APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.5 - 1 μg/mL |
| 5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
HeLa Cell Lysate
Product Note
At least two isoforms of HDAC2 are known to exist; this antibody will detect both isoforms. HDAC2 antibody is predicted to not cross-react with other members of the HDAC family.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
HDAC2 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human HDAC2.The immunogen is located within the last 50 amino acids of HDAC2.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
histone deacetylase 2 , HD2 , KDAC2 , RPD3 , YAF1
Cellular Localization
Nucleus,Cytoplasm
Background
This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]
Database
Research Area
DATA IMAGES
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GTX31925 WB Image
WB analysis of HeLa cell lysate using GTX31925 HDAC2 antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml
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GTX31925 IHC-P Image
IHC-P analysis of rat testis tissue using GTX31925 HDAC2 antibody.
Working concentration : 5 μg/ml
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GTX31925 IHC-P Image
IHC-P analysis of rat testis tissue using GTX31925 HDAC2 antibody.
Working concentration : 20 μg/ml
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REFERENCE
There are currently no references for HDAC2 antibody (GTX31925). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for HDAC2 antibody (GTX31925). Be the first to share your experience with this product.
