GeneTex
United States (US)

HDAC2 antibody

GTX31925_3073_WB_20180221_18121410_628.jpg
GTX31925_1597_IHC-P_20180221_18121410_485.jpg
GTX31925_1596_IHC-P_20180221_18121410_988.jpg

Cat No. GTX31925

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 - 1 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

HeLa Cell Lysate

Specificity/Sensitivity

At least two isoforms of HDAC2 are known to exist; this antibody will detect both isoforms. HDAC2 antibody is predicted to not cross-react with other members of the HDAC family.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

HDAC2 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human HDAC2.The immunogen is located within the last 50 amino acids of HDAC2.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Histone Deacetylase 2,Hd2,Rpd3,Yaf1,Hdac2

Background

This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]

Database

Research Area

DATA IMAGES
GTX31925_3073_WB_20180221_18121410_628.jpg

GTX31925 WB Image

WB analysis of HeLa cell lysate using GTX31925 HDAC2 antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml

GTX31925_1597_IHC-P_20180221_18121410_485.jpg

GTX31925 IHC-P Image

IHC-P analysis of rat testis tissue using GTX31925 HDAC2 antibody.
Working concentration : 20 μg/ml

GTX31925_1596_IHC-P_20180221_18121410_988.jpg

GTX31925 IHC-P Image

IHC-P analysis of rat testis tissue using GTX31925 HDAC2 antibody.
Working concentration : 5 μg/ml

Package List Price ($)
$ 329