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HDAC2 antibody

Anti-HDAC2 antibody used in Western Blot (WB). GTX113086
Anti-HDAC2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113086
Anti-HDAC2 antibody used in IHC (Paraffin sections) (IHC-P). GTX113086
Anti-HDAC2 antibody used in IHC (Paraffin sections) (IHC-P). GTX113086

Cat. No. GTX113086

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all HDAC2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

HeLa

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human HDAC2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618193

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

histone deacetylase 2 , HD2 , KDAC2 , RPD3 , YAF1

Cellular Localization

Nucleus

Background

This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues on the N-terminal region of the core histones (H2A, H2B, H3 and H4). This protein also forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus it plays an important role in transcriptional regulation, cell cycle progression and developmental events. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HDAC2 antibody used in Western Blot (WB). GTX113086

GTX113086 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
HDAC2 antibody
GTX113086 diluted at 1:1000

Anti-HDAC2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113086

GTX113086 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using HDAC2(GTX113086) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

Anti-HDAC2 antibody used in IHC (Paraffin sections) (IHC-P). GTX113086

GTX113086 IHC-P Image

HDAC2 antibody detects HDAC2 protein at nucleus on mouse middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse middle brain.
HDAC2 antibody (GTX113086) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HDAC2 antibody used in IHC (Paraffin sections) (IHC-P). GTX113086

GTX113086 IHC-P Image

HDAC2 antibody detects HDAC2 protein at nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
HDAC2 antibody (GTX113086) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169