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HDAC2 antibody [GT1219]

Recombinant antibody
Anti-HDAC2 antibody [GT1219] used in Western Blot (WB). GTX01527
Anti-HDAC2 antibody [GT1219] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01527
Anti-HDAC2 antibody [GT1219] used in Western Blot (WB). GTX01527
Anti-HDAC2 antibody [GT1219] used in IHC (Paraffin sections) (IHC-P). GTX01527
Anti-HDAC2 antibody [GT1219] used in IHC (Paraffin sections) (IHC-P). GTX01527
Anti-HDAC2 antibody [GT1219] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01527
Anti-HDAC2 antibody [GT1219] used in IHC (Paraffin sections) (IHC-P). GTX01527

Cat. No. GTX01527

Reference 1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1219

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all HDAC2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human HDAC2.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HD2,HDAC2,RPD3,YAF1,histone deacetylase 2

Cellular Localization

Nucleus,Cytoplasm

Background

This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]

Database

Research Area

DATA IMAGES

Anti-HDAC2 antibody [GT1219] used in Western Blot (WB). GTX01527

GTX01527 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC2 antibody [GT1219] (GTX01527) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 27877082

Anti-HDAC2 antibody [GT1219] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01527

GTX01527 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX01527 HDAC2 antibody [GT1219].
Blue : DAPI

Anti-HDAC2 antibody [GT1219] used in Western Blot (WB). GTX01527

GTX01527 WB Image

WB analysis of various samples using GTX01527 HDAC2 antibody [GT1219].
Dilution : 1:1000
Loading : 25 μg

Anti-HDAC2 antibody [GT1219] used in IHC (Paraffin sections) (IHC-P). GTX01527

GTX01527 IHC-P Image

IHC-P analysis of mouse brain tissue section using GTX01527 HDAC2 antibody [GT1219].
Dilution : 1:100

Anti-HDAC2 antibody [GT1219] used in IHC (Paraffin sections) (IHC-P). GTX01527

GTX01527 IHC-P Image

IHC-P analysis of rat spleen tissue section using GTX01527 HDAC2 antibody [GT1219].
Dilution : 1:100

Anti-HDAC2 antibody [GT1219] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01527

GTX01527 ICC/IF Image

ICC/IF analysis of C6 cells using GTX01527 HDAC2 antibody [GT1219].
Blue : DAPI

Anti-HDAC2 antibody [GT1219] used in IHC (Paraffin sections) (IHC-P). GTX01527

GTX01527 IHC-P Image

IHC-P analysis of human oophoroma tissue section using GTX01527 HDAC2 antibody [GT1219].
Dilution : 1:100

REFERENCE

Application Reference

Yen YT et al. Nat Commun 2021; 12 (1) : 7297 Protein phosphatase 2A inactivation induces microsatellite instability, neoantigen production and immune response.
Application : WB, IHC-P
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399