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HDAC1 antibody

KO/KD Orthogonal Overexpression
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513
Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513
Anti-HDAC1 antibody used in IHC (Whole mount) (IHC-Wm). GTX100513
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513
Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513
Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513
Anti-HDAC1 antibody used in Immunoprecipitation (IP). GTX100513
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513
Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513
Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513
Anti-HDAC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100513
Anti-HDAC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100513
Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513
Anti-HDAC1 antibody used in ChIP assay (ChIP assay). GTX100513
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513

Cat. No. GTX100513

Reference 33
10

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, IP, ChIP assay

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all HDAC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IHC-Wm Assay dependent
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , U87-MG , SK-N-SH , Rat-2 , NIH3T3 , DDDDK-tagged HDAC1-transfected 293T

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HDAC1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240929

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

histone deacetylase 1 , GON-10 , HD1 , KDAC1 , RPD3 , RPD3L1

Cellular Localization

Nucleus

Background

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HDAC1 antibody used in Western Blot (WB). GTX100513

GTX100513 WB Image

KO/KD

Wild-type (WT) and HDAC1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC1 antibody used in Western Blot (WB). GTX100513

GTX100513 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC1 antibody used in Western Blot (WB). GTX100513

GTX100513 WB Image

HDAC1 antibody detects HDAC1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513

GTX100513 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using HDAC1 antibody (GTX100513) at 1:300 dilution.

Anti-HDAC1 antibody used in Western Blot (WB). GTX100513

GTX100513 WB Image

Sample (30 μg of whole cell lysate)
A: zebrafish eye
10% SDS PAGE
GTX100513 diluted at 1:1000

Anti-HDAC1 antibody used in IHC (Whole mount) (IHC-Wm). GTX100513

GTX100513 IHC-Wm Image

HDAC1 antibody detects Hdac1 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
HDAC1 antibody (GTX100513) dilution: 1:100.
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513

GTX100513 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513

GTX100513 IHC-P Image

HDAC1 antibody detects HDAC1 protein at nucleus on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
HDAC1 antibody (GTX100513) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513

GTX100513 IHC-P Image

HDAC1 antibody detects HDAC1 protein at nucleus in human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
HDAC1 antibody (GTX100513) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HDAC1 antibody used in Immunoprecipitation (IP). GTX100513

GTX100513 IP Image

HDAC1antibody immunoprecipitates HDAC1 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of HDAC1 protein by 2.5 μg of HDAC1 antibody (GTX100513) 10% SDS-PAGE The immunoprecipitated HDAC1 protein was detected by HDAC1 antibody (GTX100513) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-HDAC1 antibody used in Western Blot (WB). GTX100513

GTX100513 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC1 antibody used in Western Blot (WB). GTX100513

GTX100513 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513

GTX100513 IHC-P Image

HDAC1 antibody detects HDAC1 protein at nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Green: HDAC1 antibody (GTX100513) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513

GTX100513 IHC-P Image

HDAC1 antibody detects HDAC1 protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
HDAC1 antibody (GTX100513) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HDAC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100513

GTX100513 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using HDAC1(GTX100513) antibody at 1:200 dilution.

Anti-HDAC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100513

GTX100513 ICC/IF Image

HDAC1 antibody detects HDAC1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HDAC1 stained by HDAC1 antibody (GTX100513) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Scale bar= 10μm.

Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513

GTX100513 IHC-P Image

HDAC1 antibody detects HDAC1 protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
HDAC1 antibody (GTX100513) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HDAC1 antibody used in ChIP assay (ChIP assay). GTX100513

GTX100513 ChIP assay Image

HDAC1 antibody immunoprecipitates HDAC1 protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of HDAC1 antibody (GTX100513) The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.

Anti-HDAC1 antibody used in Western Blot (WB). GTX100513

GTX100513 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Xu Z et al. Cell Death Discov 2022; 8 (1) : 474 Acetylation of Checkpoint suppressor 1 enhances its stability and promotes the progression of triple-negative breast cancer.
Oliver Clau? et al. Pharmaceuticals (Basel) 2022; 15 (3) Development and Biological Evaluation of the First Highly Potent and Specific Benzamide-Based Radiotracer [18F]BA3 for Imaging of Histone Deacetylases 1 and 2 in Brain.
Application : ICC/IF
Reactivity : Human
Lin HY et al. Biomed Pharmacother 2022; 145 : 112431 Epigenetic therapy combination of UNC0638 and CI-994 suppresses breast cancer via epigenetic remodeling of BIRC5 and GADD45A.
Application : WB
Reactivity : Human
Hang Yuan et al. Int J Mol Sci 2021; 22 (16) PPARγ-Induced Global H3K27 Acetylation Maintains Osteo/Cementogenic Abilities of Periodontal Ligament Fibroblasts.
Application : WB
Reactivity : Human
Abdollahi S et al. Biomark Res 2021; 9 (1) : 74 Deciphering genes associated with diffuse large B-cell lymphoma with lymphomatous effusions: A mutational accumulation scoring approach.
Application : IHC-P
Reactivity : Human
Show More +
Submit a Reference

