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HDAC1 antibody

KO/KD Orthogonal Overexpression
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513
Anti-HDAC1 antibody used in ChIP assay (ChIP assay). GTX100513
Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513
Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513
Anti-HDAC1 antibody used in Immunoprecipitation (IP). GTX100513
Anti-HDAC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100513
Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513
Anti-HDAC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100513
Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513
Anti-HDAC1 antibody used in IHC (Whole mount) (IHC-Wm). GTX100513
Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513
Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513

Cat. No. GTX100513

Reference 32
10

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, IP, ChIP assay

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all HDAC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IHC-Wm Assay dependent
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , U87-MG , SK-N-SH , Rat-2 , NIH3T3 , DDDDK-tagged HDAC1-transfected 293T

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HDAC1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240929

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

histone deacetylase 1 , GON-10 , HD1 , KDAC1 , RPD3 , RPD3L1

Cellular Localization

Nucleus

Background

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HDAC1 antibody used in Western Blot (WB). GTX100513

GTX100513 WB Image

KO/KD

Wild-type (WT) and HDAC1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC1 antibody used in Western Blot (WB). GTX100513

GTX100513 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC1 antibody used in Western Blot (WB). GTX100513

GTX100513 WB Image

HDAC1 antibody detects HDAC1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC1 antibody used in ChIP assay (ChIP assay). GTX100513

GTX100513 ChIP assay Image

HDAC1 antibody immunoprecipitates HDAC1 protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of HDAC1 antibody (GTX100513) The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.

Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513

GTX100513 IHC-P Image

HDAC1 antibody detects HDAC1 protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
HDAC1 antibody (GTX100513) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513

GTX100513 IHC-P Image

HDAC1 antibody detects HDAC1 protein at nucleus on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
HDAC1 antibody (GTX100513) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HDAC1 antibody used in Immunoprecipitation (IP). GTX100513

GTX100513 IP Image

HDAC1antibody immunoprecipitates HDAC1 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of HDAC1 protein by 2.5 μg of HDAC1 antibody (GTX100513) 10% SDS-PAGE The immunoprecipitated HDAC1 protein was detected by HDAC1 antibody (GTX100513) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-HDAC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100513

GTX100513 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using HDAC1(GTX100513) antibody at 1:200 dilution.

Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513

GTX100513 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using HDAC1 antibody (GTX100513) at 1:300 dilution.

Anti-HDAC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100513

GTX100513 ICC/IF Image

HDAC1 antibody detects HDAC1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HDAC1 stained by HDAC1 antibody (GTX100513) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Scale bar= 10μm.

Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513

GTX100513 IHC-P Image

HDAC1 antibody detects HDAC1 protein at nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Green: HDAC1 antibody (GTX100513) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HDAC1 antibody used in Western Blot (WB). GTX100513

GTX100513 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC1 antibody used in Western Blot (WB). GTX100513

GTX100513 WB Image

Sample (30 μg of whole cell lysate)
A: zebrafish eye
10% SDS PAGE
GTX100513 diluted at 1:1000

Anti-HDAC1 antibody used in IHC (Whole mount) (IHC-Wm). GTX100513

GTX100513 IHC-Wm Image

HDAC1 antibody detects Hdac1 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
HDAC1 antibody (GTX100513) dilution: 1:100.
Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513

GTX100513 IHC-P Image

HDAC1 antibody detects HDAC1 protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
HDAC1 antibody (GTX100513) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HDAC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100513

GTX100513 IHC-P Image

HDAC1 antibody detects HDAC1 protein at nucleus in human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
HDAC1 antibody (GTX100513) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HDAC1 antibody used in Western Blot (WB). GTX100513

GTX100513 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC1 antibody used in Western Blot (WB). GTX100513

GTX100513 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC1 antibody used in Western Blot (WB). GTX100513

GTX100513 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Oliver Clau? et al. Pharmaceuticals (Basel) 2022; 15 (3) Development and Biological Evaluation of the First Highly Potent and Specific Benzamide-Based Radiotracer [18F]BA3 for Imaging of Histone Deacetylases 1 and 2 in Brain.
Application : ICC/IF
Reactivity : Human
Lin HY et al. Biomed Pharmacother 2022; 145 : 112431 Epigenetic therapy combination of UNC0638 and CI-994 suppresses breast cancer via epigenetic remodeling of BIRC5 and GADD45A.
Application : WB
Reactivity : Human
Hang Yuan et al. Int J Mol Sci 2021; 22 (16) PPARγ-Induced Global H3K27 Acetylation Maintains Osteo/Cementogenic Abilities of Periodontal Ligament Fibroblasts.
Application : WB
Reactivity : Human
Abdollahi S et al. Biomark Res 2021; 9 (1) : 74 Deciphering genes associated with diffuse large B-cell lymphoma with lymphomatous effusions: A mutational accumulation scoring approach.
Application : IHC-P
Reactivity : Human
Chen K et al. Cancer Commun (Lond) 2021; 41 (4) : 316-332 Junction plakoglobin regulates and destabilizes HIF2α to inhibit tumorigenesis of renal cell carcinoma.
Application : WB
Reactivity : Human
Show More +
Submit a Reference

REVIEW

HDAC1 antibody Cat. No. GTX100513

Rating
( Average 4.9 based on 10 users reviews)
Application
Western Blot(WB)
( Average 4.9 based on 7 users reviews)
IHC (Frozen sections)(IHC-Fr)
( Average 5 based on 3 users reviews)
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2018.PMID: 30242288
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Front Pharmacol in 2016.PMID: 27065869
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2019.PMID: 30934807
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS Biol in 2015.PMID: 26406915
Anti-HDAC1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX100513
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal J Cell Mol Med in 2020.PMID: 32227584
Anti-HDAC1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX100513
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS Biol in 2015.PMID: 26406915
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2018.PMID: 30242288
Anti-HDAC1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX100513
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2017. PMID: 28139683
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2017. PMID: 28139683
Anti-HDAC1 antibody used in Western Blot (WB). GTX100513
Date : Anonymous submitted on 30-Apr-2013
Score :
Application Tested : WB
Sample Species : Ms
Sample : E8.5 mouse embryo tissue
Amount used : 40μg
Blocking : 4°C, 2Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Show More +
Submit a Review
Application Reference
Oliver Clau? et al. Pharmaceuticals (Basel) 2022; 15 (3) Development and Biological Evaluation of the First Highly Potent and Specific Benzamide-Based Radiotracer [18F]BA3 for Imaging of Histone Deacetylases 1 and 2 in Brain.
Application : ICC/IF
Reactivity : Human
Lin HY et al. Biomed Pharmacother 2022; 145:112431 Epigenetic therapy combination of UNC0638 and CI-994 suppresses breast cancer via epigenetic remodeling of BIRC5 and GADD45A.
Application : WB
Reactivity : Human
Hang Yuan et al. Int J Mol Sci 2021; 22 (16) PPARγ-Induced Global H3K27 Acetylation Maintains Osteo/Cementogenic Abilities of Periodontal Ligament Fibroblasts.
Application : WB
Reactivity : Human
Abdollahi S et al. Biomark Res 2021; 9 (1):74 Deciphering genes associated with diffuse large B-cell lymphoma with lymphomatous effusions: A mutational accumulation scoring approach.
Application : IHC-P
Reactivity : Human
Chen K et al. Cancer Commun (Lond) 2021; 41 (4):316-332 Junction plakoglobin regulates and destabilizes HIF2α to inhibit tumorigenesis of renal cell carcinoma.
Application : WB
Reactivity : Human
Lin YC et al. Int J Mol Sci 2020; 21 (12) Nifedipine Exacerbates Lipogenesis in the Kidney via KIM-1, CD36, and SREBP Upregulation: Implications from an Animal Model for Human Study.
Ochiai H et al. Sci Adv 2020; 6 (25):eaaz6699 Genome-wide kinetic properties of transcriptional bursting in mouse embryonic stem cells.
Hu TM et al. Brain Sci 2020; 10 (8) Differential Expression of Multiple Disease-Related Protein Groups Induced by Valproic Acid in Human SH-SY5Y Neuroblastoma Cells.
Application : WB
Reactivity : Human
Deng M et al. J Cell Mol Med 2020; 24 (9):5249-5259 Overexpression of peptidase inhibitor 16 attenuates angiotensin II-induced cardiac fibrosis via regulating HDAC1 of cardiac fibroblasts.
Application : IHC-Fr
Reactivity : Mouse, Rat
Chen CY et al. Front Oncol 2020; 10:216 Hydroxygenkwanin Inhibits Class I HDAC Expression and Synergistically Enhances the Antitumor Activity of Sorafenib in Liver Cancer Cells.
Application : WB
Reactivity : Human
Gao Z et al. RNA Biol 2019; 16 (7):940-949 A novel human lncRNA SANT1 cis-regulates the expression of SLC47A2 by altering SFPQ/E2F1/HDAC1 binding to the promoter region in renal cell carcinoma.
Wu TH et al. Int J Mol Sci 2019; 20 (23) Emetine Synergizes with Cisplatin to Enhance Anti-Cancer Efficacy against Lung Cancer Cells.
Application : WB
Reactivity : Human
Lin YC et al. Int J Mol Sci 2019; 20 (7) Nifedipine Modulates Renal Lipogenesis via the AMPK-SREBP Transcriptional Pathway.
2019; (Epub) Loss of ARID1A induces a stemness gene ALDH1A1 expression with histone acetylation in the malignant subtype of cholangiocarcinoma.
Application : IP
Yokoji-Takeuchi M et al. Arch Oral Biol 2019; 110:104602 A bacterial metabolite induces Nrf2-mediated anti-oxidative responses in gingival epithelial cells by activating the MAPK signaling pathway.
Application : WB
Reactivity : Human
Yuanyuan Ye et al. International Journal of Oncology 2019;:1019-1032 SIP1 serves a role in HBx?induced liver cancer growth and metastasis.
Application : WB
Reactivity : Human
Wei GJ et al. AAPS J 2019; 21 (5):86 A Tangeretin Derivative Inhibits the Growth of Human Prostate Cancer LNCaP Cells by Epigenetically Restoring p21 Gene Expression and Inhibiting Cancer Stem-like Cell Proliferation.
Application : WB
Reactivity : Human
Jiao D et al. Oncogene 2019; (Epub) UHRF1 promotes renal cell carcinoma progression through epigenetic regulation of TXNIP.
Application : ChIP assay
Reactivity : Human
Liu Y et al. J Biol Chem 2018; 293 (36):14012-14021 Kr?ppel-like Factor 10 (KLF10) Is Transactivated by the Transcription Factor C/EBPβ and Involved in Early 3T3-L1 Preadipocyte Differentiation.
Lee HS et al. Nat Commun 2018; 9 (1):3848 The chromatin remodeler RSF1 controls centromeric histone modifications to coordinate chromosome segregation.
Application : WB
Reactivity : Human
Huang D et al. Nat Immunol 2018; (Epub) NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death.
Application : ChIP assay
Reactivity : Human
Sulijaya B et al. J Periodontal Res 2018; (Epub) The anti-inflammatory effect of 10-oxo-trans-11-octadecenoic acid (KetoC) on RAW 264.7 cells stimulated with Porphyromonas gingivalis lipopolysaccharide.
Application : WB
Reactivity : Mouse
Brugger V et al. Nat Commun 2017; 8:14272 Delaying histone deacetylase response to injury accelerates conversion into repair Schwann cells and nerve regeneration.
Lee JY et al. Front Pharmacol 2016; 7:81 Inhibition of HDAC3- and HDAC6-Promoted Survivin Expression Plays an Important Role in SAHA-Induced Autophagy and Viability Reduction in Breast Cancer Cells.
Application : WB
Reactivity : Human
Tsai HD et al. Neuromolecular Med 2016; (Epub) Clinacanthus nutans Protects Cortical Neurons Against Hypoxia-Induced Toxicity by Downregulating HDAC1/6.
Application : WB
Reactivity : Mouse
Baek MH et al. Anticancer Res 2016; 36 (5):2527-34 Immunohistochemical Characterization of Histone Deacetylase as a Potential Prognostic Marker and Therapeutic Target in Endometrial Stromal Sarcoma.
Application : IHC-P
Brugger V et al. PLoS Biol 2015; 13 (9):e1002258 HDAC1/2-Dependent P0 Expression Maintains Paranodal and Nodal Integrity Independently of Myelin Stability through Interactions with Neurofascins.
Application : WB, ICC/IF
Reactivity : Mouse
Lokireddy S et al. Proc Natl Acad Sci U S A 2015; 112 (52):E7176-85 cAMP-induced phosphorylation of 26S proteasomes on Rpn6/PSMD11 enhances their activity and the degradation of misfolded proteins.
Lee KH et al. Sci Rep 2014; 4:6394 A gene expression signature-based approach reveals the mechanisms of action of the Chinese herbal medicine berberine.
Application : WB
Reactivity : Human
Kumar S et al. Development 2014; 141 (15):391724 Retinoic acid controls body axis extension by directly repressing Fgf8 transcription.
Application : ChIP assay
Reactivity : Mouse
Chang JF et al. Toxicol Sci 2014; (139):396-406 nuclear proteomics with xrcc3 knockdown to reveal the development of *-resistant uterine cancer.
Application : WB
Reactivity : Human
Yoon JH et al. Biochem Pharmacol 2012; 83 (6):747-57 Role of autophagy in chemoresistance: regulation of the ATM-mediated DNA-damage signaling pathway through activation of DNA-PKcs and PARP-1.
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