APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Bovine, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human HDAC2. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
histone deacetylase 2 , HD2 , KDAC2 , RPD3 , YAF1
Cellular Localization
Nucleus,Cytoplasm
Background
This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]
Database
Research Area
DATA IMAGES
![Anti-HDAC2 antibody [HL1545] used in IHC (Paraffin sections) (IHC-P). GTX637020](/templates/default/js/lightbox/images/loading.gif "Anti-HDAC2 antibody [HL1545] used in IHC (Paraffin sections) (IHC-P). GTX637020")
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GTX637020 IHC-P Image
HDAC2 antibody [HL1545] detects HDAC2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lymph node.
HDAC2 stained by HDAC2 antibody [HL1545] (GTX637020) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637020 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC2 antibody [HL1545] (GTX637020) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637020 ICC/IF Image
HDAC2 antibody [HL1545] detects HDAC2 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HDAC2 stained by HDAC2 antibody [HL1545] (GTX637020) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
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GTX637020 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC2 antibody [HL1545] (GTX637020) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
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GTX637020 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC2 antibody [HL1545] (GTX637020) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for HDAC2 antibody [HL1545] (GTX637020). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for HDAC2 antibody [HL1545] (GTX637020). Be the first to share your experience with this product.
![Anti-HDAC2 antibody [HL1545] used in IHC (Paraffin sections) (IHC-P). GTX637020](/upload/website/prouct_img/small/GTX637020/GTX637020_T-44697_20220624_IHC-P_M_22062919_168.webp "Anti-HDAC2 antibody [HL1545] used in IHC (Paraffin sections) (IHC-P). GTX637020")
![Anti-HDAC2 antibody [HL1545] used in Western Blot (WB). GTX637020](/upload/website/prouct_img/small/GTX637020/GTX637020_44746_20220722_WB_22072519_316.webp "Anti-HDAC2 antibody [HL1545] used in Western Blot (WB). GTX637020")
![Anti-HDAC2 antibody [HL1545] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637020](/upload/website/prouct_img/small/GTX637020/GTX637020_T-44697_20220916_ICC_IF_22102723_542.webp "Anti-HDAC2 antibody [HL1545] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637020")
![Anti-HDAC2 antibody [HL1545] used in Western Blot (WB). GTX637020](/upload/website/prouct_img/small/GTX637020/GTX637020_T-44697_20220617_WB_M_22062121_543.webp "Anti-HDAC2 antibody [HL1545] used in Western Blot (WB). GTX637020")
![Anti-HDAC2 antibody [HL1545] used in Western Blot (WB). GTX637020](/upload/website/prouct_img/small/GTX637020/GTX637020_T-44697_20220617_WB_R_22062121_485.webp "Anti-HDAC2 antibody [HL1545] used in Western Blot (WB). GTX637020")
![Anti-HDAC2 antibody [HL1545] used in IHC (Paraffin sections) (IHC-P). GTX637020](/upload/website/prouct_img/normal/GTX637020/GTX637020_T-44697_20220624_IHC-P_M_22062919_168.webp "Anti-HDAC2 antibody [HL1545] used in IHC (Paraffin sections) (IHC-P). GTX637020")
![Anti-HDAC2 antibody [HL1545] used in Western Blot (WB). GTX637020](/upload/website/prouct_img/normal/GTX637020/GTX637020_44746_20220722_WB_22072519_316.webp "Anti-HDAC2 antibody [HL1545] used in Western Blot (WB). GTX637020")
![Anti-HDAC2 antibody [HL1545] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637020](/upload/website/prouct_img/normal/GTX637020/GTX637020_T-44697_20220916_ICC_IF_22102723_542.webp "Anti-HDAC2 antibody [HL1545] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637020")
![Anti-HDAC2 antibody [HL1545] used in Western Blot (WB). GTX637020](/upload/website/prouct_img/normal/GTX637020/GTX637020_T-44697_20220617_WB_M_22062121_543.webp "Anti-HDAC2 antibody [HL1545] used in Western Blot (WB). GTX637020")
![Anti-HDAC2 antibody [HL1545] used in Western Blot (WB). GTX637020](/upload/website/prouct_img/normal/GTX637020/GTX637020_T-44697_20220617_WB_R_22062121_485.webp "Anti-HDAC2 antibody [HL1545] used in Western Blot (WB). GTX637020")