REVIEW

HDAC1 antibody Cat. No. GTX100513

Rating
( Average 4.9 based on 10 users reviews)
Application
Western Blot(WB)
( Average 4.9 based on 7 users reviews)
IHC (Frozen sections)(IHC-Fr)
( Average 5 based on 3 users reviews)
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2018.PMID: 30242288
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Front Pharmacol in 2016.PMID: 27065869
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2019.PMID: 30934807
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS Biol in 2015.PMID: 26406915
Anti-HDAC1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX100513
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal J Cell Mol Med in 2020.PMID: 32227584
Anti-HDAC1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX100513
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS Biol in 2015.PMID: 26406915
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2018.PMID: 30242288
Anti-HDAC1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX100513
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2017. PMID: 28139683
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2017. PMID: 28139683
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513
Date : Anonymous submitted on 30-Apr-2013
Score :
Application Tested : WB
Sample Species : Ms
Sample : E8.5 mouse embryo tissue
Amount used : 40μg
Blocking : 4°C, 2Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Show More +
Submit a Review
Application Reference
Xu Z et al. Cell Death Discov 2022; 8 (1):474 Acetylation of Checkpoint suppressor 1 enhances its stability and promotes the progression of triple-negative breast cancer.
Oliver Clau? et al. Pharmaceuticals (Basel) 2022; 15 (3) Development and Biological Evaluation of the First Highly Potent and Specific Benzamide-Based Radiotracer [18F]BA3 for Imaging of Histone Deacetylases 1 and 2 in Brain.
Application : ICC/IF
Reactivity : Human
Lin HY et al. Biomed Pharmacother 2022; 145:112431 Epigenetic therapy combination of UNC0638 and CI-994 suppresses breast cancer via epigenetic remodeling of BIRC5 and GADD45A.
Application : WB
Reactivity : Human
Hang Yuan et al. Int J Mol Sci 2021; 22 (16) PPARγ-Induced Global H3K27 Acetylation Maintains Osteo/Cementogenic Abilities of Periodontal Ligament Fibroblasts.
Application : WB
Reactivity : Human
Abdollahi S et al. Biomark Res 2021; 9 (1):74 Deciphering genes associated with diffuse large B-cell lymphoma with lymphomatous effusions: A mutational accumulation scoring approach.
Application : IHC-P
Reactivity : Human
Chen K et al. Cancer Commun (Lond) 2021; 41 (4):316-332 Junction plakoglobin regulates and destabilizes HIF2α to inhibit tumorigenesis of renal cell carcinoma.
Application : WB
Reactivity : Human
Lin YC et al. Int J Mol Sci 2020; 21 (12) Nifedipine Exacerbates Lipogenesis in the Kidney via KIM-1, CD36, and SREBP Upregulation: Implications from an Animal Model for Human Study.
Ochiai H et al. Sci Adv 2020; 6 (25):eaaz6699 Genome-wide kinetic properties of transcriptional bursting in mouse embryonic stem cells.
Hu TM et al. Brain Sci 2020; 10 (8) Differential Expression of Multiple Disease-Related Protein Groups Induced by Valproic Acid in Human SH-SY5Y Neuroblastoma Cells.
Application : WB
Reactivity : Human
Deng M et al. J Cell Mol Med 2020; 24 (9):5249-5259 Overexpression of peptidase inhibitor 16 attenuates angiotensin II-induced cardiac fibrosis via regulating HDAC1 of cardiac fibroblasts.
Application : IHC-Fr
Reactivity : Mouse, Rat
Chen CY et al. Front Oncol 2020; 10:216 Hydroxygenkwanin Inhibits Class I HDAC Expression and Synergistically Enhances the Antitumor Activity of Sorafenib in Liver Cancer Cells.
Application : WB
Reactivity : Human
Gao Z et al. RNA Biol 2019; 16 (7):940-949 A novel human lncRNA SANT1 cis-regulates the expression of SLC47A2 by altering SFPQ/E2F1/HDAC1 binding to the promoter region in renal cell carcinoma.
Wu TH et al. Int J Mol Sci 2019; 20 (23) Emetine Synergizes with Cisplatin to Enhance Anti-Cancer Efficacy against Lung Cancer Cells.
