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HDAC1 antibody

Anti-HDAC1 antibody used in Western Blot (WB). GTX100513
Anti-HDAC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100513
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513
Anti-HDAC1 antibody used in ChIP assay (ChIP assay). GTX100513
Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513
Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513
Anti-HDAC1 antibody used in Immunoprecipitation (IP). GTX100513
Anti-HDAC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100513
Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513
Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513
Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513
Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513

Cat No. GTX100513

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , U87-MG , SK-N-SH , NIH3T3 , Rat-2 , *E8.5 embryo

Predict Reactivity

Bovine, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HDAC1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

histone deacetylase 1 , GON-10 , HD1 , KDAC1 , RPD3 , RPD3L1

Cellular Localization

Nucleus

Background

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HDAC1 antibody used in Western Blot (WB). GTX100513

GTX100513 WB Image

Wild-type (WT) and HDAC1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100513

GTX100513 ICC/IF Image

HDAC1 antibody detects HDAC1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HDAC1 protein stained by HDAC1 antibody (GTX100513) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.

Anti-HDAC1 antibody used in Western Blot (WB). GTX100513

GTX100513 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC1 antibody used in Western Blot (WB). GTX100513

GTX100513 WB Image

HDAC1 antibody detects HDAC1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC1 antibody used in ChIP assay (ChIP assay). GTX100513

GTX100513 ChIP assay Image

HDAC1 antibody immunoprecipitates HDAC1 protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of HDAC1 antibody (GTX100513) The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.

Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513

GTX100513 IHC-P Image

HDAC1 antibody detects HDAC1 protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
HDAC1 antibody (GTX100513) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513

GTX100513 IHC-P Image

HDAC1 antibody detects HDAC1 protein at nucleus on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
HDAC1 antibody (GTX100513) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HDAC1 antibody used in Immunoprecipitation (IP). GTX100513

GTX100513 IP Image

HDAC1antibody immunoprecipitates HDAC1 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of HDAC1 protein by 2.5 μg of HDAC1 antibody (GTX100513) 10% SDS-PAGE The immunoprecipitated HDAC1 protein was detected by HDAC1 antibody (GTX100513) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-HDAC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100513

GTX100513 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using HDAC1(GTX100513) antibody at 1:200 dilution.

Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513

GTX100513 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human testis, using HDAC1(GTX100513) antibody(10 μg/ml).
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513

GTX100513 IHC-P Image

HDAC1 antibody detects HDAC1 protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
HDAC1 antibody (GTX100513) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513

GTX100513 IHC-P Image

HDAC1 antibody detects HDAC1 protein at nucleus in human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
HDAC1 antibody (GTX100513) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HDAC1 antibody used in Western Blot (WB). GTX100513

GTX100513 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC1 antibody used in Western Blot (WB). GTX100513

GTX100513 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC1 antibody used in Western Blot (WB). GTX100513

GTX100513 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513

GTX100513 IHC-P Image

HDAC1 antibody detects HDAC1 protein at nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Green: HDAC1 antibody (GTX100513) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HDAC1 antibody used in Western Blot (WB). GTX100513

GTX100513 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

HDAC1 antibody Cat No. GTX100513

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 30-Apr-2013
Score :
Application Tested : WB
Sample Species : Ms
Sample : E8.5 mouse embryo tissue
Amount used : 40μg
Blocking : 4°C, 2Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf