Western blot - Use at 1:250 to 1:1,000 dilution. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry.
Reacts with the recombinant human HDAC 10 protein.
PBS containing 0.02% sodium azide.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Affinity purified IgG
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Histone Deacetylase 10 , Hd10 , Hdac10
A novel class II histone deacetylase, HDAC10 is most similar to HDAC6. Both contain a unique, putative second catalytic domain not found in other HDACs. In HDAC10, however, this domain is not functional. Unlike HDAC6, which is normally a cytoplasmic deacetylase, HDAC10 resides in both the nucleus and cytoplasm. Data suggests that HDAC10 may play roles both in the nucleus, as a transcriptional modulator, and in the cytoplasm in an unidentified role. HDAC10 variants seem present in certain cell lines and tissues that are as of yet not characterized.
GTX70464 WB Image
Western blot analysis of HDAC10 in HeLa cell lysate.