Application : WB
Reactivity : Human
Lin YC et al. Int J Mol Sci 2019; 20 (7) Nifedipine Modulates Renal Lipogenesis via the AMPK-SREBP Transcriptional Pathway.
2019; (Epub) Loss of ARID1A induces a stemness gene ALDH1A1 expression with histone acetylation in the malignant subtype of cholangiocarcinoma.
Application : IP
Yokoji-Takeuchi M et al. Arch Oral Biol 2019; 110:104602 A bacterial metabolite induces Nrf2-mediated anti-oxidative responses in gingival epithelial cells by activating the MAPK signaling pathway.
Application : WB
Reactivity : Human
Yuanyuan Ye et al. International Journal of Oncology 2019;:1019-1032 SIP1 serves a role in HBx?induced liver cancer growth and metastasis.
Application : WB
Reactivity : Human
Wei GJ et al. AAPS J 2019; 21 (5):86 A Tangeretin Derivative Inhibits the Growth of Human Prostate Cancer LNCaP Cells by Epigenetically Restoring p21 Gene Expression and Inhibiting Cancer Stem-like Cell Proliferation.
Application : WB
Reactivity : Human
Jiao D et al. Oncogene 2019; (Epub) UHRF1 promotes renal cell carcinoma progression through epigenetic regulation of TXNIP.
Application : ChIP assay
Reactivity : Human
Liu Y et al. J Biol Chem 2018; 293 (36):14012-14021 Kr?ppel-like Factor 10 (KLF10) Is Transactivated by the Transcription Factor C/EBPβ and Involved in Early 3T3-L1 Preadipocyte Differentiation.
Lee HS et al. Nat Commun 2018; 9 (1):3848 The chromatin remodeler RSF1 controls centromeric histone modifications to coordinate chromosome segregation.
Application : WB
Reactivity : Human
Huang D et al. Nat Immunol 2018; (Epub) NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death.
Application : ChIP assay
Reactivity : Human
Sulijaya B et al. J Periodontal Res 2018; (Epub) The anti-inflammatory effect of 10-oxo-trans-11-octadecenoic acid (KetoC) on RAW 264.7 cells stimulated with Porphyromonas gingivalis lipopolysaccharide.
Application : WB
Reactivity : Mouse
Brugger V et al. Nat Commun 2017; 8:14272 Delaying histone deacetylase response to injury accelerates conversion into repair Schwann cells and nerve regeneration.
Lee JY et al. Front Pharmacol 2016; 7:81 Inhibition of HDAC3- and HDAC6-Promoted Survivin Expression Plays an Important Role in SAHA-Induced Autophagy and Viability Reduction in Breast Cancer Cells.
Application : WB
Reactivity : Human
Tsai HD et al. Neuromolecular Med 2016; (Epub) Clinacanthus nutans Protects Cortical Neurons Against Hypoxia-Induced Toxicity by Downregulating HDAC1/6.
Application : WB
Reactivity : Mouse
Baek MH et al. Anticancer Res 2016; 36 (5):2527-34 Immunohistochemical Characterization of Histone Deacetylase as a Potential Prognostic Marker and Therapeutic Target in Endometrial Stromal Sarcoma.
Application : IHC-P
Brugger V et al. PLoS Biol 2015; 13 (9):e1002258 HDAC1/2-Dependent P0 Expression Maintains Paranodal and Nodal Integrity Independently of Myelin Stability through Interactions with Neurofascins.
Application : WB, ICC/IF
Reactivity : Mouse
Lokireddy S et al. Proc Natl Acad Sci U S A 2015; 112 (52):E7176-85 cAMP-induced phosphorylation of 26S proteasomes on Rpn6/PSMD11 enhances their activity and the degradation of misfolded proteins.
Lee KH et al. Sci Rep 2014; 4:6394 A gene expression signature-based approach reveals the mechanisms of action of the Chinese herbal medicine berberine.
Application : WB
Reactivity : Human
Kumar S et al. Development 2014; 141 (15):391724 Retinoic acid controls body axis extension by directly repressing Fgf8 transcription.
Application : ChIP assay
Reactivity : Mouse
Chang JF et al. Toxicol Sci 2014; (139):396-406 nuclear proteomics with xrcc3 knockdown to reveal the development of *-resistant uterine cancer.
Application : WB
Reactivity : Human
Yoon JH et al. Biochem Pharmacol 2012; 83 (6):747-57 Role of autophagy in chemoresistance: regulation of the ATM-mediated DNA-damage signaling pathway through activation of DNA-PKcs and PARP-1.
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