GeneTex
  • Country / Location Selection

United States (US)

HDAC1 antibody

Anti-HDAC1 antibody used in Western Blot (WB). GTX100513
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513
Anti-HDAC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100513
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513
Anti-HDAC1 antibody used in ChIP assay (ChIP assay). GTX100513
Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513
Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513
Anti-HDAC1 antibody used in Immunoprecipitation (IP). GTX100513
Anti-HDAC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100513
Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513
Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513
Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513
Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513

Cat No. GTX100513

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, IP, ChIP assay

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , U87-MG , SK-N-SH , NIH3T3 , Rat-2 , *E8.5 embryo

Predict Reactivity

Bovine, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HDAC1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240929

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

histone deacetylase 1 , GON-10 , HD1 , KDAC1 , RPD3 , RPD3L1

Cellular Localization

Nucleus

Background

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HDAC1 antibody used in Western Blot (WB). GTX100513

GTX100513 WB Image

Wild-type (WT) and HDAC1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC1 antibody used in Western Blot (WB). GTX100513

GTX100513 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100513

GTX100513 ICC/IF Image

HDAC1 antibody detects HDAC1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HDAC1 protein stained by HDAC1 antibody (GTX100513) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.

Anti-HDAC1 antibody used in Western Blot (WB). GTX100513

GTX100513 WB Image

HDAC1 antibody detects HDAC1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC1 antibody used in ChIP assay (ChIP assay). GTX100513

GTX100513 ChIP assay Image

HDAC1 antibody immunoprecipitates HDAC1 protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of HDAC1 antibody (GTX100513) The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.

Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513

GTX100513 IHC-P Image

HDAC1 antibody detects HDAC1 protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
HDAC1 antibody (GTX100513) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513

GTX100513 IHC-P Image

HDAC1 antibody detects HDAC1 protein at nucleus on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
HDAC1 antibody (GTX100513) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HDAC1 antibody used in Immunoprecipitation (IP). GTX100513

GTX100513 IP Image

HDAC1antibody immunoprecipitates HDAC1 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of HDAC1 protein by 2.5 μg of HDAC1 antibody (GTX100513) 10% SDS-PAGE The immunoprecipitated HDAC1 protein was detected by HDAC1 antibody (GTX100513) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-HDAC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100513

GTX100513 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using HDAC1(GTX100513) antibody at 1:200 dilution.

Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513

GTX100513 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human testis, using HDAC1(GTX100513) antibody(10 μg/ml).
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513

GTX100513 IHC-P Image

HDAC1 antibody detects HDAC1 protein at nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Green: HDAC1 antibody (GTX100513) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HDAC1 antibody used in Western Blot (WB). GTX100513

GTX100513 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513

GTX100513 IHC-P Image

HDAC1 antibody detects HDAC1 protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
HDAC1 antibody (GTX100513) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513

GTX100513 IHC-P Image

HDAC1 antibody detects HDAC1 protein at nucleus in human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
HDAC1 antibody (GTX100513) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HDAC1 antibody used in Western Blot (WB). GTX100513

GTX100513 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC1 antibody used in Western Blot (WB). GTX100513

GTX100513 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC1 antibody used in Western Blot (WB). GTX100513

GTX100513 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

HDAC1 antibody Cat No. GTX100513

Rating ( Average 4.7 based on 3 users reviews)
Application
Western Blot(WB) ( Average 4.5 based on 2 users reviews)
IHC (Frozen sections)(IHC-Fr) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2017.PMID: 28139683
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2017.PMID: 28139683
Date : Anonymous submitted on 30-Apr-2013
Score :
Application Tested : WB
Sample Species : Ms
Sample : E8.5 mouse embryo tissue
Amount used : 40μg
Blocking : 4°C, 2Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf