTX08970 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody [GT1163] (GTX08970) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-HDAC1 antibody [GT1163] used in Western Blot (WB). GTX08970

GTX08970 WB Image

WB analysis of various samples using GTX08970 HDAC1 antibody [GT1163].
Dilution : 1:1000
Loading : 25μg

Anti-HDAC1 antibody [GT1163] used in Western Blot (WB). GTX08970

GTX08970 WB Image

KO/KD

WB analysis of Wild-type (WT) and HDAC1 knockout (KO) 293T cell extracts using GTX08970 HDAC1 antibody [GT1163].
Dilution : 1:1000
Loading : 25μg

Anti-HDAC1 antibody [GT1163] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX08970

GTX08970 ICC/IF Image

ICC/IF analysis of C6 cells using GTX08970 HDAC1 antibody [GT1163].
Orange: Primary antibody
Blue: DAPI
Dlution : 1:100

Anti-HDAC1 antibody [GT1163] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX08970

GTX08970 ICC/IF Image

ICC/IF analysis of NIH3T3 cells using GTX08970 HDAC1 antibody [GT1163].
Orange: Primary antibody
Blue: DAPI
Dlution : 1:100

Anti-HDAC1 antibody [GT1163] used in IHC (Paraffin sections) (IHC-P). GTX08970

GTX08970 IHC-P Image

IHC-P analysis of rat testis tissue section using GTX08970 HDAC1 antibody [GT1163].
Dlution : 1:100

Anti-HDAC1 antibody [GT1163] used in IHC (Paraffin sections) (IHC-P). GTX08970

GTX08970 IHC-P Image

IHC-P analysis of mouse brain tissue section using GTX08970 HDAC1 antibody [GT1163].
Dlution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399